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Comparison of two bacterial DNA extraction methods from non-polluted and polluted soils

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Abstract

DNA extraction from soil samples is a critical step for molecular biology analyses. The present study compared the efficiency of two DNA isolation methods from non-polluted and polluted soils with or without the presence of a plant. Both applied methods used chemical and physical lyses, but method 1 had an additional physical disruption. The main difference between these two methods was the humic acid purification technique as it was carried out during cell lysis for method 1 and after cell lysis for method 2. Samples were assessed on the basis of their yield and DNA purity as well as their bacterial quantity and diversity. Based on our results, method 1 proved to be more effective at removing protein and RNA, whereas method 2 proved to be more effective at removing humic acids. Although no differences were obtained in terms of the DNA yield, both the bacterial quantity and community structure were affected by the method used. Method 1 allowed for the recovery of more information than method 2, and polluted soil was more sensitive to the DNA extraction procedure. We recommend carefully selecting the DNA extraction method, especially when soil is disturbed.

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Acknowledgements

We thank «Communauté d’agglomérations Porte de France—Thionville» for his financial support.

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Authors and Affiliations

  1. Laboratoire Interdisciplinaire des Environnements Continentaux, UMR 7360, Université de Lorraine, Thionville-Yutz, France

    Mélanie Mazziotti, Sonia Henry, Philippe Laval-Gilly & Jaïro Falla

  2. CNRS, Laboratoire Interdisciplinaire des Environnements Continentaux, UMR 7360, CNRS, Thionville-Yutz, France

    Mélanie Mazziotti, Sonia Henry, Philippe Laval-Gilly & Jaïro Falla

  3. Université de Lorraine, IUT Thionville-Yutz, Thionville-Yutz, France

    Mélanie Mazziotti, Sonia Henry, Philippe Laval-Gilly, Antoine Bonnefoy & Jaïro Falla

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  1. Mélanie Mazziotti
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  2. Sonia Henry
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  3. Philippe Laval-Gilly
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Correspondence to Mélanie Mazziotti or Sonia Henry.

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Mazziotti, M., Henry, S., Laval-Gilly, P. et al. Comparison of two bacterial DNA extraction methods from non-polluted and polluted soils. Folia Microbiol 63, 85–92 (2018). https://doi.org/10.1007/s12223-017-0530-y

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  • DOI: https://doi.org/10.1007/s12223-017-0530-y

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