Skip to main content
SpringerLink
Log in

The use of H/D exchange for secondary structure characterization of supermetallized complexes of ubiquitin with cerium(III)

  • Published:
Russian Journal of Bioorganic Chemistry Aims and scope Submit manuscript

Abstract

The approach of hydrogen/deuterium exchange combined with ultrahigh resolution mass spectrometry was applied for investigation of conformational changes of supermetallized ubiquitin ions with cerium(III) atoms. The dependencies of the hydrogen/deuterium exchange efficiency on the charge state of ubiquitin ion, the number of associated cerium atoms, as well as on the temperature were obtained. The reaction of hydrogen/deuterium exchange was performed directly in the ionization source according to previously described method. It was found that the number of exchanges is hardly altered under the addition of cerium atoms. This result indirectly suggests that the conformation of small protein supermetallized ions does not significantly change during electrospray ionization.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

ICR:

ion cyclotron resonance

References

  1. Zawisza, I., Rozga, M., and Bal, W., Coordination Chem. Rev., 2012, vol. 256, nos. 19–20, pp. 2297–2307.

    Article  CAS  Google Scholar 

  2. Taraszka, J.A., Li, J.W., and Clemmer, D.E., J. Phys. Chem. B, 2000, vol. 104, no. 18, pp. 4545–4551.

    Article  CAS  Google Scholar 

  3. Viles, J.H., Coordination Chem. Rev., 2012, vol. 256, nos. 19–20, pp. 2271–2284.

    Article  CAS  Google Scholar 

  4. Kozlowski, H., Luczkowski, M., Remelli, M., and Valensin, D., Coordination Chem. Rev., 2012, vol. 256, nos. 19–20, pp. 2129–2141.

    Article  CAS  Google Scholar 

  5. Miller, Y., Ma, B.Y., and Nussinov, R., Coordination Chem. Rev., 2012, vol. 256, nos. 19–20, pp. 2245–2252.

    Article  CAS  Google Scholar 

  6. Carlton, D.D. and Schug, K.A., Anal. Chim. Acta, 2011, vol. 686, nos. 1–2, pp. 19–39.

    Article  CAS  PubMed  Google Scholar 

  7. Farkas, E. and Sovago, I., Metal Complexes of Amino Acids and Peptides, in Amino Acid., Peptides and Proteins, Cambridge: RSC Publishing, 2012, vol. 37, pp. 66–118.

    CAS  Google Scholar 

  8. Faller, P., Hureau, C., Dorlet, P., Hellwig, P., Coppel, Y., Collin, F., and Alies, B., Coordination Chem. Rev., 2012, vol. 256, nos. 19–20, pp. 2381–2396.

    Article  CAS  Google Scholar 

  9. Kostyukevich, Y., Kononikhin, A., Popov, I., Indeykina, M., Kozin, S.A., Makarov, A.A., and Nikolaev, E., J. Mass Spectrometry, 2015, vol. 50, no. 9, pp. 1079–1087.

    Article  CAS  Google Scholar 

  10. Katta, V. and Chait, B.T., J. Am. Chem. Soc., 1993, vol. 115, no. 14, pp. 6317–6321.

    Article  CAS  Google Scholar 

  11. Wales, T.E. and Engen, J.R., Mass Spectrometry Rev., 2006, vol. 25, no. 1, pp. 158–170.

    Article  CAS  Google Scholar 

  12. Kostyukevich, Y., Kononikhin, A., Popov, I., and Nikolaev, E., Anal. Chem., 2013, vol. 85, no. 11, pp. 5330–5334.

    Article  CAS  PubMed  Google Scholar 

  13. Kostyukevich, Y., Kononikhin, A., Popov, I., and Nikolaev, E., J. Mass Spectrometry, 2014, vol. 49, no. 10, pp. 989–994.

    Article  CAS  Google Scholar 

  14. Kostyukevich, Y., Kononikhin, A., Popov, I., Spasskiy, A., and Nikolaev, E., J. Mass Spectrometry, 2015, vol. 50, no. 1, pp. 49–55.

