Abstract
Photosystem II (PSII) is a multicomponent enzyme complex that catalyzes the light-induced water splitting to molecular oxygen, protons, and electrons. Photosystem II is located in the membranes of cyanobacteria, green algae, and plants. The crystallization of this complex from the thylakoid membranes poses great difficulties. The high sensitivity of photosystem II to light and radiation has an adverse effect on the crystal quality, as well as on the quality of X-ray diffraction data. This is the reason why the crystal structure of PSII from Thermosynechococcus elongates has as yet not been determined at high resolution. The optimization of the strategy for collecting X-ray diffraction data from PSII crystals has resulted in an increase in the resolution to 2.75 Å, which made it possible to determine the positions of ions and some water molecules playing an important role in the functioning of PSII.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
J. Kern, B. Loll, C. Lüneberg, et al., Biochim. Biophys. Acta 1706, 147 (2005).
A. G. W. Leslie and H. R. Powell, Evolving Methods Macromol. Crystallogr. 245, 41 (2007).
W. Kabsch, J. Appl. Crystallogr. 26, 795 (1993).
A. J. McCoy, R. W. Grosse-Kunstleve, P. D. Adams, et al., J. Appl. Crystallogr. 40, 658 (2007).
E. Potterton, P. Briggs, M. Turkenburg, et al., Acta Crystallogr. D 59, 1131 (2003).
A. Guskov, J. Kern, A. Gabdulkhakov, et al., Nat. Struct. Mol. Biol. 16, 334 (2009).
P. V. Afonine, R. W. Grosse-Kunstleve, N. Echols, et al., Acta Crystallogr. D 68, 352 (2012).
P. Emsley, B. Lohkamp, W. G. Scott, et al., Acta Crystallogr. D 66, 486 (2010).
R. A. Laskowski, M. W. MacArthur, D. S. Moss, et al., J. Appl. Crystallogr. 26, 283 (1993).
R. W. Hooft, G. Vriend, C. Sander, et al., Nature 381, 272 (1996).
A. G. Gabdulkhakov, M. V. Dontsova, and W. Saenger, Crystallogr. Rep. 56, 1054 (2011).
K. Kawakami, Y. Umena, N. Kamiya, et al., Proc. Natl. Acad. Sci. USA 106, 8567 (2009).
J. W. Murray, Energy Environ. Sci. 1, 161 (2008).
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © A.G. Gabdulkhakov, M.V. Dontsova, 2014, published in Kristallografiya, 2014, Vol. 59, No. 1, pp. 80–82.
Rights and permissions
About this article
Cite this article
Gabdulkhakov, A.G., Dontsova, M.V. Preliminary X-ray diffraction study of crystals of photosystem II from Thermosynechococcus elongates . Crystallogr. Rep. 59, 75–77 (2014). https://doi.org/10.1134/S1063774514010040
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1063774514010040