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Entry into midgut epithelial cells is a key step in the selection of genotypes in a nucleopolyhedrovirus

  • Published:
Virologica Sinica

Abstract

An isolate of the Spodoptera frugiperda multiple nucleopolyhedrovirus comprises a stable proportion of deletion genotypes (e.g., SfNIC-C), that lack pif1 and pif2 rendering them noninfectious per os, and that survive by complementation with a complete genotype (SfNIC-B) in coinfected cells. To determine whether selection for particular ratios of complete and deletion genotypes occurs mainly during the establishment of the primary infection in insect midgut cells or during subsequent systemic infection, we examined genotype frequencies in insects that fed on OBs comprising different co-occluded mixtures of genotypes. Dramatic changes in genotype frequencies were observed between the OB inoculum and budded virus (BV) samples taken from larvae inoculated with OBs comprising 10% SfNIC-B + 90% SfNIC-C indicating that a marked reduction of SfNIC-C genotype had occurred in the insect midgut due to the immediate elimination of all OBs that originated from cells that had been infected only by SfNIC-C. In contrast, immediate changes were not observed in OBs comprising mixtures of 50% SfNIC-B + 50% SfNIC-C or those comprising 10% SfNIC-B + 90% SfNIC-C as most of the OBs in these mixtures originated from cells that had been infected by both genotypes. Subsequent changes in genotypic frequencies during five days of systemic infection were fairly small in magnitude for all genotypic mixtures. We conclude that the prevalence of defective genotypes in the SfNIC population is likely determined by a balance between host selection against OBs produced in cells infected by SfNIC-C alone and within-host selection for fast-replicating deletion genotypes. The strength of intra-host selection is likely modulated by changes in MOI during the infection period.

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Authors and Affiliations

  1. Laboratorio de Entomología Agrícola y Patología de Insectos, Departamento de Producción Agraria, Universidad Pública de Navarra, 31006, Pamplona, Spain

    Gabriel Clavijo, Delia Muñoz & Primitivo Caballero

  2. CSIC, Gobierno de Navarra, Instituto de Agrobiotecnología, Mutilva Baja, Spain

    Gabriel Clavijo, Delia Muñoz & Primitivo Caballero

  3. Instituto de Ecología A.C., Xalapa, Veracruz, Mexico

    Trevor Williams

  4. Laboratoire de Gènie de l’Environnement Industriel, Ecole de Mines d’Alès, Alès, France

    Miguel López-Ferber

Authors
  1. Gabriel Clavijo
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  2. Trevor Williams
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  3. Delia Muñoz
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  4. Miguel López-Ferber
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  5. Primitivo Caballero
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Corresponding author

Correspondence to Primitivo Caballero.

Additional information

Foundation items: This study was funded by MEC projects numbers AGL2005-07909-CO3-01 and AGL2008-05456-CO3-01/AGR

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Clavijo, G., Williams, T., Muñoz, D. et al. Entry into midgut epithelial cells is a key step in the selection of genotypes in a nucleopolyhedrovirus. Virol. Sin. 24, 350–358 (2009). https://doi.org/10.1007/s12250-009-3048-x

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  • DOI: https://doi.org/10.1007/s12250-009-3048-x

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