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Pulsed Electromagnetic Field Protects Against Brain Injury After Intracerebral Hemorrhage: Involvement of Anti-Inflammatory Processes and Hematoma Clearance via CD36

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Abstract

Intracerebral hemorrhage causes high mortality and morbidity, but its therapy methods are limited. In the present study, pulsed electromagnetic field (PEMF) was demonstrated to have beneficial effects on an intracerebral hemorrhage (ICH) model. This study explored the effects and underlying mechanisms of PEMF in a mouse model of ICH and cultured BV2 cells. PEMF was applied 4 hours after collagenase-induced ICH at day 0 and 4 hours per day for seven consecutive days. The expression levels of proinflammatory factors were assessed by ELISA kits and western blotting. Hematoma volume was measured by histological analysis. The effects of PEMF on phagocytosis of the erythrocytes were observed in cultured BV2 cells and ICH mouse models. Seven days after ICH, the hematoma volume was significantly reduced in PEMF-treated animals compared to nontreated mice. We found that PEMF decreased the hematoma volume and the expression levels of proinflammatory factors after ICH. Moreover, PEMF enhanced the erythrophagocytosis of microglia via CD36. Furthermore, we found that downregulation CD36 with Genistein blocked the effects of PEMF-induced hematoma clearance and anti-inflammations effects. Thus, the PEMF-mediated promotion of neurological functions may at least partly involve anti-inflammatory processes and hematoma clearance. These results suggest that PEMF treatment promoted the hematoma clearance and alleviated the inflammation after ICH.

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Funding

This study was supported by the Natural Science Foundation of Shaanxi Province, China (no. 2021JQ-344, no. 2020JQ-231); the National Natural Science Foundation of China (no. 82171363, no. 82171321, no. 81901186); and Youth Nova Program of Shaanxi Province, China (no. 2021KJXX-19).

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Author notes

  1. Yuefan Yang, Pan Wang and Anlai Liu have contributed equally to this work and share first authorship.

Authors and Affiliations

  1. Department of Biomedical Engineering, Fourth Military Medical University, Xi’an, People’s Republic of China

    Yuefan Yang, Pan Wang, Zedong Yan & Erping Luo

  2. Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi’an, People’s Republic of China

    Yuefan Yang, Xiuquan Wu, Shuhui Dai, Jialiang Wei, Zhuoyuan Zhang & Peng Luo

  3. Department of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi’an, People’s Republic of China

    Xin Li

  4. Institute of Medical Research, Northwestern Polytechnical University, Xi’an, People’s Republic of China

    Pan Wang

  5. Shaanxi University of Chinese Medicine, Xianyang, People’s Republic of China

    Anlai Liu

  6. College of Life Science, Northwest University, Xi’an, People’s Republic of China

    Zhuoyuan Zhang

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  1. Yuefan Yang
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Contributions

Yuefan Yang designed, performed, and analyzed most figures. Pan Wang and Anlai Liu performed the behavior test. Shuhui Dai, Jialiang Wei, and Zhuoyuan Zhang provided studying materials and technical support. Zedong Yan and Xiuquan Wu performed the statistics work. Xin Li, Peng Luo, and Erping Luo conceived and coordinated the study and wrote the paper. The authors read and approved the final manuscript.

Corresponding authors

Correspondence to Xin Li, Peng Luo or Erping Luo.

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Yang, Y., Wang, P., Liu, A. et al. Pulsed Electromagnetic Field Protects Against Brain Injury After Intracerebral Hemorrhage: Involvement of Anti-Inflammatory Processes and Hematoma Clearance via CD36. J Mol Neurosci 72, 2150–2161 (2022). https://doi.org/10.1007/s12031-022-02063-1

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