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Schnelle und aussagekräftige Genotoxizitätsbewertung

  • Anwendungen & Produkte
  • Messung von DNA-Strangbrüchen
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Abstract

At present, just a few of the available in vitro genotoxicity tests measure DNA strand breaks and their repair in cells. The comet assay as the considered “gold standard” is still not standardized and does not allow full automation so far. In contrast, most steps of the fluorimetic analysis of alkaline DNA unwinding (FADU) assay could be automated which allows precise dispensing of solutions and control of temperature and process time during the assay. This progress has led to improved reproducibility and throughput, easier handling, and enhanced sensitivity and speed.

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Authors and Affiliations

  1. Institut Für Molekulare Toxikologie, Universität Konstanz, Konstanz, Deutschland

    Maria Moreno-Villanueva

  2. CETICS Healthcare Technologies GmBH, Esslingen Am Neckar, Esslingen, Deutschland

    Marcel Pilartz & Miriam Unger

  3. CETICS Healthcare Technologies GmbH, Schelztorstraße 54-56, D-73728, Esslingen, Deutschland

    Marcel Pilartz

Authors
  1. Maria Moreno-Villanueva
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  2. Marcel Pilartz
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  3. Miriam Unger
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Correspondence to Marcel Pilartz.

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Moreno-Villanueva, M., Pilartz, M. & Unger, M. Schnelle und aussagekräftige Genotoxizitätsbewertung. Biospektrum 19, 536–538 (2013). https://doi.org/10.1007/s12268-013-0352-7

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  • DOI: https://doi.org/10.1007/s12268-013-0352-7

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