Skip to main content
SpringerLink
Log in

Bacterial Diversity and Functional Activity of Microbial Communities in Hot Springs of the Baikal Rift Zone

  • Experimental Articles
  • Published:
Microbiology Aims and scope Submit manuscript

Abstract

In this study the bacterial diversity of thermophilic microbial mats (40 to 65°C) in three alkaline hot springs of the Baikal Rift Zone (BRZ) was determined through pyrosequencing of 16S rRNA gene libraries. Significant diversity of bacterial species was found in the biomats of the hot springs with total number of detected phylotypes of 607. The highest share of the microbial community was represented by the phyla Chloroflexi (Seya Spring, 76.4%), Deinococcus-Thermus (Alla Spring, 45.1%), Nitrospira (Alla Spring, 36.1%), Cyanobacteria (Tsenkher Spring, 33.1%), and Proteobacteria (Tsenkher Spring, 22.6%), but their ratio varied significantly in different springs. A comparison of the biodiversity and composition of microbial communities between hot springs showed a decrease in biodiversity with increasing temperature. A large number of sequences showed a low degree of similarity with cultivated representatives in public databases. Microbial communities showed intensive rates of production and destruction of organic compounds, as revealed by the quantitative assessment of their functional activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Badhai, J., Ghosh, T.S., and Das, S.K., Taxonomic and functional characteristics of microbial communities and their correlation with physicochemical properties of four geothermal springs in Odisha, India, Front. Microbiol., 2015, vol. 6, p. 1166. doi 10.3389/fmicb.2015.01166

    Google Scholar 

  • Bolhuis, H., Cretoiu, M.S., and Stal, L.J., Molecular ecology of microbial mats, FEMS Microbiol. Ecol., 2014, vol. 90, pp. 335–350.

    CAS  PubMed  Google Scholar 

  • Bonch-Osmolovskaya, E.A., Thermophilic microorganisms: a general overview, Proc. Winogradsky Inst. Microbiol., Thermophilic microorganisms, Galchenko, V.F., Ed., Moscow: MAKS, 2011, pp. 5–14.

    Google Scholar 

  • Bonch-Osmolovskaya, E.A., Miroshnichenko, M.L., Slobodkin, A.I., Sokolova, T.G., Karpov, G.A., Kostrikina, N.A., Zavarzina, D.G., Prokofeva, M.I., Rusanov, I.I., and Pimenov, N.V., Biodiversity of anaerobic lithotrophic prokaryotes in terrestrial hot springs of Kamchatka, Microbiology (Moscow), 1999, vol. 68, pp. 343–351.

    CAS  Google Scholar 

  • Boomer, S.M., Noll, K.L., Geesey, G.G., and Dutton, B.E., Formation of multilayered photosynthetic biofilms in an alkaline thermal spring in Yellowstone National Park, Wyoming, Appl. Environ. Microbiol., 2009, vol. 75, pp. 2464–2475.

    Article  CAS  PubMed  Google Scholar 

  • Chun, J., Kim, K.Y., Lee, J.-H., and Choi, Y., The analysis of oral microbial communities of wild-type and toll-like receptor 2-deficient mice using a 454 GS FLX Titanium pyrosequencer, BMC Microbiol., 2010, vol. 10, article 101.

  • Cole, J.K., Peacock, J.P., Dodsworth, J.A., Williams, A.J., Thompson, D.B., Dong, H., Wu, G., and Hedlund, B.P., Sediment microbial communities in Great Boiling Spring are controlled by temperature and distinct from water communities, ISME J., 2013, vol. 7, pp. 718–729.

    Article  CAS  PubMed  Google Scholar 

  • Coman, C., Drugă, B., Hegedus, A., Sicora, C., and Dragoş, N., Archaeal and bacterial diversity in two hot spring microbial mats from a geothermal region in Romania, Extremophiles, 2013, vol. 17, pp. 523–534.

    Article  PubMed  Google Scholar 

  • Costa, K.C., Navarro, J.B., Shock, E.L., Zhang, C.L., Soukup, D., and Hedlund, B.P., Microbiology and geochemistry of great boiling and mud hot springs in the United States Great Basin, Extremophiles, 2009, vol. 13, pp. 447–459.

