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Pathogenicity and Lipid Composition of Mycelium of the Fungus Stagonospora cirsii VIZR 1.41 during Submerged Cultivation

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Abstract

In order to identify qualitative markers of a potential mycoherbicide against Canada thistle based on the mycelium of the fungus Stagonospora cirsii VIZR 1.41, changes in its pathogenicity and lipid profiles during submerged cultivation on liquid sucrose-soybean medium were analyzed. The fatty-acid composition of major lipids and the identification of molecular species of structural lipids were determined. The lipid composition during fungal growth changed together with its pathogenicity. The highly pathogenic mycelium of the fungus (at the log phase of growth) was characterized with the relatively highest content of structural lipids: phosphatidylcholine (PC), phosphatidylethanolamine (PE), and ergosterol (ES). Their relative concentration decreased with culture age and a decrease in pathogenicity. The highest lipid yield was found at the beginning of stationary growth phase (at the maximal biomass yield) of S. cirsii; triacylglycerides (TAGs) were the most abundant. The double-bond index of fatty acids in glycerolipids decreased with culture age, and the substitution of molecular PC species (34:2 versus 36:4) and PE (36:4 versus 34:2) was observed as well. High levels of ES, TAG, PC (in particular, 34:2 forms) and PE (36:4 forms) were presumed to be qualitative markers of S. cirsii mycelium.

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ACKNOWLEDGMENTS

The authors are grateful to M. Z. Muradymov (Botanical Institute), M. A. Vinogradskaya (Botanical Institute), and A. V. Rubizhan (VIZR) for their substantial research assistance.

Funding

This work was carried out with the financial support of the Russian Science Foundation (project No. 16-16-00085) on the equipment of the Innovative Plant Protection Technologies Center for Collective Use (VIZR), as well as the research centers Development of Molecular and Cellular Technologies and Chemical Analysis and Materials of St. Petersburg State University.

The methodological basis of the protocol for the analysis of molecular species of glycoceramides and sterols was developed during the implementation of the topic “Assessment of changes in the correlation structure of metabolic networks during the growth and development of fungi and plants from the standpoint of system biology” of the Botanical Institute of the Russian Academy of Sciences (AAAA-A18-118032390136-5).

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Authors and Affiliations

  1. All-Russian Institute of Plant Protection, 196608, Pushkin, St. Petersburg, Russia

    G. M. Frolova, S. V. Sokornova & A. O. Berestetskiy

  2. Botanical Institute, 197376, St. Petersburg, Russia

    E. R. Kotlova, S. V. Senik & A. L. Shavarda

  3. Chemical Analysis and Materials Research Center, St. Petersburg State University, 198504, St. Petersburg, Russia

    A. D. Misharev

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  1. G. M. Frolova
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  2. E. R. Kotlova
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  3. S. V. Sokornova
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  4. S. V. Senik
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  5. A. L. Shavarda
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  6. A. D. Misharev
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  7. A. O. Berestetskiy
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Correspondence to A. O. Berestetskiy.

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The authors declare that they have no conflict of interest. The study was performed without the use of animals or people as subjects.

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Translated by P. Kuchina

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Frolova, G.M., Kotlova, E.R., Sokornova, S.V. et al. Pathogenicity and Lipid Composition of Mycelium of the Fungus Stagonospora cirsii VIZR 1.41 during Submerged Cultivation. Appl Biochem Microbiol 57, 226–235 (2021). https://doi.org/10.1134/S0003683821020034

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