Abstract
Suitable animal models for human immunodeficiency virus type 1 (HIV-1) infection are important for elucidating viral pathogenesis and evaluating antiviral strategies in vivo. The B-NSG (NOD-PrkdcscidIl2rgtm1/Bcge) mice that have severe immune defect phenotype are examined for the suitability of such a model in this study. Human peripheral blood mononuclear cells (PBMCs) were engrafted into B-NSG mice via mouse tail vein injection, and the repopulated human T-lymphocytes were observed at as early as 3-weeks post-transplantation in mouse peripheral blood and several tissues. The humanized mice could be infected by HIV-1, and the infection recapitulated features of T-lymphocyte dynamic observed in HIV-1 infected humans, meanwhile the administration of combination antiretroviral therapy (cART) suppressed viral replication and restored T lymphocyte abnormalities. The establishment of HIV-1 infected humanized B-NSG mice not only provides a model to study virus and T cell interplays, but also can be a useful tool to evaluate antiviral strategies.
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Acknowledgements
This work was supported by Grants to JHW from the National Grant Program on Key Infectious Disease (2018ZX10301101-003-002), the Natural Science Foundation of China (NSFC, 81572001, 81873965), the key project from Chinese Academy of Sciences (QYZDB-SSW-SMC059), and grant to WWS from NSFC (31800152). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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TJF, LS and WWS conceived the experiments, designed the experimental flow and performed the experiments. TJF performed statistical analyses. TJF, WWS and JHW wrote the manuscript. TJF, XGY and XJ reviewed the manuscript. WWS and JHW supervised the project. All authors read and approved the final manuscript.
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Animal and Human Rights Statement
All procedures were conducted in compliance with a protocol approved by the Institutional Animal Care and Use Committee at Institut Pasteur of Shanghai. All experiments were performed in accordance with relevant guidelines and regulations.
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Fan, TJ., Sun, L., Yang, XG. et al. The Establishment of an In Vivo HIV-1 Infection Model in Humanized B-NSG Mice. Virol. Sin. 35, 417–425 (2020). https://doi.org/10.1007/s12250-019-00181-6
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DOI: https://doi.org/10.1007/s12250-019-00181-6