Adapted from a report presented at the VI Russian Symposium “Proteins and Peptides” (June 11–15, 2013)
Abstract
Microcapsules composed of synthetic (sodium polystyrene sulfonate and polyallylamine hydrochloride) and biodegradable polyelectrolytes (dextran sulfate and polyarginine hydrochloride) deposited on carbonate microparticles have been obtained. The ultrastructural organization of biodegradable microcapsules has been studied by transmission electron microscopy. The shell of biodegradable microcapsules is well formed even after the deposition of six polyelectrolyte layers and has an average thickness of 44 ± 3.0 nm; their inner polyelectrolyte matrix is less branched than that of synthetic microcapsules. By using spectroscopy, the efficiency of the encapsulation of FITC-labeled BSA by adsorption depending on the number of PE layers in the capsule has been estimated. It has been shown that the maximum amount of the protein is incorporated into capsules comprising six and seven polyelectrolyte layers (4 and 2 pg/capsule, respectively). It has been concluded that the adsorption of proteins into preformed polyelectrolyte capsules enables one to avoid protein losses that occur with the method in which biomineral cores obtained by coprecipitation are used for encapsulation.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- BSA:
-
bovine serum albumin
- DS:
-
dextran sulfate
- EDTA:
-
ethylenediaminetetraacetic acid
- FITC:
-
fluorescein isothiocyanate
- IPEC:
-
interpolyelectrolyte complex
- PAH:
-
polyallylamine hydrochloride
- PAr:
-
polyarginine hydrochloride
- PE:
-
polyelectrolyte
- PEMC:
-
polyelectrolyte microcapsule
References
Sukhorukov, G.B., Donath, E., Davis, S., Lichtenfeld, H., Caruso, F., Popov, V.I., and Mohwald, H., Polym. Adv. Technol., 1998, vol. 9, pp. 759–767.
Inozemtseva, O.A., Portnov, S.A., Kolesnikova, T.A., and Gorin, D.A., Ross. Nanotekhnol., 2007, vol. 2, pp. 68–80.
Dubrovskii, A.V., Shabarchina, L.I., Kim, Yu.A., and Sukhorukov, B.I., Zh. Fiz. Khim., 2006, vol. 80, pp. 1914–1919.
Voigt, A., Lichtenfeld, G.B., Sukhorukov, G.B., Zastrov, H., Donath, E., Baumler, H., and Mohwald, H., Ind. Eng. Chem. Res., 1999, vol. 38, pp. 4037–4043.
Donath, E., Sukhorukov, G.B., Caruso, F., Davis, S.A., and Mohwald, H., Angew. Chem., Int. Ed. Engl., 1998, vol. 37, pp. 2202–2205.
Volodkin, D.V., Petrov, A.I., Petrov, M., and Sukhorukov, G.B., Langmuir, 2004, vol. 20, pp. 3398–3406.
Kazakova, L.I., Dubrovskii, A.V., Moshkov, D.A., Shabarchina, L.I., and Sukhorukov, B.I., Biofizika, 2007, vol. 52, pp. 850–854.
Studer, D., Palankar, R., Bédard, M., Winterhalter, M., and Springer, S., Small, 2010, vol. 6, pp. 2412–2419.
Kazakova, L.I., Shabarchina, L.I., and Sukhorukov, G.B., Phys. Chem. Chem. Phys., 2011, vol. 13, pp. 11110–11117.
Del Mercato, L.L., Abbasi, A.Z., Ochs, M., and Parak, W.J., ACS Nano, 2011, vol. 5, pp. 9668–9674.
De Koker, S., De Cock, L.J., Rivera-Gil, P., Parak, W.J., Velty, R.A., Vervaet, C., Remon, J.P., and De Geest, B.J., Adv. Drug Deliv. Rev., 2011, vol. 63, pp. 748–761.
De Geest, B.J., Dejugnat, C., Verhoeven, E., Sukhorukov, G.B., Jonas, A.V., Plain, J., Demeester, J., and De Smedt, S.C., J. Controlled Rerlease, 2006, vol. 116, pp. 159–169.
