Abstract
The use of fruit residues from domestic consumption for energy purposes is a perspective of innovation in the search for a more sustainable fuel, due to the low cost of this biomass, combined with its large amounts of generation, which has sugars readily available in its composition and does not require process pretreatment hydrolysis complexes of the material. This work evaluated residues of melon, pineapple, banana, apple, and mango, to compose a mixture of fruit waste (MFW), simulating a household waste for bioethanol production by a non-conventional yeast Wickerhamomyces sp. UFFS-CE-3.1.2. Sugar extraction was optimized by experimental design (MFW: 12% g dry mass v−1; 25 °C; 5 min) and resulted in the liberation of 36.32 ± 0.72 g L−1 fermentable sugar. MFW was used for alcoholic fermentation with and without nitrogen supplementation (urea and yeast extract). The results demonstrated that it is not necessary for the supplementation, making the process more economically viable. The maximum ethanol productivity (2.50±0.06 g L−1 h−1) was achieved in 9 h of the operation. The MFW extracted is an alternative for the bioethanol process as low cost and straightforward, adapted for different fruit residues, and used as a unique or diluent medium in the biorefinery context. Moreover, non-conventional yeast demonstrated the more new one in this study that explores the potential of yeast recently isolated.
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References
Scapini T, Favaretto DPC, Camargo AF, Czapela FF, Bonatto C, Zanivan J, Dalastra C, Klanovicz N, Souza T, Muller C, Fongaro G, Treichel H (2019) Bioethanol from fruit. In: Treichel H, Alves SL Jr, Fongaro G, Muller C (eds) Ethanol as a Green Alternative Fuel: Insight and Perspectives, 1st edn. Nova Science Publishers, New York, pp 139–176
Liakou V, Pateraki C, Palaiogeorgou A-M, Kopsahelis N, Machado de Castro A, Guimarães Freire DM, Nychas G-JE, Papanikolaou S, Koutinas A (2018) Valorisation of fruit and vegetable waste from open markets for the production of 2,3-butanediol. Food Bioprod Process 108:27–36. https://doi.org/10.1016/j.fbp.2017.10.004
Sarkar D, Prajapati S, Poddar K, Sarkar A (2019) Production of ethanol by Enterobacter sp. EtK3 during fruit waste biotransformation. Int Biodeterior Biodegradation 145:1–7. https://doi.org/10.1016/j.ibiod.2019.104795
Sagar NA, Pareek S, Sharma S, Yahia EM, Lobo MG (2018) Fruit and vegetable waste: bioactive compounds, their extraction, and possible utilization. Compr Rev Food Sci Food Saf 17:512–531. https://doi.org/10.1111/1541-4337.12330
FAO (2019) The stage of food and agriculture – moving forward on food loss and waste reduction. In: FOOD Agric. Organ. UNITED NATIONS
Kist BB, Santos CE dos, Carvalho C de, Beling RR (2018) Brazilian horti and fruit yearbook. In: Gazeta. http://www.abcsem.com.br/upload/arquivos/HortiFruti_2019_DUPLA.pdf
Shalini R, Gupta DK (2010) Utilization of pomace from apple processing industries: a review. J Food Sci Technol 47:365–371. https://doi.org/10.1007/s13197-010-0061-x
Pathak PD, Mandavgane SA, Kulkarni BD (2017) Fruit peel waste: characterization and its potential uses. Curr Sci 113:444–454
Lima FDC, Simões AJA, Vieira IMM, Silva DP, Ruzene DS (2018) An overview of applications in pineapple agroindustrial residues. Acta Agric Slov 111:445. https://doi.org/10.14720/aas.2018.111.2.18
Bonatto C, Venturin B, Mayer DA, Bazoti SF, de Oliveira D, Alves SL, Treichel H (2020) Experimental data and modelling of 2G ethanol production by Wickerhamomyces sp. UFFS-CE-3.1.2. Renew Energy 145:2445–2450. https://doi.org/10.1016/j.renene.2019.08.010
Nandal P, Sharma S, Arora A (2020) Bioprospecting non-conventional yeasts for ethanol production from rice straw hydrolysate and their inhibitor tolerance. Renew Energy 147:1694–1703. https://doi.org/10.1016/j.renene.2019.09.067
Zabed HM, Akter S, Yun J, Zhang G, Awad FN, Qi X, Sahu JN (2019) Recent advances in biological pretreatment of microalgae and lignocellulosic biomass for biofuel production. Renew Sust Energ Rev 105:105–128. https://doi.org/10.1016/j.rser.2019.01.048
Bonatto C, Scapini T, Zanivan J, Dalastra C, Bazoti SF, Alves S, Fongaro G, de Oliveira D, Treichel H (2021) Utilization of seawater and wastewater from shrimp production in the fermentation of papaya residues to ethanol. Bioresour Technol 321:124501. https://doi.org/10.1016/j.biortech.2020.124501
