Abstract—
The type strain Rhodotorula pinalis VKM Y-2963 has been characterized genetically and phylogenetically. The rDNA sequences obtained for the ITS region and D1/D2 domains of LSU have been deposited to NCBI GenBank (OM666053). Strains from the Moscow region, Fellozyma sp. KBP 3851 from Sphagnum mosses and Rh. pinalis VKM Y-2963 from dead conifer needles, were found to be conspecific. The results of phylogenetic analysis suggested that the species Rh. pinalis should be reassigned to the genus Fellozyma. The new combination, Fellozyma pinalis comb. nov., is proposed, MycoBank MB842988.
Similar content being viewed by others
REFERENCES
Boekhout, T., Aime, M.C., Begerow, D., Gabaldón, T., Heitman, J., Kemler, M., Khayhan, K., Lachance, M.-A., Louis, E.J., Sun, S., Vu, D., Yurkov, A., The evolving species concepts used for yeasts: from phenotypes and genomes to speciation networks, Fungal Diversity, 2021b, vol. 109, pp. 27–55.
Boekhout, T., Amend, A.S., El Baidouri, F., Gabaldón, T., Geml, J., Mittelbach, M., Robert, V., Tan, C.S., Turchetti, B., Vu, D., Wang, Q.-M., and Yurkov, A., Trends in yeast diversity discovery, Fungal Diversity, 2021a. https://doi.org/10.1007/s13225-021-00494-6
Fell, J.W., Boekhout, T., Fonseca, A., Scorzetti, G., and Statzell-Tallman, A., Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis, Int. J. Syst. Evol. Microbiol., 2000, vol. 50, pp. 1351–1371.
Golubev, W.I., A new myo-inositol-positive species of the genus Rhodotorula, Mikol. Fitopatol., 2010, vol. 44, pp. 310–314.
Kachalkin, A.V., Glushakova, A.M., Yurkov, A.M., and Chernov, I.Yu., Characterization of yeast groupings in the phyllosphere of sphagnum mosses, Microbiology (Moscow), 2008, vol. 77, pp. 474–481.
Katoh, K., Rozewicki, J., and Yamada, K.D., MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization, Brief. Bioinform., 2019, vol. 20, pp. 1160–1166.
Kurtzman, C.P. and Robnett, C.J., Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences, Antonie van Leeuwenhoek, 1998, vol. 73, pp. 331–371.
Kuznetsova, T.A., Vecherskii, M.V., Khayrullin, D.R., Stepankov, A.A., Maximova, I.A., Kachalkin, A.V., and Ushakova, N.A., Dramatic effect of black soldier fly larvae on fungal community in a compost, J. Sci. Food Agric., 2022, vol. 102, pp. 2598–2603. https://doi.org/10.1002/jsfa.11601
Li, A.H., Yuan, F.X., Groenewald, M., Bensch, K., Yurkov, A.M., Li, K., Han, P.J., Guo, L.D., Aime, M.C., Sampaio, J.P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q.-M., and Bai, F.-Y., Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Stud. Mycol., 2020, vol. 96, pp. 17–140.
Liu, X.Z., Wang, Q.-M., Göker, M., Groenewald, M., Kachalkin, A.V., Lumbsch, H.T., Millanes, A.M., Wedin, M., Yurkov, A.M., Boekhout, T., and Bai, F.-Y., Towards an integrated phylogenetic classification of the Tremellomycetes, Stud. Mycol., 2015a, vol. 81, pp. 85–147.
Liu, X.Z., Wang, Q.-M., Theelen, B., Groenewald, M., Bai, F.-Y., and Boekhout, T., Phylogeny of tremellomycetous yeasts and related dimorphic and filamentous basidiomycetes reconstructed from multiple gene sequence analyses, Stud. Mycol., 2015b, vol. 81, pp. 1‒26.
Lücking, R., Aime, M.C., Robbertse, B., Miller, A.N., A-oki, T., Ariyawansa, H.A., Cardinali, G., Crous, P.W., Druzhinina, I.S., Geiser, D.M., Hawksworth, D.L., Hyde, K.D., Irinyi, L., Jeewon, R., Johnston, P.R., et al., Fungal taxonomy and sequence-based nomenclature, Nat. Microbiol., 2021, vol. 6, pp. 540–548.
McNeill, J., Barrie, F., Buck, W., Demoulin, V., Greuter, W., Hawksworth, D., et al., International Code of Nomenclature for Algae, Fungi, and Plants (Melbourne Code), Königstein: Koeltz Scientific Books, 2012.
Schoch, C.L., Seifert, K.A, Huhndorf, S., Robert, V., Spouge, J.L., Levesque, C.A., Chen, W., and Fungal Barcoding Consortium, Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi, Proc. Natl. Acad. Sci. U. S. A., 2012, vol. 109, pp. 6241–6246.
Scorzetti, G., Fell, J.W., Fonseca, A., and Statzell-Tallman, A., Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions, FEMS Yeast Res., 2002, vol. 2, pp. 495–517.
Tamura, K., Stecher, G., Peterson, D., Filipski, A., and Kumar, S., MEGA6: Molecular Evolutionary Genetics Analysis version 6.0, Mol. Biol. Evol., 2013, vol. 30, pp. 2725–2729.
Tedersoo, L., Bahram, M., Põlme, S., Kõljalg, U., Yorou, N.S., Wijesundera, R., Villarreal Ruiz, L., Vasco-Palacios, A.M., Thu, P.Q., Suija, A., Smith, M.E., Sharp, C., Saluveer, E., Saitta, A., Rosas, M., et al., Fungal biogeography. Global diversity and geography of soil fungi, Science, 2014, vol. 346, art. 1256688.
Vu, D., Groenewald, M., Szöke, S., Cardinali, G., Eberhardt, U., Stielow, B., de Vries, M., Verkleij, G.J., Crous, P.W., Boekhout, T., and Robert, V., DNA barcoding analysis of more than 9000 yeast isolates contributes to quantitative thresholds for yeast species and genera delimitation, Stud. Mycol., 2016, vol. 85, pp. 91–105.
Wang, Q.-M., Groenewald, M., Takashima, M., Theelen, B., Han, P.J., Liu, X.Z., Boekhout, T., and Bai, F.-Y., Phylogeny of yeasts and related filamentous fungi within Pucciniomycotina determined from multigene sequence analyses, Stud. Mycol., 2015b, vol. 81, pp. 27–53.
Wang, Q.-M., Yurkov, A.M., Göker, M., Lumbsch, H.T., Leavitt, S.D., Groenewald, M., Theelen, B., Liu, X.Z., Boekhout, T., and Bai, F.-Y., Phylogenetic classification of yeasts and related taxa within Pucciniomycotina, Stud. Mycol., 2015a, vol. 81, pp. 149–189.
Funding
The study was supported by the Ministry of Science and Higher Education of the Russian Federation, agreement no. 075-15-2021-1051.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.
Additional information
Translated by E. Makeeva
Rights and permissions
About this article
Cite this article
Kachalkin, A.V. Phylogeny of Rhodotorula pinalis and Its Reclassification as Fellozyma pinalis comb. nov.. Microbiology 91, 417–420 (2022). https://doi.org/10.1134/S0026261722100915
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0026261722100915