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Galactooligosaccharide and a combination of yeast and β-glucan supplements enhance growth and improve intestinal condition in striped catfish Pangasianodon hypophthalmus fed soybean meal diets

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Abstract

The effects of galactooligosaccharide (GOS) and a combination of yeast and β-glucan (YβG) supplementation of dietary soybean meal (SBM) on the growth and digestive performance of striped catfish Pangasianodon hypophthalmus were evaluated. Four isonitrogenous (30% protein) and isocaloric (19 MJ/kg diet) diets were formulated to contain 100% fish meal (FM) protein, 55% FM protein/45% SBM protein, FM-SBM supplemented with 1% GOS, and a combination of 1% yeast and 0.1% β-glucan, respectively. Each diet was fed for 12 weeks to three groups of 30 striped catfish fingerlings (average weight 16.45 ± 0.07 g) maintained in circular fiberglass tanks (600 l). Growth, feed utilization, and muscle protein composition of fish improved significantly after supplementation with either GOS or YβG compared to the unsupplemented SBM diet, but were similar to those of fish fed the FM diet. Nutrient digestibility, digestive enzyme activities, villi and microvilli length were significantly increased in fish fed the supplemented SBM diets. The gut microbiota ranking profile showed that supplementing the SBM diet with YβG and GOS gave a ranking of Verrucomicrobia, Spirochaetes, Bacteriodetes, and Actinobacteria phyla similar to that of the FM diet. Thus, diet containing 45% protein from soybean supplemented with either GOS or YβG can be recommended to improve the growth and digestive performance of striped catfish.

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Acknowledgments

Thanks are due to technical staff at the School of Biological Sciences, Universiti Sains Malaysia. We are grateful to Friesland Foods for supplying Vivinal GOS and to Biorigin, Macrogard for the β-glucan. This study was supported by the Malaysian Ministry of Higher Education ERGS grant no. 203/PBIOLOGY/6730134.

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Authors and Affiliations

  1. Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Aceh, 23111, Indonesia

    Amalia Sutriana

  2. Faculty of Science and Technology, Universiti Sains Islam Malaysia, 71800, Bandar Baru Nilai, Negeri Sembilan, Malaysia

    Roshada Hashim

  3. Faculty of Fisheries, Hajee Mohammad Danesh Science and Technology University, Dinajpur, 5200, Bangladesh

    Mst. Nahid Akter

  4. Institute of Marine Biotechnology, Universiti Malaysia Terengganu, 21300, Kuala Terengganu, Terengganu, Malaysia

    Siti Azizah Mohd Nor

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Correspondence to Amalia Sutriana.

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Sutriana, A., Hashim, R., Akter, M.N. et al. Galactooligosaccharide and a combination of yeast and β-glucan supplements enhance growth and improve intestinal condition in striped catfish Pangasianodon hypophthalmus fed soybean meal diets. Fish Sci 84, 523–533 (2018). https://doi.org/10.1007/s12562-018-1195-4

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