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Positive enhancement of Lactobacillus fermentum HY01 on intestinal movements of mice having constipation

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Abstract

Lactobacilli have been used to treat many gastrointestinal disorders. But the outcome of Lactobacilli are strain specific. The strain Lactobacillus Fermentum, HY01, (LF-HY01) has a good performance in the environment of gastrointestinal tract. In this study, the aim is to investigate the preventive effects of LF-HY01 against activated-carbon-induced constipation in mice. Mice are randomized into four groups. Normal group was fed a normal diet, model group also has the same with activated carbon treatment, and low and high concentration groups are treated with LF-HY01. We have determined many indexes such as body weight, water content in faeces, defecation conditions, the level of small intestinal villi damages and levels of various neurotransmitters in serum, including motilin (MTL), gastrin (GT), endothelin (ET), somatostatin (SST), acetylcholinesterase (AchE), substance P (SP), and vasoactive intestinal peptide (VIP). LF-HY01 has no significant difference in each group, but it can significantly improve water content of faeces, defecation time of first black stool and activated carbon propelling rate in small intestine as compared of model group. Furthermore, LF-HY01 can effectively prevent small intestinal villi damages, which is less than that of model group. Moreover, LF-HY01 has the consistency to increase the levels of MTL, GT, ET, AchE, SP and VIP, and LF-HY01 can also have the ability to reduce the level of SST. These results suggest that Lactobacillus Fermentum, HY01, has a great impact in enhancing intestinal peristalsis ability and has the ability to prevent from activated-carbon-induced constipation in mice.

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Acknowledgment

This work was partly supported by Special Fund for Agro-scientific Research in the Public Interest (201303085), the People’s Livelihood Science and technology innovation Foundation of Chongqing (cstc2015shmszx80021), Fundamental Research Funds for the Central Universities (XDJK2016A018), Research Project of Sichuan Key Laboratory of Bamboo Diseases and Insect Pests Prevention and Control, and Resource Development (17ZZ016), the Construction Program of Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education (167001) and Chongqing Research Program of Basic Research and Frontier Technology (cstc2016jcyjA0339), China.

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  1. Xiaoyong Chen and Jia-Le Song have contributed equally to this work.

Authors and Affiliations

  1. College of Food Science, Southwest University, 2 Tiansheng Road, Beibei District, Chongqing, 400715, People’s Republic of China

    Xiaoyong Chen, Hongwei Wang & Huayi Suo

  2. Chongqing Engineering Research Center of Regional Food, Chongqing, 400715, People’s Republic of China

    Xiaoyong Chen, Hongwei Wang & Huayi Suo

  3. Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing, 400067, People’s Republic of China

    Xiaoyong Chen & Xin Zhao

  4. Department of Nutrition and Food Hygiene, School of Public Health, Guilin Medical University, Guilin, 541004, Guangxi, People’s Republic of China

    Jia-Le Song

  5. College of Life Science, Leshan Normal University, Leshan, 614000, People’s Republic of China

    Qiang Hu

  6. Bamboo Diseases and Pests Control and Resources Development Key Laboratory of Sichuan Province, Leshan, 614000, People’s Republic of China

    Qiang Hu

  7. School of Biological and Chemical Engineering, Chongqing University of Education, 9 Xuefu Main Street, Nan’an District, Chongqing, 400067, People’s Republic of China

    Xin Zhao

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  1. Xiaoyong Chen
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  2. Jia-Le Song
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  3. Qiang Hu
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  4. Hongwei Wang
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  5. Xin Zhao
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  6. Huayi Suo
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Correspondence to Xin Zhao or Huayi Suo.

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Chen, X., Song, JL., Hu, Q. et al. Positive enhancement of Lactobacillus fermentum HY01 on intestinal movements of mice having constipation. Appl Biol Chem 61, 39–48 (2018). https://doi.org/10.1007/s13765-017-0327-3

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