Abstract
Stationary phases based on titania are obtained by the adsorptive attachment of polyethyleneimine followed by crosslinking with 1,4-butanediol diglycidyl ether. The chromatographic properties of the adsorbents are studied using the Tanaka test for hydrophilic stationary phases and on an example of a separation of a model mixture of water-soluble B vitamins. It is shown that varying the amount of reagents at the stage of synthesis of the stationary phase affects the structure of the functional layer and thereby makes it possible to obtain a adsorbent with improved chromatographic characteristics.
Similar content being viewed by others
REFERENCES
Alpert, A.J., J. Chromatogr. A, 1990, vol. 499, p. 177.
Huber, J.F.K., Pawlowska, M., and Markl, P., J. Chromatogr. A, 1990, vol. 500, p. 257.
Chernobrovkina, A.V., Smolenkov, A.D., and Shpigun, O.A., Lab. Proizvod., 2018, no. 4, p. 76.
Bather, J.M. and Gray, R.A., J. Chromatogr. A, 1978, vol. 156, p. 21.
Jaffrezic-Renault, N., Poirier-Andrade, H., and Trang, D.H., J. Chromatogr. A, 1980, vol. 201, p. 187.
Kawahara, C., Kozbur, X., Gould, S., and Parker, L., Anal. Biochem., 1980, vol. 102, p. 310.
Kawahara, M., Nakamura, H., and Nakajima, T., J. Chromatogr. A, 1990, vol. 515, p. 149.
Grun, M., Kurganov, A.A., Schacht, S., Schuth, F., and Unger, K.K., J. Chromatogr. A, 1996, vol. 740, p. 1.
Tani, K. and Suzuki, Y., Chromatographia, 1997, vol. 46, p. 623.
Tani, K. and Kubojima, H., Chromatographia, 1998, vol. 47, p. 655.
Tani, K. and Ozawa, M., J. Liq. Chromatogr. Relat. Technol., 1999, vol. 22, p. 843.
Tani, K., Sumizawa, T., Watanabe, M., Tachibana, M., Koizumi, H., and Kiba, T., Chromatographia, 2002, vol. 55, p. 33.
Pinkse, M.W., Uitto, P.M., Hilhorst, M.J., Ooms, B., and Heck, A.J., Anal. Chem., 2004, vol. 76, p. 3935.
Ting, Z. and Lucy, C.A., J. Chromatogr. A, 2008, vol. 1187, p. 87.
Ting, Z. and Lucy, C.A., J. Chromatogr. A, 2010, vol. 1217, p. 82.
Ozawa, M., Tani, K., Nomura, A., Tachibana, M., Koizumi, H., and Kiba, N., Chromatographia, 2009, vol. 70, p. 533.
Ozawa, M., Tani, K., Tachibana, M., Koizumi, H., and Kiba, N., Chromatographia, 2010, vol. 72, p. 313.
Moliner-Martinez, Y., Herráez-Hernández, R., Molins-Legua, C., and Campins-Falco, P., J. Chromatogr. A, 2010, vol. 1217, p. 4682.
Tan, J., Li, R., and Jiang, Z.T., Anal. Methods, 2011, vol. 3, p. 1568.
Abi, J.M. and Randon, J., J. Chromatogr. A, 2011, vol. 1218, p. 721.
Chicz, R.M., Shi, Z., and Regnier, F.E., J. Chromatogr. A, 1986, vol. 359, p. 121.
Alpert, A.J. and Regnier, F.E., J. Chromatogr. A, 1979, vol. 185, p. 375.
Segro, S.S., Cabezas, Y., and Malik, A., J. Chromatogr. A, 2009, vol. 1216, p. 4329.
Kawachi, Y., Ikegami, T., Takubo, H., Ikegami, Y., Miyamoto, M., and Tanaka, N., J. Chromatogr. A, 2011, vol. 1218, p. 5903.
Dolci, M., Chromatographic Characterization of Stationary Phases for Hydrophilic Interaction Liquid Chromatography, Cheshire, UK: Thermo Fisher Scientific, 2013.
Buszewski, B. and Noga, S., Anal. Bioanal. Chem., 2012, vol. 402, p. 231.
Kip, C., Tosun, R.B., Alpaslan, S., Koçer, I., Çelik, E., and Tuncel, A., Talanta, 2019, vol. 200, p. 100.
Kavathekar, R.S. English, N.J., and Mac-Elroy, J.M.D., Chem. Phys. Lett., 2012, vol. 554, p. 102.
Funding
This work was supported by M.V.Lomonosov Moscow State University Program of Development and by the Russian Foundation for Basic Research, project no. 20-03-00909.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated by V. Kudrinskaya
Rights and permissions
About this article
Cite this article
Popov, A.S., Tsarenko, E.A. & Chernobrovkina, A.V. Titania-Based Adsorbents for Hydrophilic Interaction Liquid Chromatography. J Anal Chem 76, 1153–1162 (2021). https://doi.org/10.1134/S1061934821100087
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1061934821100087