Skip to main content
SpringerLink
Log in

The Effect of Low and Medium Doses of Proton Pencil Scanning Beam on the Blood-Forming Organs during Total Irradiation of Mice

  • BIOCHEMISTRY, BIOPHYSICS, AND MOLECULAR BIOLOGY
  • Published:
Doklady Biochemistry and Biophysics Aims and scope Submit manuscript

Abstract

The aim of this work was to study the effect of proton pencil beam scanning in the Bragg peak in the dose range of 0.1–1.5 Gy on the induction of cytogenetic damage in the bone marrow, reactive oxygen species (ROS) production in whole blood, and the state of lymphoid organs after total body irradiation of mice. Irradiation was carried out in the Prometeus proton synchrotron (Protvino) in the Bragg peak with proton energy at the output of 90–116 MeV. It was found that, under irradiation of mice in the range of low and medium doses of proton pencil beam scanning in the Bragg peak, the relative biological effectiveness (RBE) according to the criterion of cytogenetic changes was 1.15. In addition, it was found that the pathophysiological effect on the lymphoid organs and the production of ROS by blood cells were different as compared with the effect of X-rays.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.

Similar content being viewed by others

REFERENCES

  1. Paganetti, H., Blakely, E., Carabe-Fernandez, A., et al., Report of the AAPM TG-256 on the relative biological effectiveness of proton beams in radiation therapy, Med. Phys., 2019, vol. 46, no. 3, pp. 53–78.

    Google Scholar 

  2. Luhr, A., Neubeck, C., Krause, M., et al., Relative biological effectiveness in proton beam therapy—current knowledge and future challenges, Clin. Transl. Radiat. Oncol., 2018, vol. 9, pp. 35–41.

    PubMed  PubMed Central  Google Scholar 

  3. Khmelevskii, E.V., Radiation therapy for prostate cancer: photons, protons, or heavy ions?, Radiats. Onkol. Yad. Med., 2013, no. 1, pp. 28–33.

  4. Held, K.D., Kawamura, H., Kaminuma, T., et al., Effects of charged particles on human tumor cells, Front. Oncol., 2016, vol. 6, art. 23.

    PubMed  PubMed Central  Google Scholar 

  5. Ivanov, A.A., Bychkova, T.M., Nikitenko, O.V., et al., Radiobiological effects of protons, Med. Radiol. Radiats. Bezop., 2019, vol. 64, no. 3, pp. 19–31.

    Google Scholar 

  6. Direktiva 2010/63/eu Evpopeiskogo parlamenta i Soveta Evpopeiskogo soyuza ot 22 sentyabrya 2010 g. po okhrane zhivotnykh, ispol’zuemykh v nauchnykh tselyakh (Directive 2010/63/EU of the European Parliament and the Council of the European Union of September 22, 2010, on the Protection of Animals Used for Scientific Purposes), St. Petersburg: Rus-LASA “NP ob’’edinenie spetsialistov po rabote s laboratornymi zhivotnymi,” rabochaya gruppa po perevodam i izdaniyu tematicheskoi literatury, 2012.

  7. Klokov, D.Yu., Patterns of formation of radiation adaptive response in bone marrow cells of mice in vivo, Cand. Sci. (Biol.) Dissertation, 2000.

  8. Tommansino, F. and Durante, M., Proton radiobiology, Cancers, 2015, pp. 353–381.

  9. Calugaru, V., Nauraye, C., Noel, G., Giocanti, N., et al., Radiobiological characterization of two therapeutic proton beams with different initial energy spectra used at the Institut Curie Proton Therapy Center in Orsay, Inst. J. Radiat. Oncol. Biol. Phys., 2011, vol. 81, no. 4, pp. 1136–1143.

    Google Scholar 

  10. Finnberg, N., Wambi, C., Ware, J.H., et al., Gamma-radiation (GR) triggers a unique gene expression profile associated with cell death compared to proton radiation (PR) in mice in vivo, Cancer Biol. Ther., 2008, vol. 7, no. 12, pp. 2023–2033.

    CAS  PubMed  Google Scholar 

  11. Giedzinski, E., Rola, R., Fike, J.R., et al., Efficient production of reactive oxygen species in neural precursor cells after exposure to 250 MeV protons, Radiat. Res., 2005, vol. 164, no. 4, pp. 540–544.

    CAS  PubMed  Google Scholar 

  12. Naranga, H., Kumara, A., Bhat, N., et al., Effect of proton and gamma irradiation on human lung carcinoma cells: gene expression, cell cycle, cell death, epithelial-mesenchymal transition and cancer-stem cell trait as biological end points, Mutat. Res., 2015, vol. 780, pp. 35–46.

    Google Scholar 

Download references

Funding

The effect of low and medium doses of protons on the hematopoietic organs during total irradiation of mice was studied using pencil proton beam scanning technology at the Physical Technical Center of Lebedev Physical Institute and at the Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, within the framework of basic research under state assignment topics and with partial support by the R&D agreement no. 01/19 between the Physical Technical Center of Lebedev Physical Institute and the Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences.

Author information

Authors and Affiliations

  1. Physical Technical Center, Lebedev Physical Institute, Russian Academy of Sciences, Protvino, Moscow oblast, Russia

    V. E. Balakin, T. A. Belyakova & A. E. Shemyakov

  2. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, Russia

    O. M. Rozanova, E. N. Smirnova, A. E. Shemyakov, S. I. Zaichkina, S. S. Sorokina & A. R. Dyukina

Authors
  1. V. E. Balakin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. O. M. Rozanova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. N. Smirnova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T. A. Belyakova
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. E. Shemyakov
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. S. I. Zaichkina
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. S. S. Sorokina
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. A. R. Dyukina
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to O. M. Rozanova or T. A. Belyakova.

Ethics declarations

Conflict of interest. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. All manipulations with mice were performed in accordance with the regulatory legal acts on the experimental work with animals [6], and the experimental plan was approved by the Commission on Biological Safety and Bioethics of the Institute of Theoretical and Experimental Biophysics (Protocol no. 4 from April 5, 2018).

Additional information

Translated by M. Batrukova

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Balakin, V.E., Rozanova, O.M., Smirnova, E.N. et al. The Effect of Low and Medium Doses of Proton Pencil Scanning Beam on the Blood-Forming Organs during Total Irradiation of Mice. Dokl Biochem Biophys 494, 231–234 (2020). https://doi.org/10.1134/S1607672920050026

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1607672920050026

Keywords:

Search

Navigation