    Article  CAS  PubMed  Google Scholar 

  • De Leon, K.B., Gerlach, R., Peyton, B.M., and Fields, M.W., Archael and bacterial communities in three alkaline hot springs in heart lake Geyser Basin, Yellowstone National Park, Front. Microbiol., 2013, vol. 4, pp. 1–10.

    CAS  Google Scholar 

  • Everroad, R.C., Otaki, H., Matsuura, K., and Haruta S., Diversification of bacterial community composition along a temperature gradient at a thermal spring, Microbes Environ., 2012, vol. 27, pp. 374–381.

    Article  PubMed  PubMed Central  Google Scholar 

  • Gorlenko, V.M., Bonch-Osmolovskaya, E.A., Kompantseva, E.I., and Starynin, D.A., Production of organic matter in microbial mats of the Thermophilny Spring in the Uzon Caldera in Kamchatka, Microbiology (Moscow), 1987, vol. 56, pp. 692–697.

    Google Scholar 

  • Gumerov, V.M., Mardanov, A.V., Beletsky, A.V., Bonch-Osmolovskaya, E.A., and Ravin, N.V., Molecular analysis of microbial diversity in the Zavarzin Spring, Uzon Caldera, Kamchatka, Microbiology (Moscow), 2011, vol. 80, pp. 244–251.

    Article  CAS  Google Scholar 

  • Hedlund, B.P., Cole, J.K., Williams, A.J., Hou, W., Zhou, E., Li, W., and Dong, H., A review of the microbiology of the Rehai geothermal field in Tengchong, Yunnan Province, China, Geosci. Frontiers, 2012, vol. 3, no. 3, pp. 273–288.

    Article  Google Scholar 

  • Hou, W., Wang, S., Dong, H., Jiang, H., Briggs, B.R., Peacock, J.P., Huang, Q., Huang, L., Wu, G., Zhi, X., Li, W., Dodsworth, J.A., Hedlund, B.P., Zhang, C., Hartnett, H.E., Dijkstra, P., and Hungate, B.A., A comprehensive census of microbial diversity in hot springs of Tengchong, Yunnan Province China using 16S rRNA gene pyrosequencing, PLoS One, 2013, vol. 8, no. 1. p. e53350.

    Google Scholar 

  • Huang, Q., Dong, C.Z., Dong, R.M., Jiang, H., Wang, S., Wang, G., Fang, B., Ding, X., Niu, L., Li, X., Zhang, C., and Dong, H., Archaeal and bacterial diversity in hot springs on the Tibetan Plateau, China, Extremophiles, 2011, vol. 15, pp. 549–563.

    Article  Google Scholar 

  • Ionescu, D., Hindiyeh, M., Malkawi, H., and Oren, A., Biogeography of thermophilic cyanobacteria: insights from the Zerka Ma’in hot springs (Jordan), FEMS Microbiol. Ecol., 2010, vol. 72, pp. 103–113.

    Article  CAS  PubMed  Google Scholar 

  • Kanokratana, P., Chanapan, S., Pootanakit, K., and Eurwilaichitr, L., Diversity and abundance of Bacteria and Archaea in the Bor Khlueng hot spring in Thailand, J. Bas. Microbiol., 2004, vol. 44, pp. 430–444.

    Article  Google Scholar 

  • Klatt, C.G., Inskeep, W.P., Herrgard, M.J., Jay, Z.J., Rusch, D.B., Tringe, S.G., Parenteau, M.N., Ward, D.M., Boomer, S.M., Bryant, D.A., and Miller, S.R., Community structure and function of high-temperature chlorophototrophic microbial mats inhabiting diverse geothermal environments, Front. Microbiol., 2013, vol. 4, p. 106.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Klatt, C.G., Wood, J.M., Rusch, D.B., Bateson, M.M., Hamamura, N., Heidelberg, J.F., Grossman, A.R., Bhaya, D., Cohan, F.M., Kühl, M., Bryant, D.A., and Ward, D.M., Community ecology of hot spring cyanobacterial mats: predominant populations and their functional potential, ISME J., 2011, vol. 5, pp. 1262–1278.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kublanov, I.V., Perevalova, A.A., Slobodkina, G.B., Lebedinsky, A.V., Bidzhieva, S.K., Kolganova, T.V., Kaliberda, E.N., Rumsh, L.D., Haertlé, T., and Bonch-Osmolovskaya, E.A., Biodiversity of thermophilic prokaryotes with hydrolytic activities in hot springs of Uzon Caldera, Kamchatka (Russia), Appl. Environ. Microbiol., 2009, vol. 75, pp. 286–291.

