Abstract
The involvement of the carbohydrate components of the Azospirillum brasilenseSp245 capsules in bacterial protection from the action of extreme factors was investigated. The survival of encapsulated and non-encapsulated azospirilla exposed to elevated (46–48°C) and below-freezing (–20 and –70°C) temperatures, extreme pH values (2 and 10), and to drying was studied. High-molecular-weight carbohydrate-containing complexes (lipopolysaccharide–protein complex and polysaccharide–lipid complex) were isolated from the capsular material of azospirilla. It was shown that the addition of these complexes to the suspension of decapsulated cells before exposing them to extreme factors enhanced their survival rates by 15 to 51%.
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Berg, R.H., Tyler, M.E., Novick, N.J., Vasil, V., and Vasil, I.K., Biology of Azospirillum–Sugarcane Association: Enhancement of Nitrogenase Activity, Appl. Environ. Microbiol., 1980, vol. 39, pp. 642–649.
Jofre, E., Rivarola, V., Balegno, H., and Mori, G., Differential Gene Expression in Azospirillum brasilense Cd Under Saline Stress, Can. J. Microbiol., 1998, vol. 44, no. 10, pp. 929–936.
Lamm, R.B. and Neyra, C.A., Characterization and Cyst Production of Azospirilla Isolated from Selected Grasses Growing in New Jersey and New York, Can. J. Microbiol., 1981, vol. 27, pp. 1320–1325.
Neyra, C.A., Atkinson, A., and Olubayi, O., Coaggregation of Azospirillum with Other Bacteria: Basis for Functional Diversity, Azospirillum VI and Related Microorganisms, Fendrik, I. et al., Eds., Berlin: Springer, 1995, vol. 37, pp. 429–439.
Fages, J., An Optimized Process for Manufacturing an Azospirillum Inoculant for Crops, Appl. Microbiol. Biotechnol., 1990, vol. 32, pp. 473–478.
Sadasivan, L. and Neyra, C.A., Flocculation in Azospirillum brasilense and Azospirillum lipoferum: Exopolysaccharides and Cyst Formation, J. Bacteriol., 1985, vol. 163, pp. 716–723.
Skvortsov, I.M. and Ignatov, V.V., Extracellular Polysaccharides and Polysaccharide-containing Biopolymers from Azospirillum Species: Properties and the Possible Role in Interaction with Plant Roots, FEMS Microbiol. Lett., 1998, vol. 165, pp. 223–229.
Konnova, S.A., Makarov, O.E., Skvortsov, I.M., and Ignatov, V.V., Isolation, Fractionation, and Some Properties of Polysaccharides Produced in a Bound Form by Azospirillum brasilense and Their Possible Involvement in Azospirillum– Wheat Root Interactions, FEMS Microbiol. Lett., 1994, vol. 118, no. 2, pp. 93–99.
Egorenkova, I.V., Konnova, S.A., Fedonenko, Yu.P., Dykman, L.A., and Ignatov, V.V., The Role of the Polysaccharide-containing Capsular Components of Azospirillum brasilense in Bacterial Adsorption on Wheat Seedling Roots, Mikrobiologiya, 2001, vol. 70, no. 1, pp. 45–50.
Vande Broek and Vanderleyden, J., Genetics of the Azospirillum–Plant Root Association, Crit. Rev. Plant Sci., 1995, vol. 14, no. 5, pp. 445–466.
Pirog, T.P., Grinberg, T.A., and Malashenko, Yu.R., Protective Function of the Polysaccharide Synthesized by Acinetobacter sp., Mikrobiologiya, 1997, vol. 66, no. 3, pp. 335–340.
Dufrne, Y.F. and Rouxhet, P.G., Surface Composition, Surface Properties, and Adhesiveness of Azospirillum brasilense: Variation during Growth, Can. J. Microbiol., 1996, vol. 42, pp. 548–556.
Matora, L., Solovova, G., Serebrennikova, O., Selivanov, N., and Shchyogolev, S., Immunological Properties of Azospirillum Cell Surface: The Structure of Carbohydrate Antigens and Evaluation of Their Involvement in Bacteria–Plant Contact Interactions, Azospirillum VI and Related Microorganisms, Fendrik, I. et al., Eds., Berlin: Springer, 1995, vol. 37, pp. 377–382.
Konnova, S.A., Makarov, O.E., Skvortsov, I.M., and Ignatov, V.V., The Exopolysaccharides of Azospirillum brasilense Sp245 and Sp107, Mikrobiol. Zh. (Kiev), 1992, vol. 54, no. 2, pp. 31–42.
Rokitskii, P.F., Biologicheskaya statistika (Biostatistics), Minsk: Vysheishaya Shkola, 1973, p. 320.
Yagoda-Shagam, J., Barton, L.L., Reed, W.P., and Chiovetti, R., Fluorescein Isothiocyanate–labeled Lectin Analysis of the Surface of the Nitrogen-fixing Bacterium Azospirillum brasilense by Flow Cytometry, Appl. Environ. Microbiol., 1988, vol. 54, no. 7, pp. 1831–1837.
Munblit, V.Ya., Tal'roze, V.L., and Trofimov, V.I., Termoinaktivatsiya mikroorganizmov (Thermoinactivation of Microorganisms), Moscow: Nauka, 1985, p. 248.
Stephan, M.P., Pedrosa, F.O., and Dbereiner, J., Physiological Studies with Azospirillum spp. Associative N2-Fixation, Florida: Boca Raton, 1981, pp. 8–12.
Kushner, D.J., Life of Microorganisms at High Salt and Solute Concentrations, Microbial Life in Extreme Environments, Kushner, D.J., Ed., London: Academic, 1978. Translated under the title Zhizn' mikrobov v ekstremal'nykh usloviyakh, Moscow: Mir, 1981.
Aktual'nye problemy kriobiologii (Topical Problems in Cryobiology), Pushkar', N.S. and Belous, A.M., Eds., Kiev: Naukova Dumka, 1981.
Vorob'ev, V.A., Simbioticheskaya azotfiksatsiya i temperatura (The Effect of Temperature on Symbiotic Nitrogen Fixation), Novosibirsk: Nauka, 1998.
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Konnova, S.A., Brykova, O.S., Sachkova, O.A. et al. Protective Role of the Polysaccharide-containing Capsular Components of Azospirillum brasilense. Microbiology 70, 436–440 (2001). https://doi.org/10.1023/A:1010434227671
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DOI: https://doi.org/10.1023/A:1010434227671