Skip to main content
SpringerLink
Log in

Morpho-Molecular Identification of Lasiodiplodia theobromae Causing Root Rot of Mulberry in India

  • Short Communication
  • Published:
National Academy Science Letters Aims and scope Submit manuscript

Abstract

The root rot disease caused by Lasiodiplodia theobromae in Morus alba emerged as a serious problem that affect sustainable mulberry production. In this study, the disease symptoms like yellowing of leaves, blackening, and rotting of roots were observed. The associated pathogen was isolated from infected root tissue and pure culture was raised. Based on morphological features, ITS sequencing, and phylogenetic analyses, the fungal pathogen is identified as Lasiodiplodia theobromae. The sequence was deposited in NCBI with the accession number MK811183. The pathogenicity was confirmed by artificial inoculation on mulberry seedlings. This disease can cause great economic loss, as every part of the plant is infected. So, correct identification of disease to take timely management measures is a must, this basic work will give proper identification of pathogen and help to improvise the mulberry production in the country.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

References

  1. Singhal BK, Khan MA, Dhar A, Baqual FM, Bindroo BB (2010) Approaches to industrial exploitation of mulberry (mulberry sp.) fruits. J Fruit Ornam Plant Res 18(18):83–99

    Google Scholar 

  2. Tikader A, Kamble CK (2008) Mulberry wild species in India and their use in crop improvement a review. Aust J Crop Sci 2(2):64–72

    Google Scholar 

  3. Vijayan K, Kar PK, Tikader A, Srivastava PP, Awasthi AK, Thangavelu K, Saratchandra B (2004) Molecular evaluation of genetic variability in wild populations of mulberry (Morus serrata Roxb). Plant Breed 123(6):568–572

    Article  CAS  Google Scholar 

  4. Sengupta AR, Gupta R, Biswas A (2019) Computational fluid dynamics analysis of stove systems for cooking and drying of muga silk. Emerg Sci J 3(5):285–292

    Article  Google Scholar 

  5. Zou S, Chen W (2003) A review on chemical constituents, pharmological activity and application of mulberry leaves. J. Chem. Ind. For. Products 1:1–8

    Google Scholar 

  6. Zhang M, Chen M, Zhang HQ, Sun S, Xia B, Wu FH (2009) In vivo hypoglycemic effects of phenolics from the root bark of Morus alba. Fitoterapia 80(8):475–477

    Article  CAS  Google Scholar 

  7. Jan B, Parveen R, Zahiruddin S, Khan MU, Mohapatra S, Ahmad S (2021) Nutritional constituents of mulberry and their potential applications in food and pharmaceuticals: a review. Saudi J Biol Sci 28(7):3909–3921

    Article  CAS  Google Scholar 

  8. Sharma DD, Naik VN, Chowdary NB, Mala VR (2003) Soilborne diseases of mulberry and their management. Int J Ind Ent 7(2):93–106

    Google Scholar 

  9. Gupta VR, Govindaiah RHV (1997) Diseases and associated pathogens of mulberry nurseries. Indian Phyto-pathol 50:402–407

    Google Scholar 

  10. Zhang YJ, Zhang S, Liu XZ, Wen HA, Wang M (2010) A simple method of genomic DNA extraction suitable for analysis of bulk fungal strains. Lett Appl Microbial 51(1):114–118

    Google Scholar 

  11. White TJ, Bruns TS, Lee SB, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic press, New York, pp 315–322

    Google Scholar 

  12. Kumar S, Stecher G, Tamura K (2016) Molecular evolutionary genetics analysis version 70 for bigger datasets. Mol Biol Evol 33(7):1870–1874

    Article  CAS  Google Scholar 

  13. Xie HH, Wei JG, Liu F, Pan XH, Yang XB (2014) First report of mulberry root rot caused by Lasiodiplodia theobromae in China. Plant Dis 98(11):1581–1581

    Article  Google Scholar 

  14. Punithalingam E (1976) Botryodiplodia theobromae. CMI Descriptions of Pathogenic Fungi and Bacteria, No. 519. Commonwealth Mycological Institute, Key, Surrey, England

  15. Latha P, Prakasam V, Kamalakannan A, Gopalakrishnan C, Raguchander T, Paramathma M, Samiyappan R (2009) First report of Lasiodiplodia theobromae (Pat) Griffon & Maubl causing root rot and collar rot disease of physic nut (Jatropha curcas L.) in India. Australas Plant Dis Notes 4(1):19–20

    Google Scholar 

  16. Nam MH, Park MS, Kim HS, Kim TI, Lee EM, Park JD, Kim HG (2016) First report of dieback caused by Lasiodiplodia theobromae in strawberry plants in Korea. Mycobiology 44(4):319–324

    Article  Google Scholar 

  17. Kumar R, Kumari A, Sinha A, Maurya S (2014) New report of Lasiodiplodia theobromae causing Jatropha decline in Eastern Plateau and Hill region of India. Arch Phytopathol Plant Prot 47(11):1286–1290

    Article  Google Scholar 

  18. Pereira OL, Dutra DC, Dias LAS (2009) Lasiodiplodia theobromae is the causal agent of a damaging root and collar rot disease on the biofuel plant Jatropha curcas in Brazil. Australas Plant Dis Notes 4(1):120–123

    Google Scholar 

  19. Haggag WM (2014) First report of Lasiodiplodia theobromae causing canker and collar rot diseases of physic nut (Jatropa curcas ) in Egypt. J Plant Pathol 96(3):603–611

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Plant Pathology, GKVK, University of Agricultural Sciences, Bengaluru, 560065, India

    R. Kanyakumari, Y. M. Somasekhara & S. Basavaraj

  2. Division of Entomology, ICAR- Indian Agricultural Research Institute, New Delhi, 110012, India

    M. G. Deeksha

Authors
  1. R. Kanyakumari
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Y. M. Somasekhara
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Basavaraj
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. G. Deeksha
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Significance Statement This work is carried out to find the causal organism of mulberry root rot disease. Here, symptoms caused by the pathogen on host plant, its morphological character and molecular identification reveals that Lasiodiplodia theobromae is the causal organism. Further, pathogenicity test also validates the above prediction.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kanyakumari, R., Somasekhara, Y.M., Basavaraj, S. et al. Morpho-Molecular Identification of Lasiodiplodia theobromae Causing Root Rot of Mulberry in India. Natl. Acad. Sci. Lett. 45, 451–456 (2022). https://doi.org/10.1007/s40009-022-01161-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40009-022-01161-3

Keywords

Search

Navigation