    Article  CAS  PubMed  Google Scholar 

  • Lau, C.Y., Aitchison, J.C., and Pointing, S.B., Bacterial community composition in thermophilic microbial mats from five hot springs in central Tibet, Extremophiles, 2009, vol. 13, pp. 139–149.

    Article  PubMed  Google Scholar 

  • Lin, W., Christian, J., Schüler, D., and Pan, Y., Metagenomic analysis reveals unexpected subgenomic diversity of magnetotactic bacteria within the phylum Nitrospirae, Appl. Environ. Microbiol., 2011, vol. 77, no. 1, pp. 323–326.

    Article  CAS  PubMed  Google Scholar 

  • Loginova, L.G., Egorova, L.A., Golovacheva, R.S., and Seregina L.M., Thermus ruber sp. nov., nom. rev., Int. J. Sys. Bacteriol., 1984, vol. 344, pp. 498–499.

    Article  Google Scholar 

  • Mackenzie, R., Pedrós-Alió, C., and Díez, B., Bacterial composition of microbial mats in hot springs in Northern Patagonia: variations with seasons and temperature, Extremophiles, 2013, vol. 17, pp. 123–136.

    Article  PubMed  Google Scholar 

  • Madigan, M.T., Bacterial habitats of extremophiles, in Journey to Diverse Microbial Worlds, vol. 2, Seckbach, J., Ed., Boston: Kluwer Academic, 2000, pp. 61–72.

    Chapter  Google Scholar 

  • Meyer-Dombard, D.R. and Amend, J.P., Geochemistry and microbial ecology in alkaline hot springs of Ambitle Island, Papua New Guinea, Extremophiles, 2014, vol. 18, no. 4, pp. 763–778.

    CAS  PubMed  Google Scholar 

  • Meyer-Dombard, D.R., Shock, E.L., and Amend, J.P., Archaeal and bacterial communities in geochemically diverse hot springs of Yellowstone National Park, USA, Geobiology, 2005, vol. 3, pp. 211–227.

    Article  Google Scholar 

  • Miller, S.R., Strong, A.L., Jones, K.L., and Ungerer, M.C., Bar-coded pyrosequencing reveals shared bacterial community properties along the temperature gradients of two alkaline hot springs in Yellowstone National Park, Appl. Environ. Microbiol., 2009, vol. 75, pp. 4565–4572.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Nakagawa, T. and Fukui, M., Phylogenetic characterization of microbial mats and streamers from a Japanese alkaline hot spring with a thermal gradient, J. Gen. Appl. Microbiol., 2002, vol. 48, pp. 211–222.

    Article  CAS  PubMed  Google Scholar 

  • Namsaraev, B.B., Gorlenko, V.M., Namsaraev, Z.B., Barkhutova, D.D., Kozyreva, L.P., Dagurova, O.P., and Tatarinov, A.V., Evolutionary aspects of geochemical activity of microbial mats in lakes and hydrotherms of Baikal Rift Zone, in Biosphere Origin and Evolution, Dobretsov, N., Kolchanov, N., Rozanov, A., and Zavarzin, G., Eds., New York: 2008, pp. 189–202.

    Chapter  Google Scholar 

  • Pimenov, N.V. and Bonch-Osmolovskaya, E.A., In situ activity studies in thermal environments, Methods Microbiol., 2006, vol. 35, pp. 29–54.

    Article  CAS  Google Scholar 

  • Purcell, D., Sompong, U., Lau, C.Y., Barraclough, T.G., Peerapornpisal, Y., and Pointing, S.B., The effects of temperature, pH and sulphide on the community structure of hyperthermophilic streamers in hot springs of northern Thailand, FEMS Microbiol. Ecol., 2007, vol. 60, no. 3, pp. 456–466.

