Abstract
Paroaria dominicana, Saltator similis, Sporophila frontalis and Cyanoloxia brissonii are bird species severely exploited by the illegal wildlife trade in Brazil. As part of an effort to characterize the genetic populations within each species to infer the most likely population of origin of seized animals, four microsatellite enriched genomic libraries were constructed and cross amplification of selected primers was tested for all four species. Eight polymorphic loci were selected for P. dominicana with mean observed and expected heterozigosities (mean Ho and He) of 0.750 and 0.768 respectively. Nine were selected for S. similis with mean Ho and He of 0.688 and 0.760. Seven for C. brissonii with mean Ho and He of 0.805 and 0.811. Three loci for S. frontalis with mean Ho and He of 0.851 and 0.903. These markers will be useful for population studies and for the development of paternity tests to be used by commercial breeders.
This is a preview of subscription content, log in via an institution to check access.
References
Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Methods Enzymol 395:202–222
Hamilton MB, Pincus EL, di Fiore A, Fleischer RC (1999) Universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellites. Biotechniques 27(500–2):504–507
Linacre A, Gusmao L, Hecht W, Helmann AP, Mayr WR, Parson W, Prinz M, Schneider PM, Morling N (2011) ISFG: recommendations regarding the use of non-human (animal) DNA in forensic genetic investigations. Forensic Sci Int Genet 5:501–505
Acknowledgments
This work was carried out mostly at the US Fish and Wildlife Forensics Laboratory and supported by the Grant CAPES (#3286-08-8). This work was also supported by FAPESP Grants 07/59747-3 and 08/52207-0. We would like to thank all institutions that contributed with samples: Museu de Zoologia da Universidade de São Paulo, DEPAVE-3, Museu Capão da Imbúia, Laboratório de Genética e Evolução Molecular de Aves (Universidade de São Paulo), Laboratório de Biodiversidade e Evolução Molecular (Universidade Federal de Minas Gerais), Laboratório de Ornitologia e Mastozoologia (Universidade Estadual de Feira de Santana) and SOS Fauna. We are also grateful for all people and institutions which helped us during fieldwork trips. A special thanks for all the staff of the US Fish and Wildlife Forensics Laboratory, specially Ken Goddard and Ed Espinoza.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Ferreira, J.M., Burnham-Curtis, M., Schunck, F. et al. Development of novel polymorphic microsatellite markers for four bird species exploited by the illegal wildlife trade in Brazil. Conservation Genet Resour 7, 435–436 (2015). https://doi.org/10.1007/s12686-014-0389-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-014-0389-z