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SsPsaH, a H subunit of the photosystem I reaction center of Suaeda salsa, confers the capacity of osmotic adjustment in tobacco

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Abstract

Background

Abiotic stress effects agricultural production, so research on improving stress tolerance of crop is important. Suaeda salsa is a halophyte with high salt and drought tolerance and ability to desalinate saline soil and improve soil quality.

Objective

To discover and utilize of salt and drought tolerance-related genes, we further investigated the mechanisms of salt and drought tolerance.

Methods

Through screening a salt treated Suaeda salsa cDNA library and further cloning a H subunit of the photosystem I reaction center SsPsaH cDNA, and then the protein domain and phylogenetic analyses of PSI genes was conducted with the NCBI Blast, DNAMAN, and MotifScan programs. The S. salsa seedlings were subjected to various stress treatments and analyze expression of SsPsaH under these treatments by real-time RT-PCR. SsPsaH expression construct was introduced into S. pombe cells by electroporation and transformed into N. tabacum plants by the leaf disc transformation method.

Results

A member of the H subunit of the Photosystem I reaction center (defined as SsPsaH) was obtained. The expression of SsPsaH was up-regulated by abscisic acid (ABA), salt, and drought stress treatments. Over-expressing SsPsaH in recombinant yeasts enhanced high salinity tolerance and increased tolerance to sorbitol during seed germination and seedling root development in tobacco, respectively. Some stress-related mark genes such as a LEA family gene of NtLEA, a binding protein of a drought response element of NtDREB, the ascorbate peroxidase gene (NtAPX) were also up-regulated in SsPsaH overexpressing transgenic tobacco lines.

Conclusions

These results show that SsPsaH may contribute to the salt and osmotic stress response of plants.

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Funding

This work was supported by Natural Science Foundation of Xinjiang, China (No. 2015211A028) and Tianshan Youth Projects of Xinjiang, China (No. 2017Q051).

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Authors and Affiliations

  1. Institute of Nuclear and Biological Technology, Xinjiang Academy of Agricultural Sciences, Urumqi, 830091, China

    Xiaoyan Hao, Jianping Li, Shengqi Gao, Zumuremu Tuerxun, Xiaochun Chang, Wenran Hu, Guo Chen & Quansheng Huang

  2. Xinjiang Key Laboratory of Crop Biotechnology, Xinjiang Academy of Agricultural Sciences, Urumqi, 830091, China

    Xiaoyan Hao, Jianping Li, Shengqi Gao, Zumuremu Tuerxun, Xiaochun Chang, Wenran Hu, Guo Chen & Quansheng Huang

Authors
  1. Xiaoyan Hao
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  2. Jianping Li
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  3. Shengqi Gao
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  4. Zumuremu Tuerxun
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  5. Xiaochun Chang
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  6. Wenran Hu
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  7. Guo Chen
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  8. Quansheng Huang
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Contributions

XH and QH conceived and designed the experiments; JL, SG, ZT, XC, WH and CG conducted the biochemical and some genetic assays; XH and QH analyzed the data, wrote the manuscript. All authors have read and approved the final manuscript.

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Correspondence to Quansheng Huang.

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Hao, X., Li, J., Gao, S. et al. SsPsaH, a H subunit of the photosystem I reaction center of Suaeda salsa, confers the capacity of osmotic adjustment in tobacco. Genes Genom 42, 1455–1465 (2020). https://doi.org/10.1007/s13258-020-00970-4

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  • DOI: https://doi.org/10.1007/s13258-020-00970-4

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