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Androgen deprivation causes up-regulation of androgen receptor transcript in the rat prostate

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Abstract

We have studied the effect of androgenic deprivation on the level of androgen receptor transcript in the rat ventral prostate. The rats were treated with estradiol benzoate, flutamide and [D Trp6, des Gly10]gonadotropin releasing hormone (GnRH) for different time periods. These treatments produced a significant decrease in the weight of prostate. Total RNA isolated from the ventral prostates was hybridized with the cDNA probe for androgen receptor. Densitometric analysis of the autoradiographic signal revealed a rise in the level of androgen receptor RNA following treatment of rats with estradiol benzoate and flutamide. Treatment of rats with [D Trp6, des Gly10] GnRH brought about a transient rise in the level of androgen receptor RNA. Thus, our results indicate that androgenic deprivation up-regulates the level of androgen receptor transcript.

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Authors and Affiliations

  1. Department of Pharmacology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, 110 029

    Vijay L. Kumar & Pradip K. Majumder

  2. Virology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110 067, India

    Vijay Kumar

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  1. Vijay L. Kumar
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  2. Pradip K. Majumder
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Kumar, V.L., Majumder, P.K. & Kumar, V. Androgen deprivation causes up-regulation of androgen receptor transcript in the rat prostate. Mol Cell Biochem 171, 133–138 (1997). https://doi.org/10.1023/A:1006805503284

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