    Article  CAS  Google Scholar 

  15. Kostyukevich, Y., Kononikhin, A., Popov, I., and Nikolaev, E., J. Mass Spectrometry, 2015, vol. 50, no. 10, pp. 1150–1156.

    Article  CAS  Google Scholar 

  16. Kostyukevich, Y., Kononikhin, A., Popov, I., and Nikolaev, E., Anal. Chem., 2014, vol. 86, no. 5, pp. 2595–2600.

    Article  CAS  PubMed  Google Scholar 

  17. Kostyukevich, Y., Kononikhin, A., Popov, I., Kharybin, O., Perminova, I., Konstantinov, A., and Nikolaev, E., Anal. Chem., 2013, vol. 85, no. 22, pp. 11007–11013.

    Article  CAS  PubMed  Google Scholar 

  18. Kostyukevich, Y., Kononikhin, A., Zherebker, A., Popov, I., Perminova, I., and Nikolaev, E., Anal. Bioanal. Chem., 2014, vol. 406, no. 26, pp. 6655–6664.

    Article  CAS  PubMed  Google Scholar 

  19. Zherebker, A.Y., Airapetyan, D., Konstantinov, A.I., Kostyukevich, Y.I., Kononikhin, A.S., Popov, I.A., Zaitsev, K.V., Nikolaev, E.N., and Perminova, I.V., Analyst, 2015, vol. 140, no. 13, pp. 4708–4719.

    Article  CAS  PubMed  Google Scholar 

  20. Kostyukevich, Y., Kononikhin, A., Popov, I., and Nikolaev, E., Eur. J. Mass Spectrometry, 2015, vol. 21, no. 2, pp. 109–113.

    Article  CAS  Google Scholar 

  21. Kostyukevich, Y., Kononikhin, A., Popov, I., Starodubtseva, N., Kukaev, E., and Nikolaev, E., Eur. J. Mass Spectrometry, 2014, vol. 20, no. 4, pp. 345–349.

    Article  CAS  Google Scholar 

  22. Kostyukevich, Y., Kononikhin, A., Popov, I., Starodubtzeva, N., Pekov, S., Kukaev, E., Indeykina, M., and Nikolaev, E., Eur J. Mass Spectrometry, 2015, vol. 21, no. 1, pp. 59–63.

    Article  CAS  Google Scholar 

  23. Koeniger, S.L., Merenbloom, S.I., and Clemmer, D.E., J. Phys. Chem. B, 2006, vol. 110, no. 13, pp. 7017–7021.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Skolkovo Institute of Science and Technology, ul. Novaya 100, Skolkovo, Moscow oblast, 143025, Russia

    Yu. Kostyukevich & E. Nikolaev

  2. Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Leninskii pr. 38, k. 2, Moscow, 119334, Russia

    Yu. Kostyukevich, A. Kononikhin & E. Nikolaev

  3. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 119334, Russia

    Yu. Kostyukevich, I. Popov & A. Bugrova

  4. Moscow Institute of Physics and Technology, State University, Institutskii per. 9, Dolgoprudny, Moscow oblast, 141700, Russia

    P. Yacovlev, A. Kononikhin, I. Popov, N. Starodubtzeva & E. Nikolaev

  5. Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, ul. Oparina 4, Moscow, 117997, Russia

    A. Kononikhin, A. Bugrova & N. Starodubtzeva

Authors
  1. Yu. Kostyukevich
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. Yacovlev
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Kononikhin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. I. Popov
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. Bugrova
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. N. Starodubtzeva
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. E. Nikolaev
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to E. Nikolaev.

Additional information

Original Russian Text © Yu. Kostyukevich, P. Yacovlev, A. Kononikhin, I. Popov, A. Bugrova, N. Starodubtzeva, E. Nikolaev, 2016, published in Bioorganicheskaya Khimiya, 2016, Vol. 42, No. 5, pp. 539–545.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kostyukevich, Y., Yacovlev, P., Kononikhin, A. et al. The use of H/D exchange for secondary structure characterization of supermetallized complexes of ubiquitin with cerium(III). Russ J Bioorg Chem 42, 484–490 (2016). https://doi.org/10.1134/S1068162016040117

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1068162016040117

Keywords

Search

Navigation