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Bioprospecting Sulfuric Acid Assisted Hydrothermal Pretreatment of Sugarcane Bagasse and Microbial Community Structure for Methane Production

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Abstract

The complex chemical and structural arrangement of lignocellulosic biomass demand a pretreatment step to dismantle its coherent nature. The current study was designed to study H2SO4 impregnation before hydrothermal pretreatment (HP) of sugarcane bagasse for biogas production using response surface methodology based on defining factors: temperature (160, 180, 200 °C), time (5, 12, 19 min), and H2SO4 concentration (1, 2, 3% w/v). The H2SO4 impregnation had a paramount impact in combination with temperature and treatment time parameters. The lowest solid yield 12.3% was recorded in pretreatment run A-HSO (200 °C, 19 min, 3% H2SO4), up to 100% xylan removal was noted in four pretreatment runs (A-HSO; 200 °C, 19 min, 3% H2SO4, B-HSO; 200 °C, 19 min, 1% H2SO4, C-HSO; 200 °C, 5 min, 3% H2SO4, and O-HSO; 213.6 °C, 12 min, 2% H2SO4), maximum klason lignin increase in insoluble solid fraction (ISF) verified was 358.85% in pretreatment run O-HSO at 213.6 °C, while glucan content reduction (33.18–74.14%) was also observed in pretreatment runs A-HSO, C-HSO, and O-HSO. Results verified maximum methane recovery of 160.16 NmL (g TVS)−1 in pretreatment run I-SHO (180 °C, 12 min, 2% H2SO4). Microbial community analysis observed the predominance of Flavobacterium and Desulfosporosinus genera of Bacterial domain and Methanolinea of Archaea domain. H2SO4 impregnation before HP though not suitable for methane production from ISF could be employed in a biorefinery perspective to obtain valuables (chemicals and fuels) due to the production of organic acids rich liquid stream and lignin-rich solid fraction.

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Abbreviations

APHA:

American Public Health Association

BMP:

Biomethane potential

CCD:

Central composite design

COD:

Chemical oxygen demand

DM:

Dry matter

GAIN:

Global Agricultural Information Network

HP:

Hydrothermal pretreatment

HPLC:

High-performance liquid chromatography

NCBI:

National Center for Biotechnology Information

OTU:

Operational taxonomic unit

PCR:

Polymerase chain reaction

RDP:

Ribosomal Database Project

SB:

Sugarcane bagasse

SEM:

Scanning electron microscopy

TCD:

Thermal conductivity detector

TS:

Total solids

TVS:

Total volatile solids

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Funding

This research was funded by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Ministry of Education, Brazil, under the grant ID 23699920852.

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Authors and Affiliations

  1. Biofuels Institute, School of Environment and Safety Engineering, Jiangsu University, Zhenjiang, Jiangsu, People’s Republic of China

    Fiaz Ahmad

  2. Department of Hydraulics and Sanitation, School of Engineering of São Carlos, University of São Paulo, Av. Trabalhador São Carlense, 400, São Carlos, SP, 13566-590, Brazil

    Fiaz Ahmad, Vanessa Silva, Isabel Kimiko Sakamoto & Maria Bernadete Amancio Varesche

  3. Department of Chemical Engineering, Federal University of São Carlos, Rod. Washington Luis, km 235, São Carlos, SP, 13565-905, Brazil

    Jianzhong Sun & Edson Luiz Silva

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Contributions

FA, ELS, and MBAV conceived and designed the study. FA conducted the experiments and wrote the manuscript. VS helped in conducting BMP experiments. IKS helped in molecular biology experiments. MBAV, IKS, and JS proofread the manuscript. All authors provided feedback on the manuscript.

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Correspondence to Fiaz Ahmad or Maria Bernadete Amancio Varesche.

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Ahmad, F., Silva, V., Sakamoto, I.K. et al. Bioprospecting Sulfuric Acid Assisted Hydrothermal Pretreatment of Sugarcane Bagasse and Microbial Community Structure for Methane Production. Bioenerg. Res. 15, 631–649 (2022). https://doi.org/10.1007/s12155-021-10268-2

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