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Quantitative lateral-flow immunoassay for the assessment of the cartilage oligomeric matrix protein as a marker of osteoarthritis

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Abstract

This study reports a simple and quantitative analytical method for detecting cartilage oligomeric matrix protein (COMP) using a lateral-flow immunoassay (LFI) format. An immuno-chromatographic assay was developed using a gold nanoparticle (AuNP)-conjugated monoclonal antibody (Ab) probe for the quantitative detection of COMP in human synovial fluid (SF). The detection antibody was conjugated with the AuNP (∼30 nm) to enable detection, and the capture antibody was immobilized within a multi-spot pattern in the test zone of a membrane strip. COMP in the human SF binds to the AuNP-conjugated detection antibody, and the COMP-Ab complex then binds to the capture antibody. The immunoassay test can be analyzed by visualizing the test strip with a commercial scanner and an imaging software program. The color density of the multi-spot pattern at the test zone increased in proportion to the concentration of COMP. The developed immunoassay exhibited a dynamic detection range from 0.6 to 20 μg/mL of COMP. The detection limit of the proposed LFI system was approximately 0.2 μg/mL. The test results showed good correlation with those obtained using a conventional enzyme-linked immunosorbent assay (ELISA) to detect COMP. We expect that the proposed immunoassay method, based on the quantification of COMP, is suitable for osteoarthritis diagnosis using SF samples.

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Authors and Affiliations

  1. Department of Molecular Science & Technology, Ajou University, Suwon, 443-749, Korea

    Sung Yub Hong, Yoo Min Park, Yo Han Jang, Byoung-Hyun Min & Hyun C. Yoon

  2. Department of Orthopedic Surgery, School of Medicine, Ajou University, Suwon, 443-749, Korea

    Byoung-Hyun Min

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  1. Sung Yub Hong
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  2. Yoo Min Park
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  3. Yo Han Jang
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  4. Byoung-Hyun Min
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  5. Hyun C. Yoon
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Correspondence to Hyun C. Yoon.

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Hong, S.Y., Park, Y.M., Jang, Y.H. et al. Quantitative lateral-flow immunoassay for the assessment of the cartilage oligomeric matrix protein as a marker of osteoarthritis. BioChip J 6, 213–220 (2012). https://doi.org/10.1007/s13206-012-6303-4

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  • DOI: https://doi.org/10.1007/s13206-012-6303-4

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