Abstract
We have proposed previously a template-assisted method for 3D assembly of nucleic acid nanostructures. The method involved a branched oligonucleotide as a template and a building block, which incorporates non-nucleotidic linkers, in particular, branching foci connecting two or three oligonucleotide chains. In this work, we have attempted the synthesis of branched oligonucleotide templates for the assembly of a DNA tetrahedron and a DNA cube, a branched oligonucleotide building block (“starlet”), and studied the assembly of a DNA tetrahedron.
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The work was supported by the Russian Foundation for Basic Research (grant nos. 16-03-01055 and 18-29-08062), and the Ministry of Science and Higher Education of the Russian Federation (Novosibirsk State University project FSUS-2020-0035).
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Abbreviations: BCN, bicyclо[6.1.0]non-4-yne; ВР, Bromophenol Blue; DMTr, 4,4'-dimethoxytrityl; EtBr, ethidium bromide; MMTr, 4-methoxytrityl; NPOM, 1-(2-nitropiperonyl)ethoxymethyl; SPAAC, strain-promoted azide-alkyne [3+2]cycloaddition; TEAA, triethylammonium acetate; THPTA, tris-hydroxypropyltriazolylmethylamine; ОN, oligonucleotide; BО, branched oligonucleotide; BОT, branched oligonucleotide template; BОB, branched oligonucleotide building block; DE, disconnecting element; prefixes d and r in deoxy- and 2'-O-methylribonucleotides are omitted.
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Fokina, A.A., Poletaeva, Y.E., Burakova, E.A. et al. Template-Assisted Assembly of DNA Nanostructures from Branched Oligonucleotides. Russ J Bioorg Chem 47, 700–712 (2021). https://doi.org/10.1134/S1068162021030067
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DOI: https://doi.org/10.1134/S1068162021030067