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Calcium signalling mediated the regulation of growth and polysaccharide accumulation by light quality in Dendrobium officinale protocorms

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Abstract

This study selected Dendrobium officinale protocorms as the experimental material and used different light-quality treatments and calcium signalling inhibitors to explore the mechanism of the biological response of calcium to light quality. The results indicate that a 1:3 red:blue (R:B) light ratio promotes the accumulation of biomass and polysaccharides in the protocorm. Nevertheless, this effect can be entirely counteracted by calmodulin (CaM) inhibitors and calcium ion-chelating agents. The possible mechanisms of this effect are as follows: (1) The Ca2+-CaM signal-regulated photosynthetic apparatus is improved by the R:B 1:3 light, increasing the CaM content and Ca2+-ATPase activity. Thus, R:B 1:3 light increases the development of the photosynthetic apparatus in the D. officinale protocorm, enhances its photosynthetic productivity, and promotes the activities of sucrose phosphatase; accordingly, biomass and sucrose accumulation increase. (2) R:B 1:3 light enhances Ca2+-CaM signalling and regulates glycometabolic processes to promote the activities of sucrose synthase, thus increasing fructose and glucose levels and providing sufficient synthetic precursors to promote polysaccharide production.

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Data availability

The datasets generated during and analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

This work was supported by the National Key R&D Program of China (NO.2018YFD0600105), Sichuan Science and Technology Program (NO. 2018JY0211), and Sichuan Landscape and Recreation Research Center (JGYQ2019024).

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Authors and Affiliations

  1. Institute of Garden, Sichuan Agricultural University, Huimin Road No. 211, Wenjiang District, Chengdu, 611130, Sichuan Province, People’s Republic of China

    Ting Lei, Suping Gao, Yingqi Liu, Wenji Li & Di Hu

  2. College of Landscape Architecture, Sichuan Agricultural University, Chengdu, 611130, Sichuan Province, People’s Republic of China

    Ting Lei, Suping Gao, Yingqi Liu, Xiaofang Yu, Mingyan Jiang & Qibing Chen

  3. Greenland Headquarter, Huangpu, 200023, Shanghai, People’s Republic of China

    Xiao Lin

  4. Department of plant science , Chong Qing Municipal Agricultural School, Chongqing, 401329, Chongqing, People’s Republic of China

    Kaihui Zhang

  5. Sichuan Province Landscape Flower Engineering Laboratory, Sichuan Agricultural University, Chengdu, 611130, Sichuan Province, People’s Republic of China

    Qibing Chen

  6. Chinese Academy of Sciences Sichuan Translational Medicine Research Hospital, Chengdu, 610031, Sichuan Province, People’s Republic of China

    Ying Sun

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  1. Ting Lei
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Contributions

T.L, experimental design, instrument operation, statistical analysis. S.G*, provide overall thinking. X.L, experimental design, instrument operation. K.Z, instrument operation. Y.L, statistical analysis. W.L, measure, material preparation. D.H, material preparation. X.Y, experimental design. M.J, material preparation. Q.C, provide overall thinking. Y.S, measure.

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Correspondence to Suping Gao.

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The authors declare that they do not have any commercial or associative interest that represents a conflict of interest in connection with the work submitted.

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Communicated by So-Young Park, Ph.D.

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Lei, T., Gao, S., Lin, X. et al. Calcium signalling mediated the regulation of growth and polysaccharide accumulation by light quality in Dendrobium officinale protocorms. Hortic. Environ. Biotechnol. 62, 287–297 (2021). https://doi.org/10.1007/s13580-020-00304-z

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