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The Biocompatibility of Multi-Source Stem Cells and Gelatin-Carboxymethyl Chitosan-Sodium Alginate Hybrid Biomaterials

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Background:

Nowadays, biological tissue engineering is a growing field of research. Biocompatibility is a key indicator for measuring tissue engineering biomaterials, which is of great significance for the replacement and repair of damaged tissues.

Methods:

In this study, using gelatin, carboxymethyl chitosan, and sodium alginate, a tissue engineering material scaffold that can carry cells was successfully prepared. The material was characterized by Fourier transforms infrared spectroscopy. In addition, the prepared scaffolds have physicochemical properties, such as swelling ratio, biodegradability. we observed the biocompatibility of the hydrogel to different adult stem cells (BMSCs and ADSCs) in vivo and in vitro. Adult stem cells were planted on gelatin-carboxymethyl chitosan-sodium alginate (Gel/SA/CMCS) hydrogels for 7 days in vitro, and the survival of stem cells in vitro was observed by live/died staining. Gel/SA/CMCS hydrogels loaded with stem cells were subcutaneously transplanted into nude mice for 14 days of in vivo culture observation. The survival of adult stem cells was observed by staining for stem cell surface markers (CD29, CD90) and Ki67.

Results:

The scaffolds had a microporous structure with an appropriate pore size (about 80 μm). Live/died staining showed that adult stem cells could stably survive in Gel/SA/CMCS hydrogels for at least 7 days. After 14 days of culture in nude mice, Ki67 staining showed that the stem cells supported by Gel/SA/CMCS hydrogel still had high proliferation activity.

Conclusion:

Gel/SA/CMCSs hydrogel has a stable interpenetrating porous structure, suitable swelling performance and degradation rate, can promote and support the survival of adult stem cells in vivo and in vitro, and has good biocompatibility. Therefore, Gel/SA/CMCS hydrogel is a strong candidate for biological tissue engineering materials.

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Acknowledgements

This work was supported by Shandong Provincial Natural Science Foundation of China (No. ZR2020MH070 and No. ZR2020MH078), Shandong Province Medicine and Health Science and Technology Development Plan Project (No. 2019WS368), and Research Support Foundation of Jining Medical University (No. JYFC2018FKJ009 and No. JYFC2018KJ004). Xinzhe Wang: Methodology, Investigation, Formal analysis, Writing-original draft. Siqi Li: Investigation, Data curation. Honglian Yu: Conceptualization, Formal analysis, Writing-review & editing, Supervision, Project administration. Jianzhi Lv: Validation, Data curation. Minglun Fan: Investigation. Ximing Wang: Validation. Xin Wang: Validation. Yanting Liang: Validation. Lingna Mao: Validation. Zhankui Zhao: Supervision, Writing—review & editing.

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Authors and Affiliations

  1. Clinical Medical College, Jining Medical University, Jining, 272067, Shandong, China

    Xinzhe Wang, Jianzhi Lv, Minglun Fan, Ximing Wang & Xin Wang

  2. Medical Research Center, Affiliated Hospital of Jining Medical University, Jining, 272067, Shandong, China

    Siqi Li

  3. Department of Biochemistry, Jining Medical University, Jining, Shandong, 272067, People’s Republic of China

    Honglian Yu

  4. Collaborative Innovation Center, Jining Medical University, Jining, Shandong, 272067, People’s Republic of China

    Honglian Yu

  5. Basic Medicine College, Jining Medical University, Jining, Shandong, 272067, People’s Republic of China

    Yanting Liang & Lingna Mao

  6. Department of Urology, Affiliated Hospital of Jining Medical University, Jining Medical University, Jining, Shandong, 272100, People’s Republic of China

    Zhankui Zhao

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  1. Xinzhe Wang
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Correspondence to Zhankui Zhao.

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All animal experiments were conducted in the Central Laboratory of Affiliated Hospital of Jining Medical University, according to the guidelines of the Institutional Animal Care and Use Committee and the Declaration of Helsinki.

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Wang, X., Li, S., Yu, H. et al. The Biocompatibility of Multi-Source Stem Cells and Gelatin-Carboxymethyl Chitosan-Sodium Alginate Hybrid Biomaterials. Tissue Eng Regen Med 19, 491–503 (2022). https://doi.org/10.1007/s13770-021-00429-x

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  • DOI: https://doi.org/10.1007/s13770-021-00429-x

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