Abstract
Changes in total phenolics content (TPC) and total antioxidant activity (TAA) in different morphogenic stages of Tacitus bellus (L.) Moran and J. Meyrán (Crassulaceae) direct shoot organogenesis in vitro were examined. A HPLC method was used for the assessment of changes in phenolic profile. Significant decrease in TPC and TAA, as well as the decline of the amount of specific phenolic compounds coincided with the initiation of shoot organogenesis. Additional decrease in TPC and TAA, and the disappearance of procyanidin glycoside, occurred associated with shoot development. Performed correlation analysis suggests phenolics as the main component of nonenzymatic antioxidant system involved in T. bellus direct shoot organogenesis, it argues in favor of involvement of specific phenolics in the regulation of early stages of T. bellus direct shoot organogenesis and indicates different fine regulatory mechanisms of early and late stages of shoot organogenesis.
Abbreviations
- BAP:
-
benzylaminopurine
- CAT:
-
catalase
- SOD:
-
superoxide dismutase
- POD:
-
peroxidase
- PPO:
-
polyphenol oxidase
- NAA:
-
naphtaleneacetic acid
- TAA:
-
total antioxidant activity
- TPC:
-
total phenolics content
References
Sugimoto, K., Gordon, S.P., and Meyerowitz, E.M., Regeneration in plants and animals: dedifferentiation, transdifferentiation, or just differentiation? Trends Cell Biol., 2011, vol. 21, pp. 212–218.
Reinert, J. and Bajaj, Y.P.S., Applied and Fundamental Aspects of Plant Cell, Tissue and Organ Culture, New York: Springer-Verlag, 1977.
Gordon, S.P., Chickarmane, V.S., Ohno, C., and Meyerowitz, E.M., Multiple feedback loops through cytokinin signaling control stem cell number within the Arabidopsis shoot meristem, Proc. Natl. Acad. Sci. U.S.A., 2009, vol. 106, pp. 16529–16534.
Su, Y.H., Liu, Y.B., and Zhang, X.S., Auxin–cytokinin interaction regulates meristem development, Mol. Plant, 2011, vol. 4, pp. 616–625.
Koes, R., Verweij, W., and Quattrocchio, F., Flavonoids: a colourful model for the regulation and evolution of biochemical pathways, Trends Plant Sci., 2005, vol. 10, pp. 236–242.
Franklin, G. and Dias, A.C.P., Chlorogenic acid participates in the regulation of shoot, root and root hair development in Hypericum perforatum, Plant Physiol. Biochem., 2011, vol. 49, pp. 835–842.
Arnaldos, T.L., Munoz, R., Ferrer, M.A., and Calderon, A.A., Changes in phenol content during strawberry (Fragaria × ananassa, cv. Chandler) callus culture, Physiol. Plant., 2001, vol. 113, pp. 315–322.
Jacobs, M. and Rubery, P.H., Naturally occuring auxin transport regulators, Science, 1988, vol. 241, pp. 346–349.
Peer, W.A. and Murphy, A.S., Flavonoids and auxin transport: modulators or regulators? Trends Plant Sci., 2007, vol. 12, pp. 556–563.
Peer, W.A., Blakeslee, J.J., Yang, H., and Murphy, A.S., Seven things we think we know about auxin transport, Mol. Plant, 2011, vol. 4, pp. 487–504.
Agarwal, M. and Kamal, R., Studies on flavonoid production using in vitro cultures of Momordica charantia L., Ind. J. Biotechnol., 2007, vol. 6, pp. 277–279.
Shilpashree, H.P. and Raim, R., In vitro plant regeneration and accumulation of flavonoids in Hypericum mysorense, Int. J. Integr. Biol., 2009, vol. 8, pp. 43–49.
Subhashini, Devi, P.S., Satyanarayanam, B., Arundhati, A., and Raghava, Rao, T., Activity of antioxidant enzymes and secondary metabolites during in vitro regeneration of Sterculia urens, Biol. Plant., 2013, vol. 57, pp. 778–782.
Mitrović, A., Janošević, D., Budimir, S., and Bogdanović, Pristov, J., Changes in antioxidative enzymes activities during Tacitus bellus direct shoot organogenesis, Biol. Plant., 2012, vol. 56, pp. 357–361.
Murashige, T. and Skoog, F., A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plant., 1962, vol. 15, pp. 473–497.
Arnao, M.B., Cano, A., and Acosta, M., Methods to measure the antioxidant activity in plant material. A comparative discussion, Free Radic. Res., 1999, vol. 32, pp. 89–96.
Singleton, V.L. and Rossi, J.A., Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents, Am. J. Enol. Vitic., 1965, vol. 16, pp. 144–158.
Kanmegene, G. and Omokolo, D., Changes in phenol content and peroxidase activity during in vitro organogenesis in Xanthosoma sagittifolium L., Plant Growth Regul., 2003, vol. 40, pp. 53–57.
Cheng, Z.H., Wang, L., Sun, W., Zhang, Y., Zhou, C., Sum, Y.H., Li, W., Sun, T.T., Zhao, X.Y., Li, X.G., Cheng, Y., Zhao, Y., Xie, Q., and Zhang, X.S., Pattern of auxin and cytokinin responses for shoot meristem induction results from the regulation of cytokinin biosynthesis by AUXIN RESPONSE FACTOR3, Plant Physiol., 2013, vol. 161, pp. 240–251.
Hiraga, S., Sasaki, K., Ito, H., Ohashi, Y., and Matsui, H., A large family of class III plant peroxidases, Plant Cell Physiol., 2001, vol. 42, pp. 462–468.
Cervilla, L.M., Rosales, M.A., Rubio-Wilhelmi, M.M., Sanchez-Rodriguez, E., Blasco, B., Rios, J.J., Romero, L., and Ruiz, J.M., Involvement of lignification and membrane permeability in the tomato root response to boron toxicity, Plant Sci., 2009, vol. 176, pp. 545–552.
Author information
Authors and Affiliations
Corresponding author
Additional information
The article is published in the original.
Rights and permissions
About this article
Cite this article
Mitrović, A., Maksimović, V., Mutavdžić, D. et al. Total phenol content and total antioxidant activity drop during Tacitus bellus direct shoot organogenesis. Russ J Plant Physiol 62, 700–705 (2015). https://doi.org/10.1134/S102144371505012X
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S102144371505012X