    Article  CAS  PubMed  Google Scholar 

  • Revsbech, N.P. and Ward, D.M., Microelectrode studies of interstitial water chemistry and photosynthetic activity in a hot spring microbial mat, Appl. Environ. Microbiol., 1984, vol. 48, pp. 270–275.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Sharp, C.E., Brady, A.L., Sharp, G.H., Grasby, S.E., Stott, M.B., and Dunfield, P.F., Humboldt’s spa: microbial diversity is controlled by temperature in geothermal environments, ISME J., 2014, vol. 8, pp. 1166–1174.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Song, Z.Q., Wang, F.P., Zhi, X.Y., Chen, J.Q., Zhou, E.M., Liang, F., Tang, S.K., Jiang, H.C., Zhang, C.L., Dong, H., and Li, W.J., Bacterial and archaeal diversities in Yunnan and Tibetan hot springs, China, Environ. Microbiol., 2013, vol. 15, no. 4, pp. 1160–1175.

    Article  CAS  Google Scholar 

  • Tobler, D.J. and Benning, L.G., Bacterial diversity in five Icelandic geothermal waters: temperature and sinter growth rate effects, Extremophiles, 2011, vol. 15, pp. 473–485.

    Article  PubMed  Google Scholar 

  • Tomova, I., Stoilova-Disheva, M., Lyutskanova, D., Pascual, J., Petrov, P., and Kambourova, M., Phylogenetic analysis of the bacterial community in a geothermal spring, Rupi Basin, Bulgaria, World J. Microbiol. Biotechnol., 2010, vol. 26, pp. 2019–2028.

    Article  Google Scholar 

  • Vick, T.J., Dodsworth, J.A., Costa, K.C., Shock, E.L., and Hedlund, B.P., Microbiology and geochemistry of Little Hot Creek, a hot spring environment in the Long Valley Caldera, Geobiology, 2010, vol. 8, pp. 140–154.

    Article  CAS  PubMed  Google Scholar 

  • Wang, S., Hou, W., Dong, H., Jiang, H., Huang, L., Wu, G., Zhang, C., Song, Z., Zhang, Y., Ren, H., Zhang, J., and Zhang, L., Control of temperature on microbial community structure in hot springs of the Tibetan Plateau, PLoS One, 2013, vol. 8, no. 5, p. e62901.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ward, D.M., Ferris, M.J., Nold, S.C., and Bateson, M.M., A natural view of microbial biodiversity within hot spring cyanobacterial mat communities, Microbiol. Mol. Biol. Rev., 1998, vol. 62, pp. 1353–1370.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Yamada, T., Imachi, H., Ohashi, A., Harada, H., Hanada, S., Kamagata, Y., and Sekiguchi, Y., Bellilinea caldifistulae gen. nov., sp. nov. and Longilinea arvoryzae gen. nov., sp. nov., strictly anaerobic, filamentous bacteria of the phylum Chloroflexi isolated from methanogenic propionate-degrading consortia, Int. J. Syst. Evol. Microbiol., 2007, vol. 57, no. 10, pp. 2299–2306.

    Article  CAS  PubMed  Google Scholar 

  • Zavarzin, G.A., Lectures on Natural History Microbiology, Moscow: Nauka, 2003.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of General and Experimental Biology, Siberian Branch, Russian Academy of Sciences, Ulan-Ude, Russia

    E. V. Lavrentyeva, A. A. Radnagurueva, D. D. Barkhutova, S. V. Zaitseva & B. B. Namsaraev

  2. Buryat State University, Ulan-Ude, Russia

    E. V. Lavrentyeva

  3. Limnological Institute, Siberian Branch, Russian Academy of Sciences, Irkutsk, Russia

    N. L. Belkova

  4. National Research Centre “Kurchatov Institute”, Moscow, Russia

    Z. B. Namsaraev

  5. The Federal Research Centre “Fundamentals of Biotechnology”, Russian Academy of Sciences, Moscow, Russia

    V. M. Gorlenko

Authors
  1. E. V. Lavrentyeva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. A. Radnagurueva
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. D. Barkhutova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. N. L. Belkova
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. S. V. Zaitseva
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Z. B. Namsaraev
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. V. M. Gorlenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. B. B. Namsaraev
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to E. V. Lavrentyeva.

Additional information

The article is published in the original.

† Deceased.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Lavrentyeva, E.V., Radnagurueva, A.A., Barkhutova, D.D. et al. Bacterial Diversity and Functional Activity of Microbial Communities in Hot Springs of the Baikal Rift Zone. Microbiology 87, 272–281 (2018). https://doi.org/10.1134/S0026261718020078

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0026261718020078

Keywords

Search

Navigation