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Development of multiple elisas for the detection of antibodies against classical swine fever virus in pig sera

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Virologica Sinica

Abstract

The major immunogenic proteins (Erns, E2 and NS3) of classical swine fever virus (CSFV) (Shimen strain) were expressed in E. coli and purified by affinity chromatography. The recombinant antigens were applied to develop multiple enzyme-linked immunosorbent assays (ELISAs) for the detection of specific antibodies in pig sera. Optimum cut-off values were determined by receiver operating characteristic (ROC) analysis after testing 201 sera of vaccinated pigs and 64 negative sera of unvaccinated piglets. The multiple ELISAs were validated with 265 pig sera yielding high sensitivity and specificity in comparison with the virus neutralization results. The results demonstrated that multiple ELISAs can be a valuable tool for the detection of CSFV infection and serological surveys in CSFV-free countries or for the evaluation of the antibody responses in pigs induced by a live attenuated C-strain vaccination.

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Authors and Affiliations

  1. State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, 430072, China

    Zhen-hua Yang, Ling Li & Zi-shu Pan

Authors
  1. Zhen-hua Yang
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  2. Ling Li
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  3. Zi-shu Pan
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Correspondence to Zi-shu Pan.

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Foundation items: the National Natural Science Foundation of China (30771597); the Key Project of Science and Technology of Wuhan (200720422141).

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Yang, Zh., Li, L. & Pan, Zs. Development of multiple elisas for the detection of antibodies against classical swine fever virus in pig sera. Virol. Sin. 27, 48–56 (2012). https://doi.org/10.1007/s12250-012-3227-z

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  • DOI: https://doi.org/10.1007/s12250-012-3227-z

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