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Genome-wide identification and expression analysis of sulphate transporter (SULTR) genes under sulfur deficiency in Brachypodium distachyon

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Abstract

Sulphur is an important mineral element for plant growth and development. It involves in a number of metabolic processes with crucial functions. This study has performed a genome-wide analysis of sulfate transporter (SULTR) genes in Brachypodium distachyon. Ten putative SULTR genes were identified in Brachypodium genome. BdSULTR genes included 6–17 exons encoding a protein of 647–693 residues with basic nature. BdSULTR proteins included both sulfate_transp (PF00916) and STAS (PF01740) domains. BdSULTRs were classified into 4 groups based on the phylogenetic distribution. Promoter regions of all BdSULTR genes, except for BdSULTR3;3 and 3;5 included the SURECOREATSULTR11 elements. A considerable structural overlap was identified between superimposed SULTR1;3 and 3;1 proteins, indicating that SULTR1 members may also involve in plant stress response/tolerance like SULTR3 members. Microarray and RNA-Seq analyses also revealed the differential expression of SULTR 1 and 3 genes under different biotic/abiotic stresses. Protein–protein interaction partners of BdSULTRs were mainly related with adenylyl-sulfate kinases, 5′-adenylylsulfate reductases, ATP sulfurylases, and acyl carrier proteins. Moreover, expression profiles of identified BdSULTR genes under S-deficiency were analyzed using RT-qPCR. It was revealed that BdSULTR1;1 and 3;1 are highly expressed in plant roots as ~tenfold and ~fivefold, respectively, while BdSULTR2 (~15-fold) and 3;1 (~twofold) are abundantly expressed in leaf tissues.

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Abbreviations

SULTR:

Sulfate transporters

CELLO:

subCELlularLOcalization predictor

GEO:

Gene Expression Omnibus

CRE:

cis-regulatory elements

TMDs:

Transmembrane domains

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Authors and Affiliations

  1. Department of Biology, Faculty of Science and Arts, Fatih University, Buyukçekmece, Istanbul, Turkey

    Huseyin Tombuloglu & Mehtap Aydın

  2. Department of Crop and Animal Production, Cilimli Vocational School, Duzce University, 81750, Cilimli, Duzce, Turkey

    Ertugrul Filiz

  3. Department of Molecular Biology and Genetics, Faculty of Science, Gebze Technical University, 41400, Gebze, Kocaeli, Turkey

    Ibrahim Koc

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  1. Huseyin Tombuloglu
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Tombuloglu, H., Filiz, E., Aydın, M. et al. Genome-wide identification and expression analysis of sulphate transporter (SULTR) genes under sulfur deficiency in Brachypodium distachyon . J. Plant Biochem. Biotechnol. 26, 263–273 (2017). https://doi.org/10.1007/s13562-016-0388-0

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