Abstract
Long non-coding RNAs (lncRNAs) have crucial roles in the pathogenesis of immune-related disorders. However, their role in the pathobiology of inflammatory demyelinating polyradiculoneuropathies remains unclear. In the current study, we measured peripheral expression of four lncRNAs, namely TUG1, FAS-AS1, NEAT1, and GAS5, in patients with acute/chronic inflammatory demyelinating polyradiculoneuropathies (AIDP/CIDP) compared with healthy subjects. Notably, all lncRNAs were over-expressed in patients compared with controls (P < 0.0001 for all lncRNAs). When assessing their expressions in AIDP and CIDP groups separately, TUG1 and NEAT1 were up-regulated in both patient groups compared with controls, yet FAS-AS1 and GAS5 were only up-regulated in CIDP cases. There were remarkable pairwise correlations between expression levels of these lncRNAs in all study groups. Based on the above-mentioned data, we suggest participation of these for lncRNAs in the pathogenesis of inflammatory demyelinating polyradiculoneuropathies. Moreover, FAS-AS1 and GAS5 lncRNAs have type-specific roles in this regard. Future functional studies are needed to elaborate the molecular mechanisms of the contribution of these transcripts in AIDP/CIDP.
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References
Ali ZPM, Ghafouri-Fard S, Komaki A, Mazdeh M, Taheri M, Eftekharian MM (2020a) Assessment of IL-38 levels in patients with acquired immune-mediated polyneuropathies. J Mol Neurosci 70:1385–1388
Ali ZPM, Taheri M, Sangsefidi S, Arsang-Jang S, Mazdeh M, Zamani A, Ghafouri-Fard S, Eftekharian MM (2020b) Evaluation of expression of STAT genes in immune-mediated polyneuropathies. J Mol Neurosci 70:945–952
Aune TM, Spurlock CF 3rd (2016) Long non-coding RNAs in innate and adaptive immunity. Virus Res 212:146–160
Comi C (2011) Fas-mediated T-cell apoptosis in chronic inflammatory demyelinating polyneuropathy. J Peripher Nerv Syst 16(Suppl 1):45–47
Comi C, Osio M, Ferretti M, Mesturini R, Cappellano G, Chiocchetti A, Carecchio M, Nascimbene C, Varrasi C, Cantello R, Mariani C, Monaco F, Dianzani U (2009) Defective Fas-mediated T-cell apoptosis predicts acute onset CIDP. J Peripher Nerv Syst 14:101–106
Dalakas MC (2015) Pathogenesis of immune-mediated neuropathies. Biochim Biophys Acta 1852:658–666
Dimachkie MM, Barohn RJ (2013) Guillain-Barré syndrome and variants. Neurol Clin 31:491–510
Fang P, Xiang L, Chen W, Li S, Huang S, Li J, Zhuge L, Jin L, Feng W, CHEN Y (2019) LncRNA GAS5 enhanced the killing effect of NK cell on liver cancer through regulating miR-544/RUNX3. Innate Immun 25:99–109
Gast M, Rauch BH, Haghikia A, Nakagawa S, Haas J, Stroux A, Schmidt D, Schumann P, Weiss S, Jensen L, Kratzer A, Kraenkel N, Müller C, Börnigen D, Hirose T, Blankenberg S, Escher F, Kühl AA, Kuss AW, Meder B, Landmesser U, Zeller T, Poller W (2019) Long noncoding RNA NEAT1 modulates immune cell functions and is suppressed in early onset myocardial infarction patients. Cardiovasc Res 115:1886–1906
Hadjicharalambous MR, Lindsay MA (2019) Long non-coding RNAs and the innate immune response. Non-Coding RNA 5:34
Islam Z, Jahan I, Ahammad RU, Shahnaij M, Nahar S, Mohammad QD (2018) FAS promoter polymorphisms and serum sFas level are associated with increased risk of nerve damage in Bangladeshi patients with Guillain-Barré syndrome. PLoS One 13:e0192703
Kino T, Hurt DE, Ichijo T, Nader N, Chrousos GP (2010) Noncoding RNA gas5 is a growth arrest–and starvation-associated repressor of the glucocorticoid receptor. Sci Signal 3:ra8–ra8
