Abstract
Several races of Fusarium oxysporum Schlechtend.:Fr f. sp. ciceris (Padwick) Matuo and K. Sato cause economic losses from wilting disease of chickpea ( Cicer arietinum L.). While the genetics of resistance to race 1 have been reported, little is known of the genetics of resistance to race 4. We undertook a study to determine the inheritance of resistance and identified random amplified polymorphic DNA markers (RAPDs) linked to the gene for resistance. For the investigation, we used 100 F5 derived F7 recombinant inbred lines (RILs) that had been developed from the cross of breeding lines C-104 x WR-315. Results indicated that resistance is controlled by a single recessive gene. The RAPD markers previously shown to amplify fragments linked to race 1 resistance also amplified fragments associated with race 4 resistance. The RAPD loci, CS-27700, UBC-170550 and the gene for resistance to race 4 segregated in 1:1 ratios expected for single genes. Both RAPD markers were located 9 map units from the race 4 resistance locus and were on the same side of the resistance gene. Our results indicated that the genes for resistance to race 1 and 4 are 5 map units apart. The need to determine the genomic locations of race specific resistance genes and the possibility that these genes are clustered to the same genomic region should be investigated.
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References
Bhatti, M.A., 1990. The effects of inoculum density and environmental factors on wilt and root rot of chickpea (Cicer arietinum L.). Ph.D. Dissertation, Department of Plant Pathology, Washington State University, Pullman, Washington. 132 p.
Buddenhagen, I.W. & F. Workneh, 1988. Fusarium wilt of chickpea in California. Phytopathol 78: 1563. (Abstract).
Burr, B., A.F. Burr, K.H. Thompson, M.C. Albertson & C.W. Stuber, 1988. Gene mapping with recombinant inbred lines in maize. Genetics 118: 519–526.
El-Hadi, M., 1993. Studies on variability in morphology, pathogenicity and vegetative compatibility of Fusarium oxysporum f. sp. ciceris, and effects of inoculum density on chickpea wilt severity. M.Sc. Thesis. Department of Plant Pathology. Washington State University, Pullman, Washington. 118 p.
Gepts, P., 1991. Biotechnology sheds light on bean domestication in Latin America. Diversity 7: 49–50.
Haglund, W.A., & J.M. Kraft, 1979. Fusarium oxysporum f. sp. pisi, race 6: Occurrence and distribution. Phytopathol 69: 818–820.
Haley, S.D., P.N. Miklas, J.R. Stavely, J. Byrum & J.D. Kelly, 1993. Identification of RAPD markers linked to a major rust resistance gene block in common bean. Theor and Appl Genet 86: 505–512.
Haware, M.P. & Y.L. Nene, 1982. Races of Fusarium oxysporum f. sp. ciceris. Plant Dis 66: 809–810.
Infantino, A., A. Porta-Pugalia & K.B. Singh, 1996. Screening wild Cicer species for resistance to fusarium wilt. Plant Dis 80: 42–44.
Jiménez-Diaz R.M., A. Trapero-Casas A & J. Cabrera de la Colina, 1989. Races of Fusarium oxysporum f. sp. ciceris infecting chickpea in southern Spain. In: Tjamos E.C. & C.H. Beckman, (eds). Vascular Wilt Diseases of Plants, pp. 515–520. NATO ASI SER. Vol. H28. Springer-Verlag, Berlin, Germany.
Kaiser W.J., A.R. Alcalá-Jiménez, A. Hervas-Vargas, J.L. Trapero-Casas & R.M. Jiménez-Diaz, 1994. Screening wild Cicer species for resistance to races 0 and 5 of Fusarium oxysporum f. sp. ciceris. Plant Dis 78: 962–967.
Kazan, K. & F.J. Muehlbauer, 1991. Allozyme variation in phylogeny in annual species of Cicer (Leguminosae). Pl Syst Evol 175: 11–21.
Keim, P., B.W. Dier, T.C. OLson & R.C. Shoemaker, 1990. RFLP mapping in soybean: association between marker loci and variation in quantitative traits. Genetics 126: 735–742.
Mayer, M.S., A. Tullu, C.J. Simon, J. Kumar, W.J. Kaiser, J.M. Kraft & F.J. Muehlbauer, 1997. Development of a DNA marker for fusarium wilt in chickpea. Crop Sci. 37: 1625–1629.
Oram, R.N., M.A.Q. Shaikh, K.M.S. Zaman, A.H.D. Brown, 1987. Isozyme similarity and genetic difference in morphology between Hypersola, a high yielding, high protein mutant of chickpea (Cicer arietinum L.) and its parental cultivar. Env Exp Bot. 27: 455–462.
Singh, H., J. Kumar, M.P. Haware & J.B. Smithson, 1987a. Genetics of resistance to fusarium wilt in chickpeas. In: Day P.R. & G.J. Jellis (eds). Genetics and Plant Pathogenesis, (pp. 339–341) Blackwell Scientific Publications, Oxford. (Poster Abstracts).
Suiter, K., J. Wendel & J. Case, 1983. Linkage-1: A pascal computer program for the detection and analysis of genetic linkage. J Hered 74: 203–204.
Trapero-Casas, A. & R.M. Jiménez-Diaz, R.M., 1985. Fungal wilt and root rot diseases of chickpea in southern Spain. Phytopathol 75: 1146–1151.
Upadhyaya, H.D., J.B. Smithson, M.P. Haware & J. Kumar, 1983a. Resistance to wilt in chickpea. I. Inheritance of late wilting in response to race 1. Euphytica 32: 447–452.
Upadhyaya, H.D., J.B. Smithson, J. Kumar & M.P. Haware, 1983b. Resistance to wilt in chickpea. II. Further evidence for two genes for resistance to race 1. Euphytica 32: 749–755.
Wechter, W.P., M.P. Whitehead, C.E. Thomas & R.A. Dean, 1995. Identification of a randomly amplified polymorphic DNA marker linked to the Fom 2 fusarium wilt resistance gene in muskmelon MR-1. Mol Plant Pathol 85: 1245–1249.
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Tullu, A., Muehlbauer, F., Simon, C. et al. Inheritance and linkage of a gene for resistance to race 4 of fusarium wilt and RAPD markers in chickpea. Euphytica 102, 227–232 (1998). https://doi.org/10.1023/A:1018373412281
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DOI: https://doi.org/10.1023/A:1018373412281