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Genetic diversity and population structure detection in sponge gourd (Luffa cylindrica) using ISSR, SCoT and morphological markers

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Abstract

Investigation of genetic diversity is essential for the selection of parents for crop breeding and conservation of genetic resources. To estimate the genetic variability and population structure in the midst of 45 accessions of sponge gourd brought together from different geographical areas of India, morphological traits and two molecular markers, ISSR and SCoT markers were compared. Principal components analysis of 20 morphological traits showed 72.70% variability and significant positive correlations between fruit traits. All three marker techniques clustered all accessions into two groups with few outgroups. High level of polymorphism was observed among ISSR (74.6%) and SCoT (71.5%) primers. The Bayesian model revealed the hidden grouping and showed admixture type of population. The diversity pattern is influenced by genetic marker used, as different molecular markers have different polymorphism evaluation efficiency. This study can be helpful in amplifying the genetic base and selection of specific traits for breeding. Thus, ISSR and SCoT markers are potential marker for identification in sponge gourd and provide valuable data on its genetic correlation and structure.

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Abbreviations

UPGMA:

Un-weighted pair group method with arithmetic average

ISSR:

Inter simple sequences repeat

PIC:

Polymorphism information content

PCA:

Principal component analysis

SCoT:

Start codon targeted

PCR:

Polymerase chain reaction

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Acknowledgements

We are grateful to the Head, Division of Genomic Resources and the Director, National Bureau of Plant Genetic Resources for providing access to the facilities.

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Author notes

  1. Dipnarayan Saha

    Present address: ICAR-Central Research Institute for Jute and Allied Fibres (ICAR), Barrackpore, India

Authors and Affiliations

  1. Department of Bioscience and Biotechnology, Banasthali University, Banasthali, Rajasthan, 304022, India

    Ruchi Tyagi & Vinay Sharma

  2. Division of Vegetable Science, Indian Agricultural Research Institute, New Delhi, 110012, India

    Amish Kumar Sureja & Anilabh Das Munshi

  3. Division of Genomic Resources, ICAR-NBPGR, New Delhi, 110012, India

    Lalit Arya & Manjusha Verma

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  1. Ruchi Tyagi
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Tyagi, R., Sharma, V., Sureja, A.K. et al. Genetic diversity and population structure detection in sponge gourd (Luffa cylindrica) using ISSR, SCoT and morphological markers. Physiol Mol Biol Plants 26, 119–131 (2020). https://doi.org/10.1007/s12298-019-00723-y

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