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Physiological and biochemical markers to optimize sugar mill wastewater for irrigation in maize (Zea mays L.) crop

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Abstract

The current study represents the effect of varying concentrations (0, 25, 50, 75, and 100 %) of sugar mill wastewater on growth, biochemistry, and physiology of two maize cultivars (Pak-Afgoi and EV-5098) at seedling, silking, and grain-filling growth stages. It was observed that lower levels of sugar mill wastewater (25 and 50 %) resulted in increase in chlorophyll contents and plant fresh and dry masses. In addition, sugar mill wastewater caused a consistent increase in RMP, SOD activity, MDA and H2O2 concentrations, and decrease in ascorbic acid concentration and activities of POD and CAT. Of the two cultivars, hazardous effects of high concentrations of sugar mill wastewater were more prominent in cv. EV-5098 that exhibited lower chlorophyll contents, fresh and dry masses and lower activities of enzymatic antioxidants and concentrations of non-enzymatic antioxidants. However, cv. Pak-Afgoi tolerated higher levels of sugar mill wastewater in terms of lower endogenous concentrations of MDA and H2O2 and better antioxidant defense system. Of different growth stages, seedling and grain-filling stages proved to be more sensitive to the application of sugar mill wastewater. Overall, the results suggested that diluted sugar mill wastewater (≤50 %) can be used safely for irrigation purpose of maize plants.

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Abbreviations

SOD:

Superoxide dismutase

POD:

Peroxidase

CAT:

Catalase

MDA:

Malondialdehyde

H2O2 :

Hydrogen peroxide

RMP:

Relative membrane permeability

References

  • Abbas ST, Sarfraz M, Mehdi SM, Hassan G, Rehman OU (2007) Trace elements accumulation in soil and rice plants irrigated with the contaminated water. Soil Tillage Res 94:503–509

    Article  Google Scholar 

  • Aina OA, Ilelabayo MO, Oju RI, Azubuike VC, Isaac AA, Ebenezer OF (2012) Long-term exposure to industrial effluent induces oxidative stress and affects growth in Clarias gariepinus. Res J Environ Earth Sci 4:738–746

    Google Scholar 

  • Ajmal M, Khan A (1985) Effect of textile industry effluent on soil and crop plant. Environ Pollut A137:131–148

    Article  Google Scholar 

  • Arnon DI (1949) Copper enzyme in isolated chloroplasts Polyphenoloxidase in Beta vulgaris. J Plant Physiol 24:1–15

    Article  CAS  Google Scholar 

  • Ashraf M (2009) Biotechnological approach of improving plant salt tolerance using antioxidants as markers. Biotechnol Adv 27:84–93

    Article  CAS  PubMed  Google Scholar 

  • Ashraf MA, Ashraf M, Shabaz M (2012) Growth stage based modulation in antioxidant defense system and proline accumulation in two haploid wheat (Triticum aestivum L.) cultivars differing in salinity tolerance. Flora 207:388–397

    Article  Google Scholar 

  • Ayyasamy PM, Yasodha R, Rajakumar S, Lakshmanaperumalsamy P, Rahman PKSM, Lee S (2008) Impact of sugar factory effluent on the growth and biochemical characteristics of terrestrial and aquatic plants. Bull Environ Contam Toxicol 81:449–454

    Article  CAS  PubMed  Google Scholar 

  • Baskran L, Sundaramoorthy P, Chidambaram ALA, Ganesh KS (2009) Growth and physiological activity of green gram (Vigna radiata L.) under effluent stress. Bot Res Int 2:107–114

    Google Scholar 

  • Bates IS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–207

    Article  CAS  Google Scholar 

  • Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287

    Article  CAS  PubMed  Google Scholar 

  • Bharagava RN, Chandra R, Rai V (2010) Phytoextraction of trace elements and physiological changes in Indian mustard plants (Brassica nigra L.) grown in postmethunated distillery effluent (PMDE) irrigated soil. Bioresour Technol 99:8316–8324

    Article  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  PubMed  Google Scholar 

  • Cakmak I, Strbac D, Marschner H (1993) Activities of hydrogen peroxide scavenging enzymes in germinating wheat seeds. J Exp Bot 44:127–132

    Article  CAS  Google Scholar 

  • Chandra R, Bharagava RN, Yadav S, Mohan D (2009) Accumulation and distribution of toxic metals in wheat (Triticum aestivum) and Indian mustard irrigated with distillery and tannery effluent. J Hazard Mater 162:1514–1521

    Article  CAS  PubMed  Google Scholar 

  • Davis BH (1976) Carotenoids. In: Goodwin TW (ed) chemistry and biochemistry of plant pigments. Academic press, London, San Francisco, pp 138–165

    Google Scholar 

  • Ezhilvannan D, Sharavanan PS, Vijayaragavan M (2011) Effect of sugar mills effluent on changes of growth and amino acids and protein contents of maize (Zea mays L.) plants. J Ecobiotechnol 3:26–29

  • Gallego SM, Benavides MP, Tomaro ML (1996) Effect of heavy metal ion excess on sunflower leaves: evidence for involvement of oxidative stress. Plant Sci 121:151–159

    Article  CAS  Google Scholar 

  • Gomathi V, Oblisami G (1992) Effect of pulp and paper mill effluent on germination of tree species. Ind J Environ Health 34:326–328

  • Gupta AK, Sinha S (2007) Phytoextraction capacity of the plants growing on tannery sludge dumping sites. Bioresour Technol 9:1788–1794

