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Adhesion Capacity of Weissella cibaria to Bovine Mammary Tissue and the Effect of Bio-Sealant Topical Application on Physicochemical Properties of Milk

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Abstract

The ability of a probiotic strain (Weissella cibaria) to adhere on tissue and the effect of its topical application in nipples of lactating cows on physicochemical characteristics of milk were evaluated. An ex vivo model was used to demonstrate the adhesion capacity of W. cibaria. Tissue samples were randomly distributed in three different solutions corresponding to three treatments (a nipple bio-sealant formulation, sterile PBS solution and biomass of W. cibaria, sterile PBS solution without microorganism addition). The number of bacteria adhered in tissue was quantified and observed using electron microscopy. Additionally, a bio-sealant prepared with W. cibaria was topically applied to nipples of dairy cows. Milk samples were taken every 7 days for 60 days. Two controls were used. California mastitis test (CMT), somatic cell count, electrical conductivity, pH, density, and acidity were evaluated. The adhesion capacity of W. cibaria strain to epithelial cells of bovine teat tissue samples was demonstrated. When the strain was added as a bio-sealant, the adhesion capacity of W. cibaria was 80.44%. The response variables did not show significant differences among treatments; these results indicate the safety of the topical application of W. cibaria on the bovine mammary gland. In this study, a new safe way of administering probiotic microorganisms in nipples of lactating cows was demonstrated. W. cibaria adheres to the bovine mammary tissue and can be topically applied in nipples of lactating cows without affecting the physicochemical characteristics of milk.

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Acknowledgements

We thank the Universidad Nacional de Colombia for the financing and logistical support of this research.

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Authors and Affiliations

  1. Facultad de Ingeniería y Administración, Universidad Nacional de Colombia Sede Palmira, Palmira, Colombia

    Liliana Serna-Cock

  2. Facultad de Ciencias Agropecuarias, Universidad Nacional de Colombia Sede Palmira, Palmira, Colombia

    Omar Vladimir Pabón-Rodríguez

  3. Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Colombia Sede Manizales, Palmira, Colombia

    Gloria Inés Giraldo-Gómez

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  1. Liliana Serna-Cock
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  2. Omar Vladimir Pabón-Rodríguez
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Correspondence to Liliana Serna-Cock.

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All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

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Serna-Cock, L., Pabón-Rodríguez, O.V. & Giraldo-Gómez, G.I. Adhesion Capacity of Weissella cibaria to Bovine Mammary Tissue and the Effect of Bio-Sealant Topical Application on Physicochemical Properties of Milk. Probiotics & Antimicro. Prot. 11, 1293–1299 (2019). https://doi.org/10.1007/s12602-018-9481-0

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