De Koker, S., De Geest, B.J., Singh, S.K., De Rycke, R., Naessens, T., Van Kooyk, Y., Demeester, J., and De Smedt, S.C., Angewandte Chemie (Int. Ed.), 2009, vol. 48, pp. 8485–8489.
Borodina, T.N., Rumsh, L.D., Kunizhev, S.M., Sukhorukov, G.B., Vorozhtsov, G.N., Fel’dman, B.M., Rusanova, A.V., Vasil’eva, T.V., Strukova, S.M., and Markvicheva, E.A., Biomed. Khim., 2007, vol. 53, pp. 662–671.
Borodina, T.N., Rumsh, L.D., Kunizhev, S.M., Sukhorukov, G.B., Vorozhtsov, G.N., Fel’dman, B.M., and Markvicheva, E.A., Biomed. Khim., 2007, vol. 53, pp. 557–565.
She, Zhen, Antipina, M.N., Li, Jun, and Sukhorukov, G.B., Biomacromolecules, 2010, vol. 11, pp. 1241–1247.
Volodkin, D.V., Larionova, N.I., and Sukhorukov, G.B., Biomacromolecules, 2004, vol. 5, pp. 1962–1972.
Qi, W., Yan, X.H., Juan, L., Cui, Y., and Li, J.B., Biomacromolecules, 2009, vol. 10, pp. 1212–1216.
Antipov, A.A., Sukhorukov, G.B., Donat, E., and Möhwald, H., J. Phys. Chem. B, 2001, vol. 105, pp. 2281–2284.
Balabushevich, N.G., Tiourina, O.P., Volodkin, D.V., Larionova, N.I., and Sukhorukov, G.B., Biomacromolecules, 2003, vol. 4, pp. 1191–1197.
Tiourina, O.P., Antipov, A.A., Sukhorukov, G.B., Larionova, N.I., Lvov, Y., and Möhwald, H., Macromol. Biosci., 2001, vol. 1, pp. 209–214.
Antipov, A.A., Shchukin, D., Fedutik, Y., Petrov, A.I., Sukhorukov, G.B., and Mohwald, H., Colloid. Surf.: Physicochem. Eng. Aspect, 2003, vol. 224, pp. 175–184.
Sukhorukov, G.B., Volodkin, D.V., Gunther, A.M., Petrov, A.I., Shenoy, D.V., and Möhwald, H., J. Mater. Chem., 2004, vol. 14, pp. 2073–2081.
Petrov, A.I., Volodkin, D.V., and Sukhorukov, G.B., Biotechnol. Prog., 2005, vol. 21, pp. 918–925.
Kazakova, L.I., Dubrovskii, A.V., Santalova, I.M., Moshkov, D.A., Apolonnik, N.V., and Shabarchina, L.I., Russ. J. Bioorg. Chem., 2012, vol. 38, pp. 51–55.
Balabushevich, N.G., Sukhorukov, G.B., and Larionova, N.I., Vestn. Mosk. Univ. Ser. 2 Khim., 2002, vol. 43, pp. 374–377.
Moshkov, D.A., in Adaptatsiya i ul’trastruktura neirona (Adaptation and Neuron Ultrastructure), Moscow: Nauka, 1985.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © O.Yu. Kochetkova, L.I. Kazakova, D.A. Moshkov, M.G. Vinokurov, L.I. Shabarchina, 2013, published in Bioorganicheskaya Khimiya, 2013, Vol. 39, No. 5, pp. 565–571.
Rights and permissions
About this article
Cite this article
Kochetkova, O.Y., Kazakova, L.I., Moshkov, D.A. et al. Incorporation of proteins into polyelectrolyte microcapsules by coprecipitation and adsorption. Russ J Bioorg Chem 39, 504–509 (2013). https://doi.org/10.1134/S1068162013050087
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1068162013050087