    Article  Google Scholar 

  • Hamilton PB, Van-Slyke DD (1943) Amino acid determination with ninhydrin. J Biol Chem 150:231–233

    CAS  Google Scholar 

  • Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts. Arch Environ Contam Toxicol 125:189–198

    CAS  Google Scholar 

  • Hussain I, Iqbal M, Nawaz M, Rasheed R, Perveen A, Mahmood S, Yasmeen A, Wahid A (2013) Effect of sugar mill effluent on growth and antioxidative potential of maize seedling. Int J Agric Biol 15:1227–1235

    CAS  Google Scholar 

  • Iqbal S, Younas U, Chan KM, Saeed Z, Shaheen MA (2013) Growth and antioxidant response of Brassica rapa var. rapa L. (turnip) irrigated with different compositions of paper and board mill (PBM) effluent. Chemosphere 91:1196–1202

    Article  CAS  PubMed  Google Scholar 

  • Kannan K, Upreti RK (2008) Influence of distillery effluent on germination and growth of mung bean (Vigna radiata) seeds. J Hazard Mater 153:609–615

    Article  CAS  PubMed  Google Scholar 

  • Kaushik A, Nisha R, Jagjeeta K, Kaushik CP (2005) Impact of long and short term irrigation of a sodic soil with distillery effluent in combination with bio amendments. Bioresour Technol 96:1860–1866

    Article  CAS  PubMed  Google Scholar 

  • Lasa B, Frechilla S, Aleu M, Gonzalez-Moro B, Lamsfus C, Aparicio-Tejo PM (2000) Effects of low and high levels of magnesium on the response of sunflower plants grown with ammonium and nitrate. Plant Soil 225:167–174

  • Misra RN, Behera PK (1991) The effects of paper mill industry effluent on growth, pigment, carbohydrates and protein content of rice seedling. Environ Pollut 72:159–167

    Article  CAS  PubMed  Google Scholar 

  • Mukherjee SP, Choudhuri MA (1983) Implication of water stress induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings. Physiol Plant 58:166–170

  • Oncel I, Keles Y, Ustun AS (2000) Interactive effects of temperature and heavy metal stress on the growth and some biochemical compounds in wheat seedlings. Environ Pollut 107:315–320

    Article  CAS  PubMed  Google Scholar 

  • Pulver EL, Ries SK (1973) Action of simazine in increasing plant protein contents. Weed Sci Soc Am 21:233–237

    CAS  Google Scholar 

  • Rathore NP, Iqbal SA, Pawan KS (2000) Role of sugar Industry effluent in agriculture. Ind J Appl Pure Biol 19:91–94

    Google Scholar 

  • Rowe DR, Abdel-Magid IM (1995) Hand book of waste water reclamation and reuse. CRC Press Inc, Boca Raton, p 550

    Google Scholar 

  • Sairam RK, Rao KV, Srivastava GC (2002) Differential response of wheat genotypes to long-term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Sci 163:1037–1046

    Article  CAS  Google Scholar 

  • Salequzzaman M, Islam SMT, Tasnuva A, Kashem MA, Masud MMA (2008) Environmental impact of sugar industry a case study on Kushtia sugar mills in Bangladesh. J Innov Dev Strat 2:31–35

    Google Scholar 

  • Singh S, Sinha S (2005) Accumulation of metals and its effects in Brassica juncea L. (cv. Rohini) grown on various amendments of tannery waste. Ecotoxicol Environ Saf 62:118–127

    Article  CAS  PubMed  Google Scholar 

  • Snedecor GW, Cochran WG (1980) Statistical methods, 7th edn. The Iowa State University Press, Ames, Iowa

  • Thambavani D, Sabitha MA (2011) Impact of sugar mill effluent on physico-chemical profile of affected soil. Environ Pollut 5:49–54

    CAS  Google Scholar 

  • Velikova V, Yordanova I, Edreva A (2000) Oxidative stress and some antioxidant systems in acid rain-treated bean plants: protective role of exogenous polyamines. Plant Sci 151:59–66

    Article  CAS  Google Scholar 

  • WHO (2004) Guidelines for drinking water quality. Recommendations, vol 1, 3rd edn WHO, Geneva. http://www.who.int/water_sanitation_health/wastewater/en/. Accessed 27 Nov 2014

  • Yang X, Baligar VC, Martens DC, Clark PB (1996) Plant tolerance to nickel toxicity: II nickel effect on influx and transport of mineral nutrients in four plant species. J Plant Nutr 19:265–279

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Authors and Affiliations

  1. Department of Botany, Government College University, Faisalabad, Pakistan

    Rizwan Rasheed, Muhammad Arslan Ashraf, Iqbal Hussain, Abida Parveen, Rizwan Alam & Muhammad Iqbal

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  1. Rizwan Rasheed
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  2. Muhammad Arslan Ashraf
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  3. Iqbal Hussain
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  4. Abida Parveen
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  5. Rizwan Alam
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  6. Muhammad Iqbal
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Correspondence to Rizwan Rasheed.

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Rasheed, R., Ashraf, M.A., Hussain, I. et al. Physiological and biochemical markers to optimize sugar mill wastewater for irrigation in maize (Zea mays L.) crop. Braz. J. Bot 38, 51–61 (2015). https://doi.org/10.1007/s40415-014-0122-x

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  • DOI: https://doi.org/10.1007/s40415-014-0122-x

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