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Comparative Analysis of the Effects of Two Probiotic Bacterial Strains on Metabolism and Innate Immunity in the RAW 264.7 Murine Macrophage Cell Line

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Abstract

Probiotic and potential probiotic bacterial strains are routinely prescribed and used as supplementary therapy for a variety infectious diseases, including enteric disorders among a wide range of individuals. While there are an increasing number of studies defining the possible mechanisms of probiotic activity, a great deal remains unknown regarding the diverse modes of action attributed to these therapeutic agents. More precise information is required to support the appropriate application of probiotics. To address this objective, we selected two probiotics strains, Lactobacillus acidophilus MTCC-10307 (LA) and Bacillus clausii MTCC-8326 (BC) that are frequently prescribed for the treatment of intestinal disorders and investigated their effects on the RAW 264.7 murine macrophage cell line. Our results reveal that LA and BC are potent activators of both metabolic activity and innate immune responses in these cells. We also observed that LA and BC possessed similar activity in preventing infection simulated in vitro in murine macrophages by Salmonella typhimurium serovar enterica.

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Acknowledgments

Authors would like to thank DBT, India, for funding the work from 2010 to 2013. Authors would also acknowledge the facility and infrastructure provided by NISER to execute the work.

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Authors and Affiliations

  1. School of Biological Sciences, National Institute of Science Education and Research (NISER), P.O. Bhimpur-Padanpur, Jatni, Khurdha, Odisha, 752 050, India

    Biswaranjan Pradhan, Dipanjan Guha, Pratikshya Ray, Debashmita Das & Palok Aich

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  1. Biswaranjan Pradhan
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  2. Dipanjan Guha
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  3. Pratikshya Ray
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Correspondence to Palok Aich.

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Pradhan, B., Guha, D., Ray, P. et al. Comparative Analysis of the Effects of Two Probiotic Bacterial Strains on Metabolism and Innate Immunity in the RAW 264.7 Murine Macrophage Cell Line. Probiotics & Antimicro. Prot. 8, 73–84 (2016). https://doi.org/10.1007/s12602-016-9211-4

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