Skip to main content
SpringerLink
Log in

Random amplified polymorphic DNA (RAPD) analysis in Indian mung bean (Vigna radiata (L.) Wilczek) cultivars

  • Published:
Genetica Aims and scope Submit manuscript

Abstract

Greengram [Vigna radiata (L.) Wilczek], also known as mung bean, widely cultivated in a large number of countries, is an important pulse crop of Asia and is considered one of the ancestral species of the genus Vigna. Since yields of greengram have remained low across subtropical and tropical Asia, it is important to estimate genetic diversity in existing cultivars in order to see if the lack of genetic variability might be a constraining factor. In this study, 32 Indian cultivars of greengram were subjected to random amplified polymorphic DNA (RAPD) analysis using 21 decamer primers. A total of 267 amplification products were formed at an average of 12.71 per primer with an overall polymorphism of 64%. The extent of polymorphism was moderate to low. Jaccard similarity coefficient values ranged from 0.65 to 0.92. The cluster analysis resulted in mainly three clusters revealing greater homology between cultivars released from the same source. The results of principal components analysis also substantiated this conclusion. The close genetic similarity between the cultivars could be explained due to the high degree of commonness in their pedigrees. The narrow genetic base of the greengram cultivars revealed in the present analysis emphasises the need to exploit the large germplasm collections having diverse morphoagronomic traits in cultivar improvement programs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ahmad, F., 1999. Random Amplified Polymorphic DNA analysis reveals genetic relationships among annual Cicer species. Theor. Appl. Genet. 98: 657–663.

    Article  CAS  Google Scholar 

  • Asemota, H.N., J. Ramser, C. Lopez-Perolta, K. Weising & G. Kohl, 1996. Genetic variation and cultivar identification of Jamaican yam germplasm by random amplified polymorphic DNA analysis. Euphytica 92: 341–351.

    Article  CAS  Google Scholar 

  • Asthana, A.N. & S.K. Chaturvedi, 1999. A little impetus needed, pp. 61–66 in The Hindu-Survey of Indian Agriculture 1999, edited by N. Ravi. Chennai, India.

    Google Scholar 

  • Bhat, K.V., S. Chadha & S. Lakhanpaul, 1999. Study of genetic diversity in Indian and exotic seasme (Sesamum indicum L.) germplasm using random amplified polymorphic DNA (RAPD) markers. Euphytica 110: 21–33.

    Article  CAS  Google Scholar 

  • Bhat, K.V. & R.L Jarret, 1995. Random amplified polymorphic DNA and genetic diversity in Indian Musa germplasm. Genet. Res. Crop Evol. 42: 107–118.

    Google Scholar 

  • Bisht, I.S., R.K. Mahajan & T.G Kawalkar, 1998. Diversity in greengram (Vigna radiata (L.) Wilczek) germplasm collection and its potential use in crop improvement. Ann. Appl. Biol. 132: 301–312.

    Article  Google Scholar 

  • Bisht, I.S., R.K. Mahajan & D.P Patel, 1998. The use of characterization data establish the Indian mung bean core collection and assessment of genetic diversity. Genet. Res. Crop Evol. 45: 127–133.

    Article  Google Scholar 

  • Brunk, C.F., K.C. Jones & T.W. James, 1979. Assay for nanogram quantities of DNA in cellular homogenates. Anal. Biochem. 92: 497–500.

    Article  PubMed  CAS  Google Scholar 

  • Cao, W., P. Huel, G. Scoles & R.N. Chibbar, 1998. Genetic diversity between spelta and macha wheats based on RAPD analysis. Euphytica 104: 181–189.

    Article  Google Scholar 

  • de Condolle A., 1886. Origin of Cultivated Plants. Hafner Publishing, New York.

    Google Scholar 

  • Dana, S., 1966. The cross between Phaseolus aureus Roxb. and P. mungo L. Genetica 37: 259–274.

    Article  Google Scholar 

  • Davila, J.A., Y. Loarce & E. Ferrer, 1999. Molecular characterization and genetic mapping of random amplified microsatellite polymorphism in barley. Theor. Appl. Genet. 98: 265–273.

    Article  CAS  Google Scholar 

  • Demeke, T., R.P. Adams & R.N. Chibbar, 1992. Potential use of random amplified polymorphic DNA (RAPD): A case study with Brassica. Theor. Appl. Genet. 84: 990–994.

    Article  CAS  Google Scholar 

  • Fahima, T., G.L Sun, A. Behrav, T. Kuigma, A. Beiles & E. Nevo, 1999. RAPD polymorphism of wild emmer wheat populations. Triticum dicoccoides in Israel. Theor. Appl. Genet. 98: 434–447.

    Article  CAS  Google Scholar 

  • Horejsi, T. & J.E. Staub, 1999. Genetic variation in cucumber (Cucumis sativus L.) as assessed by RAPD. Genet. Res. Crop Evol. 46: 337–350.

    Article  Google Scholar 

  • Hu, J. & C.F. Quiros, 1991. Identification of broccoli and cauliflower cultivars with RAPD markers. Plant cell Rep. 10: 505–511.

