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Pesticide-induced oxidative stress in laboratory and field populations of native honey bees along intensive agricultural landscapes in two Eastern Indian states

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Abstract

Pesticides have been cited as one of the major drivers of pollinator loss. However, little is known about pesticide impacts on natural populations of native honey bee species. This study looked into the effect of pesticides with respect to oxidative stress in the laboratory and in field populations of two native Indian honey bee species (Apis dorsata and A. cerana) by examining a combination of biomarkers, e.g., superoxide dismutase, catalase and xanthine oxidase. A significant upregulation of all three biomarkers was observed in both treated individuals in laboratory experiments and field populations sampled from a pesticide use gradient. This study reports, for the first time, an increase in expression of xanthine oxidase in an invertebrate system (honey bees) exposed to pesticides.

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ACKNOWLEDGMENTS

The study was supported by an INSPIRE fellowship from the Department of Science & Technology, Govt. of India to Priyadarshini Chakrabarti; the Department for Environment, Food and Rural Affairs (DEFRA) Darwin Initiative grant (1662) to Parthiba Basu and Barbara Smith, and a research grant to Sagartirtha Sarkar from the Department of Science & Technology, Govt. of India. Soumik Chatterjee, Debaditya Kumar, Ritam Bhattacharya and Arnob Chatterjee helped in obtaining bee samples. Dr. Kaberi Samanta helped with the GIS maps. The Department of Agriculture, Governments of Odisha and West Bengal helped by providing the cropping intensity data. SGS India Private Limited analysed pesticide residues in soil and honey bee bodies.

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Authors and Affiliations

  1. Department of Zoology, University of Calcutta, 35, B.C. Road, Kolkata, 700019, India

    Priyadarshini Chakrabarti, Santanu Rana, Sagartirtha Sarkar & Parthiba Basu

  2. Centre for Pollination Studies, University of Calcutta, 35, B.C. Road, Kolkata, 700019, India

    Priyadarshini Chakrabarti, Barbara Smith & Parthiba Basu

  3. Game & Wildlife Conservation Trust, Burgate Manor, Fordingbridge, Hampshire, SP6 1EF, UK

    Barbara Smith

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  1. Priyadarshini Chakrabarti
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  2. Santanu Rana
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  4. Barbara Smith
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  5. Parthiba Basu
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Correspondence to Parthiba Basu.

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Manuscript editor: Monique Gauthier

Stress oxydatif provoqué par des pesticides sur des populations indigènes d’abeilles, élevées en laboratoire et en champ, dans des zones d’agriculture intensive de deux Etats de l’est de l’Inde

Apis cerana / Apis dorsata / enzyme / dismutase / catalase / oxydase / insecticide

Durch Pestizide verursachter oxydativer Stress bei Labor. und Feldpopulationen einheimischer Honigbeienen entlang zweier intensiver Agrarlandschaften in zwei ostindischen Staaten

Apis cerana / Apis dorsata / Enzyme / Superoxid-Dismutase / Xanthin-Oxidase / Katalase / Insektizid

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APPENDIX

APPENDIX

List of abbreviations used in methodology section:

Abbreviation

Full terminology

Abbreviation

Full terminology

HIC

High intensity cropping

XOX

Xanthine oxidase

LIC

Low intensity cropping

PBS

Phosphate buffered saline

LCMS/MS

Liquid chromatography–mass spectrometry / mass spectrometry

NaCl

Sodium chloride

GC

Gas chromatography

NP40

Tergitol-type NP-40 (nonylphenoxypolyethoxylethanol)

EPA3540C and EPA8081A procedures

Environmental Protection Agency methods 3540C and 8081A; followed by SGS India Pvt. Ltd.

EDTA

Ethylenediaminetetraacetic acid

OP

Organophosphorus pesticide

SDS-PAGE

Sodium dodecyl sulfate—Polyacrylamide gel electrophoresis

EC

Effective Concentration

PVDF

Polyvinylidenedifluoride

SP

Synthetic pyrethroid

HRP

Horseradish peroxidase

ES

Endosulfan pesticide

DTPA

Diethylene triamine pentaacetic acid

Experimental treatments

D0 (control)

D1 (5 % OP + 1.5 % SP + 5.5 % ES)

D2 (12.5 % OP + 4 % SP + 15 % ES)

D3 (20 % OP + 6.5 % SP + 23.5 % ES)

D4 (25 % OP + 8 % SP + 29 % ES)

D5 (30 % OP + 10 % SP + 35 % ES)

Tris–HCl

Tris (2-Amino-2-hydroxymethyl-propane-1,3-diol)—Hydrochloric acid

SOD

Superoxide dismutase

CAT

Catalase

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Chakrabarti, P., Rana, S., Sarkar, S. et al. Pesticide-induced oxidative stress in laboratory and field populations of native honey bees along intensive agricultural landscapes in two Eastern Indian states. Apidologie 46, 107–129 (2015). https://doi.org/10.1007/s13592-014-0308-z

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