Skip to main content
SpringerLink
Log in

Isolation and characterization of twelve polymorphic microsatellite loci for investigating an extreme case of microgeographical variation in an island bird (Zosterops borbonicus)

  • Technical Note
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

Twelve polymorphic microsatellite loci were characterized in order to investigate an extreme case of microgeographical variation in an island-dwelling passerine (Zosterops borbonicus). Variation was examined in two distinct natural populations of 27 individuals each. The loci displayed 5–20 alleles, with observed heterozygosities ranging between 0.33 and 0.93. All but one loci (Z16) conformed to Hardy–Weinberg equilibrium. None of the pairwise comparisons among loci showed significant linkage disequilibrium after sequential Bonferonni correction. Successful cross-species amplification in additional Zosterops species and other passerines suggests their broad applicability.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Belkhir K, Borsa P, Chikhi L, Raufaste N, Catch F (2004) GENETIX 4. 0. 5. 2 Software under Windows™ for the genetics of the populations. Laboratory genome, populations, interactions, CNRS UMR 5000. University of Montpellier II, Montpellier

  • Bloor P, De Laguna HB, Kemp SJ (2006) Highly polymorphic tetranucleotide microsatellite loci for the eastern Canary Island lizard, Gallotia atlantica. Mol Ecol Notes 6:737–739. doi:10.1111/j.1471-8286.2006.01326.x

    Article  CAS  Google Scholar 

  • Brownstein MJ, Carpten JD, Smith JR (1996) Modulation of non-templated nucleotide addition by Taq DNA polymerase: primer modifications that facilitate genotyping. BioTechniques 20:1004-6-1008-10

    Google Scholar 

  • Gill FB (1973) Intra-island variation in the Mascarene white-eye Zosterops borbonica. Ornitholog Monogr 12:1–66

    Google Scholar 

  • IUCN (2011) IUCN red list of threatened species. Version 2011.1. http://www.iucnredlist.org. Downloaded on 03 Oct 2011

  • Milá B, Warren BH, Heeb P, Thébaud C (2010) The geographic scale of diversification on islands: genetic and morphological divergence at a very small spatial scale in the Mascarene grey white-eye (Aves: Zosterops borbonicus). BMC Evol Biol 10:158. doi:10.1186/1471-2148-10-158

    Article  PubMed  Google Scholar 

  • Moyle RG, Filardi CE, Smith CE, Diamond J (2009) Explosive pleistocene diversification and hemispheric expansion of a “great speciator”. Proc Natl Acad Sci USA 106:1863–1868. doi:10.1073/pnas.0809861105

    Article  PubMed  CAS  Google Scholar 

  • Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Heredity 86:248–249

    Google Scholar 

  • Rice WM (1989) Analyzing tables of statistical tests. Evol Int J Org Evol 43:223–225. doi:10.2307/2409177

    Article  Google Scholar 

  • Rousset F (2008) Genepop’007: a complete reimplementation of the Genepop software for Windows and Linux. Mol Ecol Resour 8:103–106. doi:10.1111/j.1471-8286.2007.01931.x

    Article  PubMed  Google Scholar 

  • Rozen S, Skaletsky H (2000) Primer 3 on the WWW for general users and for biologist programmers. In: Misener S, Krawetz S (eds) Bioinformatics methods and protocols. Humana Press, Totowa, pp 365–386. doi:10.1385/1-59259-192-2:365

    Google Scholar 

  • van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538. doi:10.1111/j.1471-8286.2004.00684.x

    Article  Google Scholar 

  • Warren BH, Bermingham E, Prys-Jones RP, Thébaud C (2006) Immigration, species radiation and extinction in a highly diverse songbird lineage: white-eyes on Indian Ocean islands. Mol Ecol 15:3769–3786. doi:10.1111/j.1365-294X.2006.03058.x

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

Funding was provided by the IFB, the ANR Biodiversity Program, and the SYNTHESYS Program (http://www.synthesys.info/) of the European Community Research Infrastructure Action under the FP7 “Capacities” Programme at the National Museum of Natural Sciences (CSIC) in Madrid, Spain. We are grateful to Benoit Lequette, Marc Salamolard and Dominique Strasberg for facilitating sample collection, and to Ben Warren for providing blood samples.

Author information

Authors and Affiliations

  1. Laboratoire Evolution et Diversité Biologique, UMR 5174 Centre National de la Recherche Scientifique (CNRS) and Université Paul Sabatier, 118 route de Narbonne, 31062, Toulouse, France

    Joris A. M. Bertrand, Yann Bourgeois, Philipp Heeb & Christophe Thébaud

  2. National Museum of Natural Sciences, Spanish Research Council (CSIC), José Gutiérrez Abascal, 2, 28006, Madrid, Spain

    Ricardo García-Jiménez & Borja Milá

  3. Société Calédonienne d’Ornithologie Nord, BP 236, 98822, Poindimié, Nouvelle-Calédonie, France

    Thomas Duval

Authors
  1. Joris A. M. Bertrand
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ricardo García-Jiménez
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yann Bourgeois
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Thomas Duval
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Philipp Heeb
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Christophe Thébaud
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Borja Milá
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Joris A. M. Bertrand.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bertrand, J.A.M., García-Jiménez, R., Bourgeois, Y. et al. Isolation and characterization of twelve polymorphic microsatellite loci for investigating an extreme case of microgeographical variation in an island bird (Zosterops borbonicus). Conservation Genet Resour 4, 323–326 (2012). https://doi.org/10.1007/s12686-011-9538-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-011-9538-9

Keywords

Search

Navigation