Abstract
Esophageal cancer (EC) is known as one of the most prevalent gastrointestinal cancers, and results in the seventh highest number of cancer-relevant deaths. Long non-coding RNAs (lncRNAs) have substantial roles in several biological processes. LncRNA human urothelial carcinoma associated 1 (UCA1) is announced to be enhanced in multiple types of human cancers. This survey was carried out to identify the potential role of the lncRNA-UCA1 in the progression of EC. A case-control investigation was performed on 140 FFPE tissues of EC patients consisting of 70 cancerous tissues and 70 marginal tissues samples. To determine the lncRNA-UCA1 gene expression changes, quantitative reverse-transcription polymerase chain reaction (qRT-PCR) method was utilized. In addition, the associations between the lncRNA-UCA1 gene expression and clinicopathological parameters were assessed. Our findings revealed that the lncRNA-UCA1 was notably up-regulated in EC tissues compared to adjacent normal tissues (P < 0.05). LncRNA-UCA1 expression was substantially correlated to alcohol drinking (P = 0.008) and socioeconomic status (P = 0.001), while shared no correlation with age, hot drinking status and stage (P > 0.05). Our data indicated that the lncRNA-UCA1 play an important role in the progression of EC and may be considered as a candidate gene in the pathogenesis of EC patients.
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References
Zhang Y (2013 14) Epidemiology of esophageal cancer. World J Gastroenterol: WJG 19(34):5598
Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J, Jemal A (2015) Global cancer statistics, 2012. CA Cancer J Clin 65(2):87–108
Ghorbian S, Ardekani AM (2012) Non-invasive detection of esophageal Cancer using genetic changes in circulating cell-free DNA. Avicenna J Med Biotechnol 4(1):3–13
Song Q, Liu H, Wang J, Jia Y, Liu Y, Wang N, Tan B, Guan S, An D, Cheng Y (2014) Dinner-to-bed time and post-dinner walk: new potential independent factors in esophageal cancer development. J Cancer Res Clin Oncol 140(5):817–821
Tang WR, Fang JY, Wu KS, Shi XJ, Luo JY, Lin K (2014) Epidemiological characteristics and prediction of esophageal cancer mortality in China from 1991 to 2012. Asian Pac J Cancer Prev 15(16):6929–6934
Park S, Paeng JC, Kang CH, Cheon GJ, Kang KW, Chung JK, Lee DS (2018) Dual-time point 18 F-FDG PET/CT for the staging of oesophageal cancer: the best diagnostic performance by retention index for N-staging in non-calcified lymph nodes. Eur J Nucl Med Mol Imaging:1–2
Lanzafame M, Bianco G, Terracciano L, Ng C, Piscuoglio S (2018) The role of long non-coding RNAs in hepatocarcinogenesis. Int J Mol Sci 19(3):682
Aalijahan H, Ghorbian S (2018) Long non-coding RNAs and cervical cancer. Exp Mol Pathol
Iyer MK, Niknafs YS, Malik R, Singhal U, Sahu A, Hosono Y, Barrette TR, Prensner JR, Evans JR, Zhao S, Poliakov A (2015) The landscape of long noncoding RNAs in the human transcriptome. Nat Genet 47(3):199–208
Cabili MN, Trapnell C, Goff L, Koziol M, Tazon-Vega B, Regev A, Rinn JL (2011) Integrative annotation of human large intergenic noncoding RNAs reveals global properties and specific subclasses. Genes Dev 2
Martianov I, Ramadass A, Barros AS, Chow N, Akoulitchev A (2007) Repression of the human dihydrofolate reductase gene by a non-coding interfering transcript. Nature 445(7128):666–670
Bayoumi AS, Sayed A, Broskova Z, Teoh JP, Wilson J, Su H, Tang YL, Kim IM (2016) Crosstalk between long noncoding RNAs and microRNAs in health and disease. Int J Mol Sci 17(3):356
Wang KC, Yang YW, Liu B, Sanyal A, Corces-Zimmerman R, Chen Y, Lajoie BR, Protacio A, Flynn RA, Gupta RA, Wysocka J (2011) A long noncoding RNA maintains active chromatin to coordinate homeotic gene expression. Nature. 472(7341):120–124
Ye H, Lin J, Yao X, Li Y, Lin X, Lu H (2018) Overexpression of long non-coding RNA NNT-AS1 correlates with tumor progression and poor prognosis in osteosarcoma. Cell Physiol Biochem 45(5):1904–1914
Xue M, Chen W, Li X (2016) Urothelial cancer associated 1: a long noncoding RNA with a crucial role in cancer. J Cancer Res Clin Oncol 142(7):1407–1419
Liu FT, Dong Q, Gao H, Zhu ZM (2017) The prognostic significance of UCA1 for predicting clinical outcome in patients with digestive system malignancies. Oncotarget 8(25):40620
Huang J, Zhou N, Watabe K, Lu Z, Wu F, Xu M, Mo YY (2015) Long non-coding RNA UCA1 promotes breast tumor growth by suppression of p27 (Kip1). Cell Death Dis 5(1):e1008
