Skip to main content
SpringerLink
Log in

Identification of 246 microsatellites in the Asiatic clam (Corbicula fluminea)

  • Microsatellite Letters
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

Asiatic clam (Corbicula fluminea) is one of the most invasive freshwater bivalves. Despite the ecologic and economic impacts of this species, there are only a few polymorphic microsatellites. In this study, we screened the genome from C. fluminea in search of microsatellite markers using massive parallel sequencing. We identified 246 new microsatellites involving di-, tri-, tetra- and pentanucleotide single repeats and different compound microsatellites, and we used a validation protocol to characterize a sample of those. Nine of them presented two or more alleles indicating a low level of variability.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Benson G (1999) Tandem repeats finder: a program to analyze DNA sequences. Nucleic Acids Res 27:573–580. doi:10.1093/nar/27.2.573

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32:314–331

    PubMed Central  CAS  PubMed  Google Scholar 

  • Peñarrubia L, Sanz N, Pla C, Vidal O, Viñas J (2014) Using Massive Parallel Sequencing for population genetics markers development, validation and application in the invasive bivalve zebra mussel (Dreissena polymorpha)

  • Pigneur LM, Risterucci AM, Dauchot N, Li X, Van DK (2011) Development of novel microsatellite markers to identify the different invasive lineages in the Corbicula complex and to assess androgenesis. Mol Ecol Resour 11:573–577. doi:10.1111/j.1755-0998.2010.02963.x

    Article  CAS  PubMed  Google Scholar 

  • Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234. doi:10.1038/72708

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

We are indebted to Gisela Mir from CRAG Laboratory for her help in obtaining MPS data. The authors thank Cesca Rivas for assistance with laboratory procedures. This research was carried out within the objectives of the Research Project CGL200909407 of the Spanish MICINN.

Author information

Authors and Affiliations

  1. Laboratori d’Ictiologia Genètica, Departament de Biologia, Universitat de Girona, Campus Montilivi, 17071, Girona, Spain

    Luis Peñarrubia, Rosa-Maria Araguas, Carles Pla, Nuria Sanz, Jordi Viñas & Oriol Vidal

Authors
  1. Luis Peñarrubia
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rosa-Maria Araguas
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Carles Pla
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Nuria Sanz
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jordi Viñas
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Oriol Vidal
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Oriol Vidal.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOC 209 kb)

Supplementary material 2 (DOC 199 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Peñarrubia, L., Araguas, RM., Pla, C. et al. Identification of 246 microsatellites in the Asiatic clam (Corbicula fluminea). Conservation Genet Resour 7, 393–395 (2015). https://doi.org/10.1007/s12686-014-0378-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-014-0378-2

Keywords

Search

Navigation