Bazoti SF, Golunski S, Pereira Siqueira D, Scapini T, Barrilli ÉT, Alex Mayer D, Barros KO, Rosa CA, Stambuk BU, Alves SL, Valério A, de Oliveira D, Treichel H (2017) Second-generation ethanol from non-detoxified sugarcane hydrolysate by a rotting wood isolated yeast strain. Bioresour Technol 244:582–587. https://doi.org/10.1016/j.biortech.2017.08.007
Ajila CM, Bhat SG, Prasada Rao UJS (2007) Valuable components of raw and ripe peels from two Indian mango varieties. Food Chem 102:1006–1011. https://doi.org/10.1016/j.foodchem.2006.06.036
Moura AGC, de Souza RLA, de Oliveira ENA (2017) Preparation and physicochemical and sensory characterization of melon rind and albedo of crystallized passion fruit. Tecnol e ciência agropecuária 11:77–81
Rodrigues MI, Iemma AF (2014) Experimental design and process optimization, first edn. CRC Press
Miller GL (1959) Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar. Anal Chem 31:426–428. https://doi.org/10.1021/ac60147a030
Tan JS, Phapugrangkul P, Lee CK, Lai Z-W, Abu Bakar MH, Murugan P (2019) Banana frond juice as novel fermentation substrate for bioethanol production by Saccharomyces cerevisiae. Biocatal Agric Biotechnol 21:101293. https://doi.org/10.1016/j.bcab.2019.101293
Shuler MLFK (2002) How Cells Grow. In: Shuler ML, Kargi F (eds) Bioprocess engineering: basic concepts, 2nd edn. Prentice Hall PTR, Upper Saddle River, pp 155–207
Turhan I, Bialka KL, Demirci A, Karhan M (2010) Ethanol production from carob extract by using Saccharomyces cerevisiae. Bioresour Technol 101:5290–5296. https://doi.org/10.1016/j.biortech.2010.01.146
Liu L, Tan S, Horikawa T, Do DD, Nicholson D, Liu J (2017) Water adsorption on carbon - a review. Adv Colloid Interf Sci 250:64–78. https://doi.org/10.1016/j.cis.2017.10.002
Silva GP, De Araújo EF, Silva DO, Guimarães WV (2005) Ethanolic fermentation of sucrose, sugarcane juice and molasses by Escherichia coli strain KO11 and Klebsiella oxytoca strain P2. Braz J Microbiol 36:395–404
Choi IS, Lee YG, Khanal SK, Park BJ, Bae H-J (2015) A low-energy, cost-effective approach to fruit and citrus peel waste processing for bioethanol production. Appl Energy 140:65–74. https://doi.org/10.1016/j.apenergy.2014.11.070
Abdullah SSS, Shirai Y, Bahrin EK, Hassan MA (2015) Fresh oil palm frond juice as a renewable, non-food, non-cellulosic and complete medium for direct bioethanol production. Ind Crop Prod 63:357–361. https://doi.org/10.1016/j.indcrop.2014.10.006
Zhao X, Procopio S, Becker T (2015) Flavor impacts of glycerol in the processing of yeast fermented beverages: a review. J Food Sci Technol 52:7588–7598. https://doi.org/10.1007/s13197-015-1977-y
Li Z, Wang D, Shi Y-C (2017) Effects of nitrogen source on ethanol production in very high gravity fermentation of corn starch. J Taiwan Inst Chem Eng 70:229–235. https://doi.org/10.1016/j.jtice.2016.10.055
Yue G, Yu J, Zhang X, Tan T (2012) The influence of nitrogen sources on ethanol production by yeast from concentrated sweet sorghum juice. Biomass Bioenergy 39:48–52. https://doi.org/10.1016/j.biombioe.2010.08.041
Kim YG, Lyu J, Kim MK, Lee K-G (2015) Effect of citrulline, urea, ethanol, and urease on the formation of ethyl carbamate in soybean paste model system. Food Chem 189:74–79. https://doi.org/10.1016/j.foodchem.2015.02.012
Funding
This research was financially supported by the UFFS (Universidade Federal da Fronteira Sul - Federal University of Fronteira Sul), FAPERGS, CAPES, and CNPq for the scholarship and the financial support.
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Jessica Zanivan: conceptualization, investigation, data curation, writing—original draft
Charline Bonatto: conceptualization, methodology, writing—review and editing, visualization
Thamarys Scapini: conceptualization, methodology, writing—review and editing
Caroline Dalastra: methodology
Suzana F. Bazoti: methodology
Sérgio Luiz Alves Júnior: resources, writing—review and editing
Gislaine Fongaro: supervision, writing—review and editing
Helen Treichel: resources, supervision, writing—review and editing
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Zanivan, J., Bonatto, C., Scapini, T. et al. Evaluation of Bioethanol Production from a Mixed Fruit Waste by Wickerhamomyces sp. UFFS-CE-3.1.2. Bioenerg. Res. 15, 175–182 (2022). https://doi.org/10.1007/s12155-021-10273-5
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DOI: https://doi.org/10.1007/s12155-021-10273-5