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Expression of human serum albumin in methylotrophic yeast Pichia pastoris and its structural and functional analysis

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Abstract

The stable strain of methylotrophic yeast Pichia pastoris secreting human serum albumin into cultural medium was obtained. Optimal conditions for expression of the protein were determined. We characterized the recombinant protein by mass spectrometry and circular dichroism and analyzed its catalytic activity.

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Abbreviations

HSA:

human serum albumin

AOX:

alcohol oxidase

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Authors and Affiliations

  1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia

    T. V. Bobik, I. I. Vorob’ev, N. A. Ponomarenko, A. G. Gabibov & A. I. Miroshnikov

Authors
  1. T. V. Bobik
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  2. I. I. Vorob’ev
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  3. N. A. Ponomarenko
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  4. A. G. Gabibov
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  5. A. I. Miroshnikov
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Correspondence to T. V. Bobik.

Additional information

Original Russian Text © T.V. Bobik, I.I. Vorob’ev, N.A. Ponomarenko, A.G. Gabibov, A.I. Miroshnikov, 2008, published in Bioorganicheskaya Khimiya, 2008, Vol. 34, No. 1, pp. 56–62.

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Bobik, T.V., Vorob’ev, I.I., Ponomarenko, N.A. et al. Expression of human serum albumin in methylotrophic yeast Pichia pastoris and its structural and functional analysis. Russ J Bioorg Chem 34, 49–55 (2008). https://doi.org/10.1134/S1068162008010068

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  • DOI: https://doi.org/10.1134/S1068162008010068

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