Lehmann HC, Meyer Zu Horste G, Kieseier BC, Hartung H-P (2009) Pathogenesis and treatment of immune-mediated neuropathies. Ther Adv Neurol Disord 2:261–281
Moharamoghli M, Hassan-Zadeh V, Dolatshahi E, Alizadeh Z, Farazmand A (2019) The expression of GAS5, THRIL, and RMRP lncRNAs is increased in T cells of patients with rheumatoid arthritis. Clin Rheumatol 38:3073–3080
Mourtada-Maarabouni M, Hedge VL, Kirkham L, Farzaneh F, Williams GT (2008) Growth arrest in human T-cells is controlled by the non-coding RNA growth-arrest-specific transcript 5 (GAS5). J Cell Sci 121:939–946
Neurology AAO (1991) Research criteria for diagnosis of chronic inflammatory demyelinating polyneuropathy (CIDP): report from an ad hoc subcommittee of the American Academy of Neurology AIDS task force. Neurology 41:617–618
Rezaei S, Ghafouri-Fard S, Komaki A, Mazdeh M, Taheri M, Eftekharian MM (2020) Increased levels of IL-34 in acquired immune-mediated neuropathies. J Mol Neurosci. https://doi.org/10.1007/s12031-020-01634-4
Sangsefidi S, Ghafouri-Fard S, Komaki A, Mazdeh M, Taheri M, Eftekharian MM (2020) High levels of Il-19 in patients with chronic inflammatory demyelinating polyneuropathy. J Mol Neurosci. https://doi.org/10.1007/s12031-020-01602-y
Schmidt B, Stoll G, Hartung HP, Heininger K, Schäfer B, Toyka KV (1990) Macrophages but not Schwann cells express Ia antigen in experimental autoimmune neuritis. Ann Neurol 28:70–77
Sehgal L, Mathur R, Braun FK, Wise JF, Berkova Z, Neelapu S, Kwak LW, Samaniego F (2014) FAS-antisense 1 lncRNA and production of soluble versus membrane FAS in B-cell lymphoma. Leukemia 28:2376–2387
Uchida S, Adams JC (2019) Physiological roles of non-coding RNAs. American Physiological Society Bethesda, MD
Van Den Bergh PY, Hadden RD, Bouche P, Cornblath DR, Hahn A, Illa I, Koski CL, Léger JM, Nobile-Orazio E, Pollard J, Sommer C, Van Doorn PA, Van Schaik IN (2010) European Federation of Neurological Societies/peripheral nerve society guideline on management of chronic inflammatory demyelinating polyradiculoneuropathy: report of a joint task force of the European Federation of Neurological Societies and the peripheral nerve society - first revision. Eur J Neurol 17:356–363
Van Der Meché F, Van Doorn P, Meulstee J, Jennekens F (2001) Diagnostic and classification criteria for the Guillain-Barré syndrome. Eur Neurol 45:133–139
Wang H, Liao S, Li H, Chen Y, Yu J (2019) Long non-coding RNA TUG1 sponges Mir-145a-5p to regulate microglial polarization after oxygen-glucose deprivation. Front Mol Neurosci 12:215–215
Xu Y, Deng W, Zhang W (2018) Long non-coding RNA TUG1 protects renal tubular epithelial cells against injury induced by lipopolysaccharide via regulating microRNA-223. Biomed Pharmacother 104:509–519
Zhang H, Li H, Ge A, Guo E, Liu S, Zhang L (2018) Long non-coding RNA TUG1 inhibits apoptosis and inflammatory response in LPS-treated H9c2 cells by down-regulation of miR-29b. Biomed Pharmacother 101:663–669
Zhang P, Cao L, Zhou R, Yang X, Wu M (2019) The lncRNA Neat1 promotes activation of inflammasomes in macrophages. Nat Commun 10:1–17
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The current study was supported by a grant from Shahid Beheshti University of Medical Sciences School of Medicine (grant number 17697).
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Sadeghpour, S., Ghafouri-Fard, S., Mazdeh, M. et al. Over-Expression of Immune-Related lncRNAs in Inflammatory Demyelinating Polyradiculoneuropathies. J Mol Neurosci 71, 991–998 (2021). https://doi.org/10.1007/s12031-020-01721-6
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DOI: https://doi.org/10.1007/s12031-020-01721-6