Yang YT, Wang YF, Lai JY, Shen SY, Wang F, Kong J, Zhang W, Yang HY (2016) Long non-coding RNA UCA 1 contributes to the progression of oral squamous cell carcinoma by regulating the WNT/β-catenin signaling pathway. Cancer Sci 107(11):1581–1589
Bian Z, Jin L, Zhang J, Yin Y, Quan C, Hu Y, Feng Y, Liu H, Fei B, Mao Y, Zhou L (2016) LncRNA—UCA1 enhances cell proliferation and 5-fluorouracil resistance in colorectal cancer by inhibiting miR-204-5p. Sci Rep 6:23892
Razavi M, Ghorbian S (2019) Original article - up-regulation of long non-coding RNA-PCAT-1 promotes invasion and metastasis in esophageal squamous cell carcinoma. EXCLI 18:422–428
Sadeghpour S, Ghorbian S (2019) Evaluation of the potential clinical prognostic value of lncRNA-BANCR gene in esophageal squamous cell carcinoma. Mol Biol Rep 46:991–995
Qian Y, Liu D, Cao S, Tao Y, Wei D, Li W, Li G, Pan X, Lei D (2017) Upregulation of the long noncoding RNA UCA1 affects the proliferation, invasion, and survival of hypopharyngeal carcinoma. Mol Cancer 16(1):68
Zhao W, Sun C, Cui Z (2017) A long noncoding RNA UCA1 promotes proliferation and predicts poor prognosis in glioma. Clin Transl Oncol 19(6):735–741
Chen P, Wan D, Zheng D, Zheng Q, Wu F, Zhi Q (2016) Long non-coding RNA UCA1 promotes the tumorigenesis in pancreatic cancer. Biomed Pharmacother 83:1220–1226
Wang HM, Lu JH, Chen WY, Gu AQ (2015) Upregulated lncRNA-UCA1 contributes to progression of lung cancer and is closely related to clinical diagnosis as a predictive biomarker in plasma. Int J Clin Exp Med 8(7):11824
Fang Z, Wu L, Wang L, Yang Y, Meng Y, Yang H (2014) Increased expression of the long non-coding RNA UCA1 in tongue squamous cell carcinomas: a possible correlation with cancer metastasis. Oral Surg Oral Med Oral Pathol Oral Radiol 117(1):89–95
Huang J, Zhou N, Watabe K, Lu Z, Wu F, Xu M, Mo YY (2015) Long non-coding RNA UCA1 promotes breast tumor growth by suppression of p27 (Kip1). Cell Death Dis 5(1):e1008
Han Y, Yang YN, Yuan HH, Zhang TT, Sui H, Wei XL, Liu L, Huang P, Zhang WJ, Bai YX (2014) UCA1, a long non-coding RNA up-regulated in colorectal cancer influences cell proliferation, apoptosis and cell cycle distribution. Pathology. 46(5):396–401
Zheng Q, Wu F, Dai WY, Zheng DC, Zheng C, Ye H, Zhou B, Chen JJ, Chen P (2015) Aberrant expression of UCA1 in gastric cancer and its clinical significance. Clin Transl Oncol 17(8):640–646
Wang F, Ying HQ, He BS, Pan YQ, Deng QW, Sun HL, Chen J, Liu X, Wang SK (2015) Upregulated lncRNA-UCA1 contributes to progression of hepatocellular carcinoma through inhibition of miR-216b and activation of FGFR1/ERK signaling pathway. Oncotarget 6(10):7899–7917
Tian Y, Zhang X, Hao Y, Fang Z, He Y (2014) Potential roles of abnormally expressed long noncoding RNA UCA1 and Malat-1 in metastasis of melanoma. Melanoma Res 24(4):335–341
Liu SP, Yang JX, Cao DY, Shen K (2013) Identification of differentially expressed long non-coding RNAs in human ovarian cancer cells with different metastatic potentials. Cancer Biology & Medicine 10(3):138
Li JY, Ma X, Zhang CB (2014) Overexpression of long non-coding RNA UCA1 predicts a poor prognosis in patients with esophageal squamous cell carcinoma. Int J Clin Exp Pathol 7(11):7938
Wang X, Gao Z, Liao J, Shang M, Li X, Yin L, Pu Y, Liu R (2016) lncRNA UCA1 inhibits esophageal squamous-cell carcinoma growth by regulating the Wnt signaling pathway. J Toxic Environ Health A 79(9–10):407–418
Jiao C, Song Z, Chen J, Zhong J, Cai W, Tian S, Chen S, Yi Y, Xiao Y (2016) lncRNA-UCA1 enhances cell proliferation through functioning as a ceRNA of Sox4 in esophageal cancer. Oncol Rep 36(5):2960–2966
Deng F, Zhou K, Cui W, Liu D, Ma Y (2015) Clinicopathological significance of wnt/β-catenin signaling pathway in esophageal squamous cell carcinoma. Int J Clin Exp Pathol 8(3):3045
Acknowledgements
This article was extracted from a M.Sc. thesis (IR.961003) at Ahar Branch Islamic Azad University. We would like to appreciate all the patients and staffs of the Tabriz International Hospital.
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Aalijahan, H., Ghorbian, S. Clinical Application of Long Non-Coding RNA-UCA1 as a Candidate Gene in Progression of Esophageal Cancer. Pathol. Oncol. Res. 26, 1441–1446 (2020). https://doi.org/10.1007/s12253-019-00711-3
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DOI: https://doi.org/10.1007/s12253-019-00711-3