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Neuroprotective effect of chia seed oil nanoemulsion against rotenone induced motor impairment and oxidative stress in mice model of Parkinson’s disease

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Abstract

Chia seed oil (CSO) was reported to possess various pharmacological effects, however, its usefulness is restricted due to its inadequate solubility, bioavailability and stability. In the present work, efforts were put forward to develop chia seed oil nanoemulsion (CSO NE). The developed CSO NE was exposed to pharmacodynamic evaluation against Parkinson’s disease (PD) induced by rotenone (RT) in the mice. Here, the animals were classified into 6 groups: (I) Vehicle control, (II) RT (1 mg/kg s.c.), (III) CSO (200 mg/kg p.o.) + RT, (IV) CSO (400 mg/kg p.o.) + RT, (V) CSO NE (200 mg/kg p.o.) + RT, (VI) CSO NE (400 mg/kg p.o.) + RT. Animals received the treatment 30 min before RT administration for 14 days. The outcomes of the motor/behavioural evaluations (rotarod test and locomotor activity), biochemical evaluations (estimation of malondialdehyde, nitrite, acetylcholine esterase, reduced glutathione, catalase and superoxide dismutase) and histopathological evaluation affirmed that the CSO NE treatment rendered a significant enhancement in the neuroprotective effects as compared to CSO administered alone. These results suggest that the oral bioavailability of CSO was escalated by its conversion to nanoform, thus imparting greater neuroprotection. The potential application of CSO NE was established in the management of PD.

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Acknowledgements

The authors express gratitude to the College of Pharmaceutical Sciences, Dayananda Sagar University, Bengaluru, Karnataka for their support and facilities provided to fulfil this project. There is no funding received for this article.

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  1. Department of Pharmacology, College of Pharmaceutical Sciences, Dayananda Sagar University, Kumaraswamy Layout, Bangalore, Karnataka, 560078, India

    K. M. Geetha & Jyotsna Shankar

  2. Department of Pharmaceutics, College of Pharmaceutical Sciences, Dayananda Sagar University, Kumaraswamy Layout, Bangalore, Karnataka, 560078, India

    Barnabas Wilson

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Correspondence to K. M. Geetha.

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All applicable guidelines for the care and use of animals were followed. The experimental protocols were approved by the institutional animal ethics committee (DSU/M.Pharm/IACE/17/2020–21) and conducted according to the CPCSEA guidelines, New Delhi, India.

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Jyotsna Shankar has no conflict of interest. Geetha K.M. has no conflict of interest. Barnabas Wilson has no conflict of interest.

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Geetha, K.M., Shankar, J. & Wilson, B. Neuroprotective effect of chia seed oil nanoemulsion against rotenone induced motor impairment and oxidative stress in mice model of Parkinson’s disease. ADV TRADIT MED (ADTM) 23, 1091–1108 (2023). https://doi.org/10.1007/s13596-022-00648-0

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