    Article  Google Scholar 

  • Isabel, N., J. Beaulieu & J. Bousquet, 1995. Complete congruence between gene diversity estimates derived from genotypic data at enzyme and random amplified polymorphic DNA loci in black spruce. Proc. Natl. Acad. Sci. USA 92: 6369–6373.

    Article  PubMed  CAS  Google Scholar 

  • Jaccard, P., 1908. Nouvelles recherché sur la distribution florale. Bull. Soc. Vaud. Sci. Natl. 44: 223–270.

    Google Scholar 

  • Jain, H.K., 1994. Pulses–The wonder plants of world agriculture, pp. 1–4 in Twenty Five Years of Pulses Research in India, edited by M. Ali, A.N. Asthana and S.L. Mehta. Indian Institute of Pulses Research, Kanpur, India.

    Google Scholar 

  • Jain, H.K. & K.L. Mehra, 1978. Evolution, adaptation, relationships and uses of the species Vigna cultivated in India, pp. 459–468 in Advances in Legume Sciences, edited by R.J. Summerfield and A.H. Bunting. Proceedings of International Legume Conference, Royal Botanic Garden, Kew.

  • Joshi, C.P. & H.T. Nguyen, 1993. Random Amplified Polymorphic DNA (RAPD) markers analyses based on intervarietal genetic relationships among hexaploid wheats. Plant Sci. 93: 95–103.

    Article  CAS  Google Scholar 

  • Koller, B., B. Lehman, J.M. McDermott & C. Cessler, 1993. Identification of apple cultivars using RAPD markers. Theor. Appl. Genet. 85: 901–904.

    Article  CAS  Google Scholar 

  • Manivannan, N., E. Murugan, P.L. Viswanathan & C.V Dhankodi, 1998. Genetic divergence in greengram. Legume Res. 21: 131–133.

    Google Scholar 

  • Saghai-Maroof, M.A., K.M. Soliman, R.A. Jorgenson & A.W. Allard, 1984. Ribosomal spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc. Natl. Acad. Sci. USA 81: 8014–8018.

    Article  PubMed  CAS  Google Scholar 

  • Sambrook, J., E.F. Fritsch & T. Maniatis, 1989. Molecular Cloning–A Laboratory Manual. 2nd edn., Cold Spring Harbor, NY, USA.

    Google Scholar 

  • Skrotch, P. & J. Nienhuis, 1995. Qualitative and quantitative characterization of RAPD variation among snap bean (Phaseolus lanatus L.) genotypes. Theor. Appl. Genet. 91: 1086–1091.

    Google Scholar 

  • Steiner, J.J., E. Piccioni, M. Falcinelli & A. Liston, 1998. Germplasm diversity among cultivars and the NPGS Crimson Clover collection. Crop Sci. 38: 263–271.

    Article  Google Scholar 

  • Tickoo, J.L. & H.K. Jain, 1988. Mung bean, pp. 161–188 in Pulse Crops, edited by B. Baldev, S. Ramanujan and H.K. Jain. Oxford & IBH Publishing, New Delhi.

    Google Scholar 

  • Tivang, J., P.W. Skrotch, J. Nienhuis & N. De Vos, 1996. RAPD variation among and within Artichoke (Cynara scolymos L.) cultivars and breeding populations. J. Amer. Soc. Hort. Sci. 121:783–788.

    CAS  Google Scholar 

  • Vavilov, N.I., 1926. The Origin, Variation, Immunity and Breeding of Cultivated Plants (Translated by K. Starr, Chester), Chronica Botanica 13. Waltham, Mass, USA.

    Google Scholar 

  • Vierling, R.A. & H.T. Nguyen, 1992. Use of RAPD markers to determine the genetic diversity of diploid wheat genotype. Theor. Appl. Genet. 84: 835–838.

    Article  CAS  Google Scholar 

  • Yang, W., A.C. Oliviera, I. Godwin, K. Schertz & J.L. Bennetzen, 1996. Comparison of DNA marker technologies in characterizing plant genome diversity: Variability in Chinese sorghums. Crop Sci. 36: 1669–1676.

    Article  Google Scholar 

  • Zhang, L.H., P. Ozias-Akins, G. Kochert, S. Kresovich, R. Dean & W. Hanna, 1999. Differentiation of bermudagrass (Cyanodon spp.) genotypes by AFLP analyses. Theor. Appl. Genet. 98: 895–902.

    Article  CAS  Google Scholar 

  • Zukovskij, P.M., 1962. Cultivated Plants and their Wild Relatives. Commonwealth Agriculture Bureau, London.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. National Research Centre on DNA Fingerprinting, National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi‐, 110 012, India

    S. Lakhanpaul, Sonia Chadha & K.V. Bhat

Authors
  1. S. Lakhanpaul
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sonia Chadha
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K.V. Bhat
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lakhanpaul, S., Chadha, S. & Bhat, K. Random amplified polymorphic DNA (RAPD) analysis in Indian mung bean (Vigna radiata (L.) Wilczek) cultivars. Genetica 109, 227–234 (2000). https://doi.org/10.1023/A:1017511918528

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1017511918528

Search

Navigation