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Effect of Silymarin Supplementation on Nickel Oxide Nanoparticle Toxicity to Rainbow Trout (Oncorhynchus mykiss) Fingerlings: Pancreas Tissue Histopathology and Alkaline Protease Activity

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Abstract

The effects of dietary nickel oxide nanoparticles (NiO-NPs) contamination along with silymarin supplementation on pancreatic tissue of rainbow trout fingerlings were investigated. Five treatments including different levels of NiO-NPs (0, 100 and 500 mg kg−1 feed) and silymarin (0 and 1 g kg−1 feed) in three respective replicates were designed. The trial was conducted under 12L:12D photoperiod condition for 60 days. Tissue samples for histological and enzymatic studies were taken on days 30 and 60. The results from day 30 indicated that the highest and lowest alkaline protease activities were belonged to fish fed diet without any nanoparticles (T1) and those fed 100 or 500 mg kg−1 feed (T4 and T5), respectively. Dietary silymarin could to some extent prevent toxic effects of NiO-NPs on enzyme activity (T2 vs. T3). Acinar cells necrosis, edema of connective tissue and cellular shrinkage were observed in NiO-NPs received groups (T4 and T5). At the end of the trial, T2 and T3 could regain their digestive capacity after removal of nanoparticles and those groups continued to receive nanoparticles until the end of the trial (T4 and T5) showed the lowest alkaline protease activity. Moreover, the histological observations revealed pancreas tissue necrosis, nuclear degeneration and vascular dilation in the latter groups. In conclusion, NiO-NPs affected pancreatic tissue and its function in a dose-dependent manner, while dietary silymarin helped fish to sustain digestive capacity. Future researches should focus on the ecological outcomes of pollutants induced toxicity.

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Acknowledgments

The authors would like to express their sincere gratitude to Vice-Chancellor for Research and Technology of Urmia University, I.R. Iran, for financial support of the research and also to Mr Jalili for his assistance in diet preparation and sampling. We are also thankful to staffs of Laboratory of Aquaculture, Urmia Lake Research Institute for their close collaborations during fish husbandry. We also appreciate the constructive suggestions and criticism given by two anonymous reviewers which certainly elaborated the quality of the manuscript.

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  1. Department of Fisheries, Faculty of Natural Resources, Urmia University, Nazlou Campus, 11th km of Serow Road, Urmia, Islamic Republic of Iran

    Nina Nazdar, Ahmad Imani & Kourosh Sarvi Moghanlou

  2. Urmia Lake Research Institute, Urmia University, Urmia, Islamic Republic of Iran

    Farzaneh Noori

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  1. Nina Nazdar
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  2. Ahmad Imani
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Correspondence to Ahmad Imani.

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Nazdar, N., Imani, A., Noori, F. et al. Effect of Silymarin Supplementation on Nickel Oxide Nanoparticle Toxicity to Rainbow Trout (Oncorhynchus mykiss) Fingerlings: Pancreas Tissue Histopathology and Alkaline Protease Activity. Iran J Sci Technol Trans Sci 42, 353–361 (2018). https://doi.org/10.1007/s40995-016-0052-5

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