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Forensic DNA analyses suggest illegal trade of canid skins

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Abstract

Accurate identification of species and populations is essential for biodiversity conservation and for monitoring of international trade in wild species. Morphological and behavioural traits are not always reliable for species identification of samples of unknown origin, and suspected hybridization augments this challenge. We used mitochondrial DNA (mtDNA) markers to identify 24 canid skins illegally imported from Mongolia to Denmark in 1997. Our results indicated that the skins included both wolf (Canis lupus) and dog (Canis lupus familiaris) haplotypes. One of the wolf haplotypes was new. A phylogenetic analysis revealed that the skins shared haplotypes with wolves in Europe and Mongolia, which appears to support the assumption of a Mongolic origin though a different provenance cannot be excluded. We observed an apparent discrepancy between the skins’ morphological traits and the mtDNA identification for 10 out of 24 individuals. This might suggest the presence of hybrids, but poor quality DNA precluded reliable microsatellite typing to investigate hybridization. At present, the scarcity of published records for wolf mtDNA sequences from central Asia limits more detailed investigation and we recommend increased efforts to document genetic variation in this region.

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References

  • Andersen LW, Harms V, Caniglia R, Czarnomska SD, Fabbri E, Jędrzejewska B, Kluth G, Nowak C, Pertoldi C, Randi E, Reinhardt I, Stronen AV (2015) Long-distance dispersal of a wolf, Canis lupus, in northwestern Europe. Mamm Res 60:163–168

    Article  Google Scholar 

  • Ardalan A, Oskarsson MC, Asch B, Rabakonandriania E, Savolainen P (2015) African origin for Madagascan dogs revealed by mtDNA analysis. R Soc Open Sci 2:140552

    Article  PubMed  PubMed Central  Google Scholar 

  • Caniglia R, Fabbri E, Mastrogiuseppe L, Randi E (2013) Who is who? Identification of livestock predators using forensic genetic approaches. Forensic Sci Int Genet 7:397–404

    Article  CAS  PubMed  Google Scholar 

  • Chetri M, Jhala YV, Jnawali SR, Subedi N, Dhakal M, Yumnam B (2016) Ancient himalayan wolf (Canis lupus chanco) lineage in upper mustang of the annaourna conservation area, Nepal. ZooKeys 582:143–156

  • Djan M, Maletić V, Trbojević I, Popović D, Veličković N, Burazerović J, Ćirović D (2014) Genetic diversity and structuring of the grey wolf population from the Central Balkans based on mitochondrial DNA variation. Mamm Biol 79:277–282

    Google Scholar 

  • Elias M, Hill RI, Willmott KR, Dasmahapatra KK, Brower AVZ, Mallet J, Jiggins CD (2007) Limited performance of DNA barcoding in the diverse community of tropical butterflies. Proc R Soc B 274:2881–2889

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Galov A, Fabbri E, Caniglia R, Arbanasić H, Lapalombella S, Florijančić T, Bošković I, Galaverni M, Randi E (2015) First evidence of hybridization between golden jackal (Canis aureus) and domestic dog (Canis familiaris) as revealed by genetic markers. R Soc Open Sc 2:150450

    Article  Google Scholar 

  • Godinho R, Llaneza L, Blanco JC, Lopes S, Álvares F, García EJ, Palacios V, Cortés Y, Talegón J, Ferrand N (2011) Genetic evidence for multiple events of hybridization between wolves and domestic dogs in the Iberian Peninsula. Mol Ecol 20:5154–5166

    Article  PubMed  Google Scholar 

  • Hindrikson M, Männil P, Ozolins J, Krzywinski A, Saarma U (2012) Bucking the trend in wolf-dog hybridization: first evidence from Europe of hybridization between female dogs and male wolves. PLoS One 7(10):e46465

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Jansson E, Harmoinen J, Ruokonen M, Aspi J (2014) Living on the edge: reconstructing the genetic history of the Finnish wolf population. BMC Evol Biol 14:64

    Article  PubMed  PubMed Central  Google Scholar 

  • Kraus RHS, von Holdt B, Cocchiararo B, Harms V, Bayerl H, Kühn R, Förster DW, Fickel J, Roos C, Nowak C (2015) A single-nucleotide polymorphism-based approach for rapid and cost-effective genetic wolf monitoring in Europe based on noninvasively collected samples. Mol. Ecol Res 14:295–305

