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Proteolysis of simple glyprolines by leucine aminopeptidase and enzymes from nasal mucus, brain membranes, and blood of rats

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Abstract

Proteolysis of Pro-Gly-Pro-Leu, Pro-Gly-Pro-Gly and Pro-Gly-Pro compared to Met-Glu-His-Phe-Pro-Gly-Pro (Semax) has been studied. It is shown that all three peptides are significantly more resistant to N-leucine aminopeptidase (EC 3.4.11.1, Sigma, type VI, 9.2 units/mg), as well as to enzymes of the nasal mucus, the microsomal fraction of brain, and blood of rats. Metabolites of the proteolysis showed that semax derives is His-Phe-Pro-Gly-Pro only; Pro-Gly-Pro-Leu forms Gly-Pro-Leu, Pro-Gly-Pro, and Gly-Pro; Pro-Gly-Pro-Gly gives Pro-Gly-Pro and Gly-Pro; and Pro-Gly-Pro forms Gly-Pro.

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Abbreviations

MERB:

membrane enzymes of rat brain

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Authors and Affiliations

  1. Institute of Molecular Genetics of the Russian Academy of Sciences, 123182, Moscow, Kurchatov pl. 2, Russia

    K. V. Shevchenko, T. V. V’yunova, I. Yu. Nagaev, L. A. Andreeva & N. F. Myasoedov

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  1. K. V. Shevchenko
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  2. T. V. V’yunova
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  3. I. Yu. Nagaev
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  4. L. A. Andreeva
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  5. N. F. Myasoedov
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Correspondence to K. V. Shevchenko.

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Original Russian Text © K.V. Shevchenko, T.V. V’yunova, I.Yu. Nagaev, L.A. Andreeva, N.F. Myasoedov, 2013, published in Bioorganicheskaya Khimiya, 2013, Vol. 39, No. 3, pp. 320–325.

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Shevchenko, K.V., V’yunova, T.V., Nagaev, I.Y. et al. Proteolysis of simple glyprolines by leucine aminopeptidase and enzymes from nasal mucus, brain membranes, and blood of rats. Russ J Bioorg Chem 39, 283–287 (2013). https://doi.org/10.1134/S1068162013030151

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  • DOI: https://doi.org/10.1134/S1068162013030151

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