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Clustered and dispersed chromosomal distribution of the two classes of Revolver transposon family in rye (Secale cereale)

  • Plant Genetics • Original Paper
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Abstract

The chromosomal locations of a new class of Revolver transposon-like elements were analyzed by using FISH method on the metaphase chromosome in somatic cell division of the rye cultivar Petkus. First, the Revolver standard element probe λ2 was weakly hybridized throughout the rye chromosome, and comparatively large interstitial signals spotted with a dot shape were detected together with several telomeric regions. The dot shape interstitial signal was stably detected at one site on Chromosome (Chr) 1R (middle part of the interstitial region of the short arm), three sites on Chr 2R (distal part of the interstitial region and adjacent to the centromere on the short arm, middle part of the interstitial region of the long arm), and two sites on Chr 5R (middle part of the interstitial region and adjacent to the centromere on the long arm). The Revolver λ2 probe was effective for identification of 1R, 2R, and 5R chromosomes. On the other hand, Revolver nonautonomous element-specific L626-BARE-100 probe was strongly distributed throughout the rye chromosomes, and considerable numbers and diverse lengths of transcripts were detected by RT-PCR. Although the standard elements were found in localized clusters, the nonautonomous elements tended to be dispersed throughout the genome. Clustered nature of Revolver is a significantly rare case in genomics.

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Funding

This work was funded by the Grant-in-Aid for Scientific Research No. 1360006 and No. 04760006 of the Japanese Ministry of Education, Culture, Sports, Science and Technology (MEXT) to Motonori Tomita.

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Authors and Affiliations

  1. Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan

    Motonori Tomita

  2. Faculty of Agriculture, Tottori University, 4-101 Koyama Minami, Tottori, 680-8550, Japan

    Takaaki Kanzaki & Eri Tanaka

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  1. Motonori Tomita
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  2. Takaaki Kanzaki
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  3. Eri Tanaka
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MT conceived and designed the study; MT, TK, and ET conducted the experiments and analyzed the data; MT wrote the manuscript; all authors read and approved submission.

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Correspondence to Motonori Tomita.

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Communicated by: Izabela Pawłowicz

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Tomita, M., Kanzaki, T. & Tanaka, E. Clustered and dispersed chromosomal distribution of the two classes of Revolver transposon family in rye (Secale cereale). J Appl Genetics 62, 365–372 (2021). https://doi.org/10.1007/s13353-021-00617-4

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