    Article  Google Scholar 

  • Mech D, Boitani L (2003) Wolves: behavior, ecology, and conservation. The University of Chicago Press, Chicago

    Book  Google Scholar 

  • Monzon J, Kays R, Dykhuizen DE (2014) Assessment of coyote-wolf-dog admixture using ancestry-informative diagnostic SNPs. Mol Ecol 23:182–197

    Article  CAS  PubMed  Google Scholar 

  • Pilot M, Branicki W, Jędrzejewski W, Goszczyński J, Jędrzejewska B, Dykyy I, Shkvyrya M, Rsingarska E (2010) Phylogeographic history of grey wolves in Europe. BMC Evol Biol 10:104

    Article  PubMed  PubMed Central  Google Scholar 

  • Randi E, Lucchini V (2002) Detecting rare introgression of domestic dog genes into wild wolf (Canis lupus) populations by Bayesian admixture analyses of microsatellite variation. Conserv Genet 3:31–45

    Article  CAS  Google Scholar 

  • Randi E, Hulva P, Fabbri E, Galaverni M, Galov A, Kusak J, Bigi D, Bolfíková BČ, Smetanová M, Caniglia R (2014) Multilocus detection of wolf x dog hybridization in Italy, and guidelines for marker selection. PLoS One 9(1):e86409

    Article  PubMed  PubMed Central  Google Scholar 

  • Randi E, Lucchini V, Christensen MF, Mucci N, Funk SM, Dolf G, Loeschcke V (2000) Mitochondrial DNA variability in Italian and East European wolves: detecting the consequences of small population size and hybridization. Conserv Biol 14:464–473

    Article  Google Scholar 

  • Ronquist R, Teslenko M, Van der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Lui L, Suchard M, Huelsenbeck JP (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst Biol 61:539–542

    Article  PubMed  PubMed Central  Google Scholar 

  • Savolainen P, Zhang Y, Luo J, Lundeberg J, Leitner T (2002) Asian origin of domestic dogs. Science 298:1610–1613

    Article  CAS  PubMed  Google Scholar 

  • Snyder-Mackler N, Majoros WH, Yuan ML, Shaver AO, Gordon JB, Kopp GH, Schlebusch SA, Wall JD,Alberts SC, Mukherjee S, Zhou X, Tung J (2016) Efficient genome-wide sequencing and low coverage pedigree analysis from non-invasively collected samples. Genetics. doi:10.1534/genetics.116.187492

  • Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Thalmann O, Shapiro B, Cui P, et al. (2013) Complete mitochondrial genomes of ancient canids suggest a European origin of domestic dogs. Science 342:871–874

    Article  CAS  PubMed  Google Scholar 

  • Vilà C, Walker C, Sundqvist AK, Flagstad Ø, Andersone Z, Casulli A, Kojola I, Valdmann H, Halverson J, Ellegren H (2003) Combined use of maternal, paternal and bi-parental genetic markers for the identification of wolf-dog hybrids. Heredity 90:17–24

    Article  PubMed  Google Scholar 

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Acknowledgments

We thank Aalborg Zoo and Naturstyrelsen for loan of skins and information. We thank Aarhus University for laboratory space, Linda Hartun Konggaard for laboratory assistance, and reviewers for valuable comments that improved the manuscript.

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Authors and Affiliations

  1. Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, 9220, Aalborg, Denmark

    Camilla Lerbjerg Thomsen & Astrid Vik Stronen

  2. Department of Bioscience, Aarhus University, Kalø, Grenåvej 14, 8410, Rønde, Denmark

    Liselotte Wesley Andersen

Authors
  1. Camilla Lerbjerg Thomsen
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  2. Liselotte Wesley Andersen
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  3. Astrid Vik Stronen
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Correspondence to Astrid Vik Stronen.

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This work was supported by Aalborg Zoo Conservation Foundation (AZCF).

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Communicated by: Cino Pertoldi

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Thomsen, C.L., Andersen, L.W. & Stronen, A.V. Forensic DNA analyses suggest illegal trade of canid skins. Mamm Res 61, 423–426 (2016). https://doi.org/10.1007/s13364-016-0296-7

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  • DOI: https://doi.org/10.1007/s13364-016-0296-7

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