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Differential protein expression associated with heat stress in Antarctic microalga

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Abstract

We used proteomic approaches to study the survival and defense mechanisms of Antarctic microalga under heat stress. The microalga was cultured for optimal growth at 4°C, the temperature was increased to 15°C, and the resultant differentially expressed proteins induced by heat stress were analyzed by mass spectrometry following two-dimensional gel electrophoresis. Orthologues of 22 proteins showed more than two-fold changes in abundance; of these, 8 proteins were up-regulated, and 14 were down-regulated. In addition, changes in the enzyme activities and isozyme profiles of catalase, aldehyde dehydrogenase, superoxide dismutase, glutathione reductase, and ascorbate peroxidase were investigated using an in-gel activitystaining method. Alterations in protein expression and antioxidant enzyme activity in Antarctic algae may be related to survival and defense mechanisms against elevated temperatures.

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  1. Department of Medical Biotechnology, SoonChunHyang University, Asan, Chungnam, 336-600, Korea

    Kwang Man Choi & Mi Young Lee

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  1. Kwang Man Choi
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  2. Mi Young Lee
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Correspondence to Mi Young Lee.

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Choi, K.M., Lee, M.Y. Differential protein expression associated with heat stress in Antarctic microalga. BioChip J 6, 271–279 (2012). https://doi.org/10.1007/s13206-012-6310-5

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  • DOI: https://doi.org/10.1007/s13206-012-6310-5

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