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The structure of the O-specific polysaccharide from a mutant of nitrogen-fixing rhizobacterium Azospirillum brasilense Sp245 with an altered plasmid content

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Abstract

The rhizobacteria Azospirillum brasilense Sp245 produce immunochemically different lipopolysaccharides LPSI and LPSII, both containing identical pentasaccharides built from D-rhamnose residues as the repeating units of O-specific polysaccharides (OPS). In this study, we report the structure of the OPS from A. brasilense LPSI−LPSII− mutant Sp245.5, which spontaneously lost the p85 and p120 plasmids upon the formation of a new 300-MDa megaplasmid after the long-term storage of the bacteria in a rich medium. The repeating unit of the OPS of A. brasilense Sp245.5 appeared to be a disaccharide consisting of residues of N-acetyl-D-galactosamine and N-acetyl-D-mannosaminuronic acid:

$$ \to 6) - \alpha - D - GalpNAc - (1 \to 4) - \beta - D - ManpNAcA - (1 \to $$

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Abbreviations

CBPS:

Calcofluor-binding polysaccharides

CC:

coupling constant

COSY:

correlation spectroscopy

CPS:

capsular polysaccharides

EPS:

exopolysaccharides

HMBS:

heteronuclear multiple-bond correlation spectroscopy

HSQC:

heteronuclear single-quantum coherence spectroscopy

LPS:

lipopolysaccharide

OPS:

O-specific polysaccharide

ROESY:

rotating-frame nuclear Overhauser enhancement spectroscopy

TOCSY:

total correlation spectroscopy

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Authors and Affiliations

  1. Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, pr. Entuziastov 13, Saratov, 410049, Russia

    Yu. P. Fedonenko, E. I. Katsy, L. P. Petrova & A. S. Boyko

  2. N.D. Zelinskii Institute of Organic Chemistry, Russian Academy of Sciences, Leninskii pr. 47, Moscow, 119991, Russia

    E. L. Zdorovenko, V. V. Kachala, A. S. Shashkov & Yu. A. Knirel

Authors
  1. Yu. P. Fedonenko
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  2. E. I. Katsy
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  3. L. P. Petrova
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  4. A. S. Boyko
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  5. E. L. Zdorovenko
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  6. V. V. Kachala
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  7. A. S. Shashkov
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  8. Yu. A. Knirel
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Corresponding author

Correspondence to Yu. P. Fedonenko.

Additional information

Original Russian Text © Yu.P. Fedonenko, E.I. Katsy, L.P. Petrova, A.S. Boyko, E.L. Zdorovenko, V.V. Kachala, A.S. Shashkov, Yu.A. Knirel, 2010, published in Bioorganicheskaya Khimiya, 2010, Vol. 36, No. 2, pp. 236–240.

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Fedonenko, Y.P., Katsy, E.I., Petrova, L.P. et al. The structure of the O-specific polysaccharide from a mutant of nitrogen-fixing rhizobacterium Azospirillum brasilense Sp245 with an altered plasmid content. Russ J Bioorg Chem 36, 219–223 (2010). https://doi.org/10.1134/S1068162010020111

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  • DOI: https://doi.org/10.1134/S1068162010020111

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