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Development of an MCA-Based Real Time RT-qPCR Assay for the Simultaneous Detection and Differentiation of Duck Hepatitis A Virus Types 1 and 3

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Virologica Sinica

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References

  • Cha SY, Roh JH, Kang M, Kim B, Jang HK (2013) Isolation and characterization of a low pathogenic duck hepatitis A virus 3 from South Korea. Vet Microbiol 162:254–258

    Article  CAS  Google Scholar 

  • Chen LL, Xu Q, Zhang RH, Yang L, Li JX, Xie ZJ, Zhu YL, Jiang SJ, Si XK (2013) Improved duplex RT-PCR assay for differential diagnosis of mixed infection of duck hepatitis A virus type 1 and type 3 in ducklings. J Virol Methods 192:12–17

    Article  CAS  Google Scholar 

  • Chen X, Chen Y, Liu C, Li X, Liu H, Yin X, Bai X, Ge M, Chen H, Liu M, Du Y, Fan G, Zhang Y (2019) Improved one-tube RT-PCR method for simultaneous detection and genotyping of duck hepatitis A virus subtypes 1 and 3. PLoS ONE 14:e0219750

    Article  CAS  Google Scholar 

  • Ding C, Zhang D (2007) Molecular analysis of duck hepatitis virus type 1. Virology 361:9–17

    Article  CAS  Google Scholar 

  • Doan HT, Le XT, Do RT, Hoang CT, Nguyen KT, Le TH (2016) Molecular genotyping of duck hepatitis A viruses (DHAV) in Vietnam. J Infect Dev Ctries 10:988–995

    Article  CAS  Google Scholar 

  • Gan Y, Huang C, Wang X, Chen S, Li J (2014) Epidemiological investigation of duck hepatitis A virus (DHAV) isolated from Sichuan Basin by RT-PCR disclose the existence of mixed infection and the feasibility of DHAV-A evolved from C80 strain. Pak Vet J 34:356–360

    Google Scholar 

  • Hu Q, Zhu D, Ma G, Cheng A, Wang M, Chen S, Jia R, Liu M, Sun K, Yang Q (2016) A one-step duplex rRT-PCR assay for the simultaneous detection of duck hepatitis A virus genotypes 1 and 3. J Virol Methods 236:207–214

    Article  CAS  Google Scholar 

  • Huang Q, Yue H, Zhang B, Nie P, Tang C (2012) Development of a real-time quantitative PCR for detecting duck hepatitis a virus genotype C. J Clin Microbiol 50:3318–3323

    Article  CAS  Google Scholar 

  • Kim MC, Kwon YK, Joh SJ, Lindberg AM, Kwon JH, Kim JH, Kim SJ (2006) Molecular analysis of duck hepatitis virus type 1 reveals a novel lineage close to the genus Parechovirus in the family Picornaviridae. J Gen Virol 87:3307–3316

    Article  CAS  Google Scholar 

  • Kim MC, Kwon YK, Joh SJ, Kim SJ, Tolf C, Kim JH, Sung HW, Lindberg AM, Kwon JH (2007) Recent Korean isolates of duck hepatitis virus reveal the presence of a new geno- and serotype when compared to duck hepatitis virus type 1 type strains. Arch Virol 152:2059–2072

    Article  CAS  Google Scholar 

  • Lin SL, Cong RC, Zhang RH, Chen JH, Xia LL, Xie ZJ, Wang Y, Zhu YL, Jiang SJ (2016) Circulation and in vivo distribution of duck hepatitis A virus types 1 and 3 in infected ducklings. Arch Virol 161:405–416

    Article  CAS  Google Scholar 

  • Liu M, Fanyi M, Li XJ, Zhang Z, Liu S, Zhang Y (2011) Goose haemorrhagic hepatitis caused by a new subtype duck hepatitis type 1 virus. Vet Microbiol 152:280–283

    Article  Google Scholar 

  • Soliman M, Alfajaro MM, Lee MH, Jeong YJ, Kim DS, Son KY, Kwon J, Choi JS, Lim JS, Choi JS, Lee TU, Cho KO, Kang MI (2015) The prevalence of duck hepatitis A virus types 1 and 3 on Korean duck farms. Arch Virol 160:493–498

    Article  CAS  Google Scholar 

  • Toth TE (1969) Studies of an agent causing mortality among ducklings immune to duck virus hepatitis. Avian Dis 13:834–846

    Article  CAS  Google Scholar 

  • Tseng CH, Knowles NJ, Tsai HJ (2007) Molecular analysis of duck hepatitis virus type 1 indicates that it should be assigned to a new genus. Virus Res 123:190–203

    Article  CAS  Google Scholar 

  • Tseng CH, Tsai HJ (2007) Molecular characterization of a new serotype of duck hepatitis virus. Virus Res 126:19–31

    Article  CAS  Google Scholar 

  • Wen X, Zhu D, Cheng A, Wang M, Chen S, Jia R, Liu M, Sun K, Zhao X, Yang Q, Wu Y, Chen X (2018) Molecular epidemiology of duck hepatitis a virus types 1 and 3 in China, 2010–2015. Transbound Emerg Dis 65:10–15

    Article  CAS  Google Scholar 

  • Yang M, Cheng A, Wang M, Xing H (2008) Development and application of a one-step real-time Taqman RT-PCR assay for detection of Duck hepatitis virus type1. J Virol Methods 153:55–60

    Article  CAS  Google Scholar 

  • Yugo DM, Hauck R, Shivaprasad HL, Meng XJ (2016) Hepatitis virus infections in poultry. Avian Dis 60:576–588

    Article  Google Scholar 

  • Zhang R, Xia L, Chen J, Gong Y, Zhang L, Li P, Liu H, Xie Z, Jiang S (2017) Molecular epidemiology and genetic diversity of duck hepatitis A virus type 3 in Shandong province of China, 2012–2014. Acta Virol 61:463–472

    Article  CAS  Google Scholar 

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Acknowledgements

This work was supported by the National Natural Science Foundation of China Grants 31300141 (to CCM) and Shanghai Key Laboratory of Veterinary Biotechnology Grants klab201702 (to CCM).

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Authors and Affiliations

  1. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China

    Chunchun Meng, Yunxiu Huang, Zaib Ur Rehman, Wen Hu, Chuanfeng Li, Ruiying Liang, Zongyan Chen, Kaijie Song & Guangqing Liu

  2. College of Animal Science and Technology, Guangxi University, Nanning, 530005, China

    Yunxiu Huang & Tianchao Wei

Authors
  1. Chunchun Meng
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  2. Yunxiu Huang
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  3. Zaib Ur Rehman
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  4. Wen Hu
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  5. Chuanfeng Li
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  6. Ruiying Liang
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  7. Zongyan Chen
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  8. Kaijie Song
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  9. Tianchao Wei
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  10. Guangqing Liu
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Corresponding author

Correspondence to Guangqing Liu.

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Conflict of interest

The authors declare that they have no conflict of interest.

Animal and Human Rights Statement

This research was approved by the Ethics Committee of Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences. The international guidelines for the care and use of animals have been followed.

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Meng, C., Huang, Y., Rehman, Z.U. et al. Development of an MCA-Based Real Time RT-qPCR Assay for the Simultaneous Detection and Differentiation of Duck Hepatitis A Virus Types 1 and 3. Virol. Sin. 35, 666–669 (2020). https://doi.org/10.1007/s12250-020-00211-8

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  • DOI: https://doi.org/10.1007/s12250-020-00211-8

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