Abstract
Hummingbird sex identification can be difficult, especially for juveniles. Accurate sex identification is vital for ecology, population health, and conservation biology studies; therefore, a simple molecular technique, that is accurate with less-invasive sampling methods such as feathers, would be useful. Eleven PCR primer pairs reported to identify sex of other bird species were tested in five hummingbird species inhabiting western North America (Anna’s: Calypte anna, Black-chinned: Archilochus alexandri, Allen’s: Selasphorus sasin, Rufous: Selasphorus rufus, and Costa’s Hummingbird: Calypte costae). The two best performing primer pairs were compared using DNA from tissue, blood, and feathers. One primer set was 100 % accurate and optimal for all samples. The less invasive feather sampling method accurately identified sex and has important conservation application for hummingbirds and for ensuring field-sexing data accuracy.
References
Baltosser WH (1987) Age, species, and sex determination of four North American hummingbirds. N Am Bird Bander 12:151–166
Bantock TM, Prys-Jones RP, Lee PLM (2008) New and improved molecular sexing methods for museum bird specimens. Mol Ecol Resour 8:519–528
Bush KL (2005) DNA extraction technique for plucked body contour feathers, molted body contour feathers, and plucked chick down using the QIAamp DNA Micro Kit. Last modified August 22, 2005. http://www.aviangenetics/DNA_extraction_index.htm
Chaves JA, Pollinger JP, Smith TB, LeBuhun G (2007) The role of geography and ecology in shaping the phylogeography of the speckled hummingbird (Adelomyia melanogenys) in Ecuador. Mol Phylogenet Evol 43:795–807
Fridolfsson AK, Ellegren H (1999) A simple and universal method for molecular sexing of non-ratite birds. J Avian Biol 30:116–121
Griffiths R, Double MC, Orr K, Dawson RJG (1998) A DNA test to sex most birds. Mol Ecol 7:1071–1075
Harvey MG, Bonter DN, Stenzler LM, Lovette IJ (2006) print) A comparison of plucked feathers versus blood samples as DNA sources for molecular sexing. J Field Ornithol 77:136–140
Hummingbird Monitoring Network (2012) Web. 16 Nov. 2015. http://www.hummonnet.org
Itoh Y, Ogawa A, Murata K, Hosoda T, Mizuno S (1997) Identification of the sex of oriental white stork, Ciconia boyciana, by the polymerase chain reaction based on its sex chromosome-specific DNA sequences. Genes Genet Syst 72:51–56
Itoh Y, Suzuki M, Ogawa A, Munechika I, Murata K, Mizuno S (2001) Identification of the sex of a wide range of Carinatae birds by PCR using primer sets selected from chicken EE0.6 and its related sequences. J Hered 92:315–321
Jensen T, Pernasetti FM, Durrant B (2003) Conditions for rapid sex determination in 47 avian species by PCR of genomic DNA from blood, shell-membrane blood vessels, and feathers. Zoo Biol 22:561–571
Kahn NW, St John J, Quinn TW (1998) Chromosome-specific intron size differences in the avian CHD gene provide an efficient method for sex identification in birds. Auk 115:1074–1078
Nesje M, Røed KH (2000) Sex identification in falcons using microsatellite DNA markers. Hereditas 132:261–263
Ogawa A, Solovei I, Hutchison N, Saitoh Y, Ikeda J, Macgregor H, Mizuno S (1997) Molecular characterization and cytological mapping of a non-repetitive DNA sequence region from the W chromosome of chicken and its use as a universal probe for sexing Carinatae birds. Chromosome Res 5:93–101
Russell SM, Russell RO (2001) The North American Bander’s manual for banding hummingbirds. North American Banding Council. http://academic.keystone.edu/jskinner/OperationRubythroat/HUMM_MAN.pdf
Sehgal NMR, Jones HI, Smith TB (2005) Molecular evidence for host specificity of parasitic nematode microfilariae in some African rainforest birds. Mol Ecol 14:3977–3988
Stiles FG (1972) Age and sex determination in Rufous and Allen hummingbirds. Condor 74:25–32
Toonen RJ, Hughes S (2001) Increased throughput for fragment analysis on ABI Prism 377 automated sequencer using a membrane comb and STRand software. Biotechniques 31:1320–1324
Acknowledgments
Special thanks to S. Oyler-McCance, US Geological Survey for early guidance in hummingbird DNA analyses and advice. We thank volunteers and personnel from our hummingbird banding teams, the Lindsay Museum Wildlife Hospital, several wildlife rehabilitation centers, the Hummingbird Monitoring Network banding stations, the UC Davis Veterinary Genetics Laboratory, and UC Davis Wildlife Health Center who helped us to collect samples and data, and facilitated our research. Valuable advice and/or sampling assistance was provided by P. Aigner, N. Anderson, S. Bahan, L. Godoy, R. Colwell, L. Dalbeck, B. Hoar, C. Koehler, H. Liu, A. Moresco, N. Pedersen, B. Robinson, L. Rogers, T. Tucker, and S. Wethington. Funding provided by grants from the UC Davis Biology Undergraduate Scholars Program Honors Program (KH), Provost’s Undergraduate Fellowship (KH), US Fish and Wildlife Service (H.B.E.), Academic Senate at UC Davis (H.B.E.), and the Western Hummingbird Partnership (H.B.E.).
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Hagadorn, K.A., Tell, L.A., Drazenovich, T.L. et al. Molecular sex identification markers for five North American hummingbird species. Conservation Genet Resour 8, 427–430 (2016). https://doi.org/10.1007/s12686-016-0587-y
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DOI: https://doi.org/10.1007/s12686-016-0587-y